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Selenomethionine relieves inflammation in the chicken trachea caused by LPS though inhibiting the NF-κB pathway

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Abstract

Selenomethionine is able to relieve the effect of inflammation in various tissues and organs. However, there are few studies about the influences of organic selenium resisting inflammation induced by LPS in chicken trachea. Therefore, the purpose of this experiment is to explore the organic selenium (selenomethionine) can raise immune function and relieve the LPS-induced inflammation of chicken trachea via inhibiting the NF-κB pathway. To investigate the mechanism of organic selenium on chicken trachea, the supplement of selenomethionine and/or LPS-induced chicken models were established. One hundred 46-week-old isa chickens were randomly divided into four groups (n = 25). The four groups were the control group, the selenomethionine group (Se group), the LPS-induced group (LPS group), and the Se and LPS interaction group (Se + LPS group). Then, the expressions of inflammatory factors (including induced nitric oxide synthase (iNOS), nuclear factor-kappa B(NF-κB), tumor necrosis factor (TNF-α), cyclooxygenase-2 (COX-2), and prostaglandin E (PTGEs) synthase), inflammation-related cytokines (including interleukin (IL-2, IL-6, IL-8, IL-17) and immunoglobulin (IgA, IgM, IgY)), the marker of immune function (avian β-defensins (AvBD6, AvBD7)), heat shock proteins (including HSP60, HSP90), and selenoproteins (including Selo, Sels, Selm, Selh, Selu, Seli, SPS2, GPx1, GPx2, Dio1, Sepx1, Sep15, Sepp1, Txnrd1) were detected in our experiment. The above genes were significantly changed in different groups (p < 0.05). We can conclude that organic selenium can increase the function of immunity and the expression of selenoproteins, and mitigate the inflammation induced by LPS via suppression of the NF-κB pathway.

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Acknowledgments

The authors extend their sincere thanks to the members of the veterinary internal medicine laboratory and key Laboratory for Laboratory Animals at the College of Veterinary Medicine, Northeast Agricultural University.

Conflict of Interest

The authors declare that they have no conflict of interest.

Funding

The study was supported by the National Key R&D Program of China (No. 2017YFD0502200).

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Authors and Affiliations

  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Xu Shi, Wei Wang, Shufang Zheng, Qiaojian Zhang & Shiwen Xu

  2. Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Shiwen Xu

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Shi, X., Wang, W., Zheng, S. et al. Selenomethionine relieves inflammation in the chicken trachea caused by LPS though inhibiting the NF-κB pathway. Biol Trace Elem Res 194, 525–535 (2020). https://doi.org/10.1007/s12011-019-01789-1

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