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Recording and Hodgkin-Huxley Kinetic Analysis of Voltage-Gated Ion Channels in Nucleated Patches

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Advanced Patch-Clamp Analysis for Neuroscientists

Part of the book series: Neuromethods ((NM,volume 113))

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Abstract

The patch-clamp technique is widely used to measure and characterize ion currents flowing through the cell membrane. Various configurations can be used according to the research hypothesis. A relatively new configuration of the patch-clamp technique is the outside-out nucleated patch, which allows measuring somatic currents in voltage-clamp recordings due to a reduction in technical issues. Characterization of ionic currents is mostly based on the Hodgkin-Huxley model, a detailed mathematical and biophysical model of membrane excitability. This chapter describes voltage-clamp experiments step by step, from preparation and performing outside-out nucleated patch experiments through to the Hodgkin-Huxley analysis of the recorded data.

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Acknowledgments

This work was supported by a grant from the German-Israeli Foundation to AK (#1091-27.1/2010).

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Authors and Affiliations

  1. The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 52900, Israel

    Mara Almog & Alon Korngreen

  2. Leslie and Susan Gonda Multidisciplinary Brain Research Center, Bar-Ilan University, Ramat-Gan, 52900, Israel

    Mara Almog & Alon Korngreen

Authors
  1. Mara Almog
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  2. Alon Korngreen
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Corresponding author

Correspondence to Alon Korngreen .

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Editors and Affiliations

  1. The Leslie and Susan Gonda Multidisciplinary Brain Research Center, The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel

    Alon Korngreen

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Almog, M., Korngreen, A. (2016). Recording and Hodgkin-Huxley Kinetic Analysis of Voltage-Gated Ion Channels in Nucleated Patches. In: Korngreen, A. (eds) Advanced Patch-Clamp Analysis for Neuroscientists. Neuromethods, vol 113. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3411-9_14

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  • DOI: https://doi.org/10.1007/978-1-4939-3411-9_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3409-6

  • Online ISBN: 978-1-4939-3411-9

  • eBook Packages: Springer Protocols

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