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Investigations into the Correlation Properties of Membrane Electroporation-Induced Inward Currents: Prediction of Pore Formation

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Abstract

Membrane electroporation (MEP) induces a drastic change in membrane conductance and permeability. However, the underlying mechanisms by which MEP-induced currents (I MEP) are generated or resealed remain unclear. In this study, we investigated how the fluctuations of I MEP might be elicited in different types of cells, including pituitary GH3 cells, NG108-15 neuronal cells, and RAW 264.7 macrophages. We applied the detrended fluctuation analysis (DFA) to analyze the current signals in response to large hyperpolarizations. The DFA exponents from the current signals at 10 s preceding the start of the initial I MEP (I Pre) in GH3 cells exhibited two components (short time lag [α1] and long time lag [α2]) with a crossover threshold of about 7 ms. The α1 value was 0.46 ± 0.04 (n = 7), whereas the α2 value with 0.62 ± 0.05 (n = 7) indicated the presence of long-term correlations of current signals. However, during maximal I MEP, the slope of double logarithmic plot was linear and estimated to be 0.99 ± 0.02 (n = 8) with no clear crossover. Upon changes in membrane polarization, neither short- nor long-range correlation was altered. Chloroquine (CQ), a lysosomotropic agent, decreased the I MEP amplitude with an IC50 value of 46 μM; however, it had no effects on the scaling exponents of I Pre or I MEP. Although CQ or membrane polarization altered the amplitudes of I MEP, no changes in correlation properties of this current were detected. The scaling exponents derived from I Pre exhibit long-range correlations in these different types of cells, indicating there is a correlated character of the electropore dynamics that may be allowed to predict the MEP process.

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Abbreviations

BKCa channel:

Large-conductance Ca2+-activated K+ channel

CQ:

Chloroquine

DFA:

Detrended fluctuation analysis

DMEM:

Dulbecco’s modified Eagle’s medium

erg :

Ether-à-go-go-related-gene

IC50 :

The concentration required for 50% inhibition

I MEP :

Membrane electroporation-induced current

I Pre :

Current signal obtained at 10 s before the start of I MEP rise

MEP:

Membrane electroporation

SD:

Standard deviation

SEM:

Standard error of mean

SSR:

Sum of squared residuals

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Acknowledgments

The authors thank the National Science Council of Taiwan for financial support of this work under contracts (NSC-98-2320-B-006-027-MY3 and NSC-99-2918-I-006-003).

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Physiology, National Cheng Kung University Medical College, No. 1, University Road, Tainan City, 70101, Taiwan

    Sheng-Nan Wu, Chia-Chen Yeh, Pei-Yu Wu, Hsien-Ching Huang & Mei-Ling Tsai

  2. Institute of Basic Medical Sciences, National Cheng Kung University Medical College, No. 1, University Road, Tainan City, 70101, Taiwan

    Sheng-Nan Wu

  3. Department of Anatomy and Cell Biology, National Cheng Kung University Medical College, No. 1, University Road, Tainan City, 70101, Taiwan

    Sheng-Nan Wu

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Wu, SN., Yeh, CC., Wu, PY. et al. Investigations into the Correlation Properties of Membrane Electroporation-Induced Inward Currents: Prediction of Pore Formation. Cell Biochem Biophys 62, 211–220 (2012). https://doi.org/10.1007/s12013-011-9284-3

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