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Modeling the Kinetic Mechanisms of Voltage-Gated Ion Channels

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Advanced Patch-Clamp Analysis for Neuroscientists

Part of the book series: Neuromethods ((NM,volume 113))

Abstract

From neurons to networks, the kinetic properties of voltage-gated ion channels determine specific patterns of activity. In this chapter, we discuss how experimental data can be obtained and analyzed to formulate kinetic mechanisms and estimate parameters, and how these kinetic models can be tested in live neurons using dynamic clamp. First, we introduce the Markov formalism, as applied to modeling ion channel mechanisms, and the quantitative properties of single-channel and macroscopic currents obtained in voltage-clamp experiments. Then, we discuss how to design optimal voltage-clamp protocols and how to handle experimental artifacts. Next, we review the theoretical and practical aspects of data fitting, explaining how to define and calculate the goodness of fit, how to formulate model parameters and constraints, and how to search for optimal parameters. Finally, we discuss the technical requirements for dynamic-clamp experiments and illustrate the power of this experimental-computational approach with an example.

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Authors and Affiliations

  1. Division of Biological Sciences, University of Missouri, 205 Lefevre Hall, Columbia, MO, 65211, USA

    Autoosa Salari, Marco A. Navarro & Lorin S. Milescu

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  1. Autoosa Salari
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  2. Marco A. Navarro
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  3. Lorin S. Milescu
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Correspondence to Lorin S. Milescu .

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Editors and Affiliations

  1. The Leslie and Susan Gonda Multidisciplinary Brain Research Center, The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel

    Alon Korngreen

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Salari, A., Navarro, M.A., Milescu, L.S. (2016). Modeling the Kinetic Mechanisms of Voltage-Gated Ion Channels. In: Korngreen, A. (eds) Advanced Patch-Clamp Analysis for Neuroscientists. Neuromethods, vol 113. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3411-9_13

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  • DOI: https://doi.org/10.1007/978-1-4939-3411-9_13

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