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Comparable Effects of Low-intensity Electromagnetic Irradiation at the Frequency of 51.8 and 53 GHz and Antibiotic Ceftazidime on Lactobacillus acidophilus Growth and Survival

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Abstract

The effects of low-intensity electromagnetic irradiation (EMI) with the frequencies of 51.8 and 53 GHz on Lactobacillus acidophilus growth and survival were revealed. These effects were compared with antibacterial effects of antibiotic ceftazidime. Decrease in bacterial growth rate by EMI was comparable with the inhibitory effect of ceftazidime (minimal inhibitory concentration—16 μM) and no enhanced action was observed with combined effects of EMI and the antibiotic. However, EMI-enhanced antibiotic inhibitory effect on bacterial survival. The kinetics of the bacterial suspension oxidation–reduction potential up to 24 h of the growth was changed by EMI and ceftazidime. The changes were more strongly expressed by combined effects of EMI and antibiotic especially up to 12 h. Moreover, EMI did not change overall energy (glucose)-dependent H+ efflux across the membrane but it increased N,N′-dicyclohexylcarbodiimide (DCCD)-inhibited H+ efflux. In contrast, this EMI in combination with ceftazidime decreased DCCD-sensitive H+ efflux. Low-intensity EMI had inhibitory effect on L. acidophilus bacterial growth and survival. The effect on bacterial survival was more significant in the combination with ceftazidime. The H+-translocating F 0 F 1-ATPase, for which DCCD is specific inhibitor, might be a target for EMI and ceftazidime. The revealed bactericide effects on L. acidophilus can be applied in biotechnology, food producing and safety technology.

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Acknowledgments

The authors thank to Dr. E. Afrikyan (Microbial Depository Center, National Academy of Sciences of Armenia, Abovyan City, Armenia) for strain and advice, Dr. V. Kalantaryan (Department of High Frequency Radiophysics and Telecommunication, Yerevan State University, Armenia) for a generator, Dr. V. Ohanyan for help to edit this manuscript, and H. Torgomyan in our lab for help in some measurements. The study was done in framework of basic support from Ministry of Education and Science of Armenia.

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The authors have no any conflict of interest.

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Authors and Affiliations

  1. Department of Biophysics, Biology Faculty, Yerevan State University, 1 A. Manoukian Str., 0025, Yerevan, Armenia

    Diana Soghomonyan

  2. Department of Microbiology, Plants and Microbes Biotechnology, Biology Faculty, Yerevan State University, 1 A. Manoukian Str., 0025, Yerevan, Armenia

    Armen Trchounian

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  1. Diana Soghomonyan
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  2. Armen Trchounian
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Correspondence to Armen Trchounian.

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Soghomonyan, D., Trchounian, A. Comparable Effects of Low-intensity Electromagnetic Irradiation at the Frequency of 51.8 and 53 GHz and Antibiotic Ceftazidime on Lactobacillus acidophilus Growth and Survival. Cell Biochem Biophys 67, 829–835 (2013). https://doi.org/10.1007/s12013-013-9571-2

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  • DOI: https://doi.org/10.1007/s12013-013-9571-2

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