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Evaluation of Long Non-coding RNA Expression Profiles in Peripheral Blood Mononuclear Cells of Patients with Parkinson’s Disease

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Abstract

As in many biological processes, the long non-coding RNAs (lncRNA) are currently known to have important roles in Parkinson’s disease (PD). The aim of the study is to evaluate differentiated expressions of lncRNAs and their target mRNAs in the peripheral blood cells of individuals with Parkinson’s disease. The peripheral blood samples were taken from 10 Parkinson’s diagnosed people aging 50 years and more and from 10 healthy people as for the control group. Total RNA was isolated from peripheral blood mononuclear cells (PBMC), and a total of 5 samples were selected and evaluated by microarray analysis. lncRNAs with high fold change (fc < 1.5/fc > 1.5) were determined as a result of the analysis. Following this, the expression changes of some lncRNAs and their target mRNAs were examined by quantitative simultaneous polymerase chain reaction (qRT-PCR) in all individuals in the patient and control groups. Also, in order to determine the molecular level basic activities of lncRNAs determined by microarray analysis and which biological process and biochemical pathway they were in, Gene Ontology (GO) analysis (http://geneontology.org/) database was used. Thirteen upregulated and 31 downregulated lncRNAs whose expression changes were determined by microarray analysis and confirmed by qRT-PCR method were found in Parkinson’s patients. As they were evaluated by GO analysis, lncRNAs were expressed differently in patient and control groups and they are found to be related with the processes such as macromolecule metabolic processes, immune system, gene expression, cell activation, ATPase activity, DNA packaging complex, signal receptor activity, immune receptor activity, and protein binding were found to be significant.

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Data Availability

All the data generated and analyzed during this study are included in the published article.

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Acknowledgements

The authors thank Dr. Elif Avcı and Dr. Ahmet Mağrur Karagülmez in the Department of Neurology, Pamukkale University, for their diligent assistance with the data collection for this study.

Funding

This study is supported by Pamukkale University Scientific Research Projects Coordinatorship with the project number 2020SABE024.

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Authors and Affiliations

  1. Department of Medical Biology, Faculty of Medicine, Pamukkale University, Kınıklı/Denizli, Turkey

    Fatma Gizem Sarıekiz & Ayşe Gaye Tomatır

  2. Department of Medical Genetics, Faculty of Medicine, Pamukkale University, Denizli, Turkey

    Pervin Elvan Tokgün

  3. Department of Neurology, Faculty of Medicine, Pamukkale University, Denizli, Turkey

    Levent Sinan Bir

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  1. Fatma Gizem Sarıekiz
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  2. Ayşe Gaye Tomatır
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Contributions

F. Gizem Sarıekiz, A. Gaye Tomatir, and P. Elvan Tokgun designed the study. L. Sinan Bir selected the patients included in the study. F. Gizem sarıekiz and p. Elvan Tokgun performed the wet lab of the study. F. Gizem Sarıekiz, A. Gaye Tomatir, and P. Elvan Tokgun analyzed the data and wrote the manuscript. F. Gizem Sarıekiz, A. Gaye Tomatir, P. Elvan Tokgun, and L. Sinan Bir read and confirmed the study.

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Correspondence to Fatma Gizem Sarıekiz.

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Ethics Approval and Consent to Participate

All protocols used in this study were approved by the Ethics Commitee of Pamukkale University Hospital, Turkey. All procedures performed in this study involving human participants were in accordance with the ethical standards of the institutional commitee. Informed concent was obtained from all individual participants included in the study.

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The informed consent was obtained from study participants.

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The authors declare no competing interests.

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Sarıekiz, F.G., Tomatır, A.G., Tokgün, P.E. et al. Evaluation of Long Non-coding RNA Expression Profiles in Peripheral Blood Mononuclear Cells of Patients with Parkinson’s Disease. Mol Neurobiol 60, 6201–6211 (2023). https://doi.org/10.1007/s12035-023-03470-0

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  • DOI: https://doi.org/10.1007/s12035-023-03470-0

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