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Stable MSAP Markers for the Distinction of Vitis vinifera cv Pinot Noir Clones

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Abstract

Grapevine is one of the most economically important fruit crops. Molecular markers have been used to study grapevine diversity. For instance, simple sequence repeats are a powerful tool for identification of grapevine cultivars, while amplified fragment length polymorphisms have shown their usefulness in intra-varietal diversity studies. Other techniques such as sequence-specific amplified polymorphism are based on the presence of mobile elements in the genome, but their detection lies upon their activity. Relevant attention has been drawn toward epigenetic sources of variation. In this study, a set of Vitis vinifera cv Pinot noir clones were analyzed using the methylation-sensitive amplified polymorphism technique with isoschizomers MspI and HpaII. Nine out of fourteen selective primer combinations were informative and generated two types of polymorphic fragments which were categorized as “stable” and “unstable.” In total, 23 stable fragments were detected and they discriminated 92.5 % of the studied clones. Detected stable polymorphisms were either common to several clones, restricted to a few clones or unique to a single clone. The identification of these stable epigenetic markers will be useful in clonal diversity studies. We highlight the relevance of stable epigenetic variation in V. vinifera clones and analyze at which level these markers could be applicable for the development of forthright techniques for clonal distinction.

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Acknowledgments

The authors would like to thank Jean-Michel Menant (ATVB) and Francis Minet (Pepinières Guillaume) for kindly providing the plant material used in this study, Dr. Najoi El Azhari (Welience) for technical support with the Li-Cor sequencer, Franck Fickinger-Villemin for MSAP technical assistance and Dr. Marc Lollier for the statistical part of the manuscript. This study was funded by the Fonds Unique Interministériel (FUI) and OSEO including a PhD grant for J. O.

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  1. Laboratoire Vigne, Biotechnologies et Environnement, Université de Haute Alsace, 33 rue de Herrlisheim, 68008, Colmar, France

    Juan Ocaña, Bernard Walter & Paul Schellenbaum

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  1. Juan Ocaña
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  2. Bernard Walter
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  3. Paul Schellenbaum
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Correspondence to Paul Schellenbaum.

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Ocaña, J., Walter, B. & Schellenbaum, P. Stable MSAP Markers for the Distinction of Vitis vinifera cv Pinot Noir Clones. Mol Biotechnol 55, 236–248 (2013). https://doi.org/10.1007/s12033-013-9675-3

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