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Tolerance to various environmental stresses conferred by the salt-responsive rice gene ONAC063 in transgenic Arabidopsis

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Abstract

Environmental stresses limit plant growth and crop production worldwide. We attempted to isolate rice genes involved in conferring tolerance to environmental stresses by using a transgenic Arabidopsis population expressing full-length cDNAs of rice. Among these lines, a thermotolerant line, R08946, was detected. The rice cDNA inserted in R08946 encoded a NAC transcription factor, ONAC063. This protein was localized in the nucleus and showed transactivation activity at the C-terminus. ONAC063 expression was not induced by high-temperature but highly induced by high-salinity in rice roots. High-osmotic pressure and reactive oxygen species levels also induced ONAC063 expression. The seeds of ONAC063-expressing transgenic Arabidopsis showed enhanced tolerance to high-salinity and osmotic pressure. Microarray and real-time reverse transcription-polymerase chain reaction analyses showed upregulated expression of some salinity-inducible genes, including the amylase gene AMY1, in ONAC063-expressing transgenic Arabidopsis. Thus, ONAC063 may play an important role in eliciting responses to high-salinity stress.

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Acknowledgments

This work was supported in part by Special Coordination Funds for Promoting Science and Technology (Science and Technology Agency of Japan). We thank Dr. M. Mori (National Institute of Agrobiological Sciences) for providing the rice seeds, and Dr. T. Nakagawa (Shimane University, Japan) for providing the pGWB5 plasmid.

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Authors and Affiliations

  1. Research Institute for Biological Sciences (RIBS), Okayama, 7549-1 Yoshikawa, Kibichuo-cho, Okayama, 716-1241, Japan

    Naoki Yokotani, Masaki Iwabuchi & Kenji Oda

  2. Plant Functional Genomics Research Team, Plant Functional Genomics Research Group, Plant Science Center, RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan

    Takanari Ichikawa, Youichi Kondou & Minami Matsui

  3. Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

    Hirohiko Hirochika

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  1. Naoki Yokotani
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  4. Minami Matsui
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  5. Hirohiko Hirochika
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Correspondence to Kenji Oda.

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425_2009_895_MOESM1_ESM.pdf

Fig. S1 Expression of known stress-related genes in transgenic Arabidopsis expressing ONAC063. Transcription levels are expressed relative to the level of transcripts from Col-0, which is assumed to be one. Data are represented as mean ± SD of the values obtained from triplicate experiments (PDF 28 kb)

Table S1 Sequences of the primers used for real-time PCR analysis. (PDF 15 kb)

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Yokotani, N., Ichikawa, T., Kondou, Y. et al. Tolerance to various environmental stresses conferred by the salt-responsive rice gene ONAC063 in transgenic Arabidopsis. Planta 229, 1065–1075 (2009). https://doi.org/10.1007/s00425-009-0895-5

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