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Marker-aided selection and validation of various \({ Pi}\) gene combinations for rice blast resistance in elite rice variety ADT 43

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Abstract

Rice blast caused by fungal pathogen Pyricularia oryzae has a major impact on reducing yield potential of rice. In this study, homozygous plants were selected using microsatellite markers from the \(\hbox {BC}_{3}\hbox {F}_{2}\) population pyramided with four major genes in elite rice variety ADT 43. Background and selected lines with various blast resistance gene combinations were screened under natural conditions to study the effects of various gene combinations. Upon inspection of lines with different gene combinations, the three-gene pyramided line Pi54+Pi33+Pi1 was found to be highly resistant with the score of 3.3 followed by other three-gene pyramided lines Pi54+Pi2+Pi1 and Pi33+Pi2+Pi1, with the scores of 3.9 and 3.8, respectively. Two-gene pyramided lines Pi54+Pi1, Pi33+Pi1 and Pi2+Pi1 were found to be moderately resistant with a mean score of 4.0 each. In the case of monogenic lines, positive plants for Pi54 performed almost equal to three-gene pyramided lines with a mean score of 3.6. Lines with Pi2 and Pi1 were found to be moderately resistant and moderately susceptible with the mean scores of 4.1 and 4.5, respectively.

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Authors and Affiliations

  1. Department of Agronomy, Kansas State University, Throckmorton Plant Sciences Center, Manhattan, KS, 66506, USA

    Balaji Aravindhan Pandian

  2. Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, 641 003, India

    John Joel, Manonmani Swaminathan & Robin Sabariappan

  3. IRRI South Asia Breeding Hub, Hyderabad, 502 324, India

    Vishnu Varthini Nachimuthu

  4. International Crop Research Institute for Semi-Arid Tropics, Hyderabad, 502 324, India

    Ponnaiyah Govintharaj

  5. Acharya N.G. Ranga Agricultural University, Bapatla, 522101, India

    Shalini Tannidi

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  1. Balaji Aravindhan Pandian
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  2. John Joel
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  3. Vishnu Varthini Nachimuthu
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  4. Manonmani Swaminathan
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  5. Ponnaiyah Govintharaj
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  6. Shalini Tannidi
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  7. Robin Sabariappan
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Correspondence to Manonmani Swaminathan.

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Corresponding editor: Manoj Prasad

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Pandian, B.A., Joel, J., Nachimuthu, V.V. et al. Marker-aided selection and validation of various \({ Pi}\) gene combinations for rice blast resistance in elite rice variety ADT 43. J Genet 97, 945–952 (2018). https://doi.org/10.1007/s12041-018-0988-7

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  • DOI: https://doi.org/10.1007/s12041-018-0988-7

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