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Screening of Selenium/Glutathione-Enriched Candida utilis and Its Anti-inflammatory and Antioxidant Activities in Mice

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Abstract

This study aimed to screen a mutant of Candida utilis SE-172 with high selenite tolerance and glutathione (GSH) biosynthesis capability via 60Co γ-radiation mutagenesis to prepare selenium (Se)-enriched yeast. The maximal intracellular contents of GSH and organic Se of 22.94 mg/g and 1308.1 μg/g were obtained, respectively, under a batch culture of SE-172. The physiological mechanism underlying increased GSH and organic Se contents in Se/GSH-enriched C. utilis SE-172 was revealed based on assaying activities of γ-glutamylcysteine synthase (γ-GCS) involved in GSH biosynthesis and selenophosphate synthase (SPS) related to organic Se bioconversion, and by determining intracellular ATP and NADH contents and ATP/ADP and NADH/NAD+ ratios associated with energy supply and regeneration. Moreover, the effect of this selenized yeast on anti-inflammatory and antioxidant activities in mice with colitis was investigated. The supplementation of Se/GSH-enriched yeast decreased the dextran sodium sulfate-induced damage to colon tissues, reduced the expression of pro-inflammatory factors [interleukin (IL)-6, IL-1β, and tumor necrosis factor-α (TNF-α)] in serum, increased the antioxidant-related enzyme [superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] activities, and decreased the malondialdehyde content in colon. The Se/GSH-enriched C. utilis SE-172 showed potent anti-inflammatory and antioxidant activities in mice with colitis.

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Funding

This work was supported by the National Natural Science Foundation of China (21506136, 21776189), and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Authors and Affiliations

  1. School of Biology and Basic Medical Sciences, Soochow University, 199# Ren’ai Road, Suzhou, 215123, People’s Republic of China

    Daohong He, Huasheng Wu, Huishu Jiang, Zhen Zhang, Chonglong Wang, Dahui Wang & Gongyuan Wei

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  1. Daohong He
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Contributions

DH designed the research, conducted animal experiments, analyzed the data, and drafted the initial version of the manuscript. HW and HJ performed lab experiments. ZZ performed lab experiments, analyzed the data. CW performed statistical analysis and validated the data. DW acquired funding, reviewed the manuscript and validated the data. GW conceived and designed research, administered project and acquired funding, edited the final version of the manuscript.

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Correspondence to Gongyuan Wei.

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All animal experimental operations were conducted according to the experimental animal management regulations of Soochow University (SZUM2008031233).

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He, D., Wu, H., Jiang, H. et al. Screening of Selenium/Glutathione-Enriched Candida utilis and Its Anti-inflammatory and Antioxidant Activities in Mice. Biol Trace Elem Res 202, 2786–2796 (2024). https://doi.org/10.1007/s12011-023-03882-y

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