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Yeast Selenium Exerts an Antioxidant Effect by Regulating the Level of Selenoprotein to Antagonize Cd-Induced Pyroptosis of Chicken Liver

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Abstract

Cadmium (Cd) exposure can cause multiple organ damage in humans and animals by causing oxidative stress. Organic selenium can antagonize the toxic damage of heavy metals by exerting antioxidant effects. Oxidative stress can activate NLRP3/Caspase1-dependent pyroptosis, but it is not clear whether yeast selenium (Se Y) can antagonize Cd-induced pyroptosis in the chicken liver. In this experiment, we studied the effects of CdCl2 single, Se Y single, and combined exposure on the pyroptosis of chicken liver. The results showed that Cd exposure induced oxidative stress in the chicken liver, and the expression of Sepx1, SelU, SelT, GPx1, GPx4, Dio1, Dio2, Dio3, TrxR1, TrxR2, TrxR3, SelH, SelI, SelO, SelK, SelPb, Selpp, and Sel15 decreased. In addition, NLRP3 inflammasome complex (NOD-like receptor protein 3 (NLRP3), apoptosis-associated speck-like protein containing (ASC), and pro-Caspase1), Cysteinyl aspartate specific proteinase (Caspase1), Interleukin-β (IL-1β), and Interleukin-18 (IL-18) were upregulated. The combined treatment of Se Y and Cd found that the antioxidant level and the expression level of selenoprotein recovered to the Se group, and the liver pyroptosis decreased. Principal component analysis and correlation analysis screened out that selenoprotein O (SelO) is negatively correlated with pyroptosis-related genes. In short, our data shows that Se Y exerts an antioxidant effect by increasing the expression of selenoproteins and antagonizing the pyroptosis of chicken liver induced by Cd. This study provides new ideas for the field of heavy metal toxicology research. And the selected SelO has an important reference significance for follow-up research.

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Availability of Data and Materials

The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.

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Acknowledgements

The authors extend their sincere thanks to the members of the veterinary internal medicine laboratory and key Laboratory for Laboratory Animals and Comparative Medicine at the College of Veterinary Medicine, Northeast Agricultural University.

Funding

This study was supported by the National Natural Science Foundation of China Youth Foundation (31602028) and the Postdoctoral Foundation of Heilongjiang Province (Project No. LBHQ19068).

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Authors and Affiliations

  1. College of International Culture and Education, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Xing Pengcheng

  2. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Shi Xu, Cui Wei & Huang Xiaodan

Authors
  1. Xing Pengcheng
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  2. Shi Xu
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  3. Cui Wei
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  4. Huang Xiaodan
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Contributions

Xing Pengcheng: visualization, investigation, writing-original draft. Shi Xu: manuscript revision, formal analysis. Cui Wei: software, investigation. Huang Xiaodan: conceptualization, resources, supervision, validation, writing-review and editing.

Corresponding author

Correspondence to Huang Xiaodan.

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All procedures used in this study were approved by the Institutional Animal Care and Use Committee of Northeast Agricultural University (SRM-11). All authors have read and approved the paper, and the paper has not been published before and has not been considered by any other peer-reviewed journal.

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Pengcheng, X., Xu, S., Wei, C. et al. Yeast Selenium Exerts an Antioxidant Effect by Regulating the Level of Selenoprotein to Antagonize Cd-Induced Pyroptosis of Chicken Liver. Biol Trace Elem Res 200, 4079–4088 (2022). https://doi.org/10.1007/s12011-021-02984-9

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  • DOI: https://doi.org/10.1007/s12011-021-02984-9

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