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Selenomethionine Alleviates Intestinal Ischemia–Reperfusion Injury in Mice Through the Bax-Caspase Pathway

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Abstract

Selenomethionine (SeMet) is known to alleviate ischemia–reperfusion (I/R) injury. However, its details of action have not been thoroughly elucidated in mice with intestinal I/R injury. In this study, intestinal I/R injury mice models were established, and ELISAs were performed to determine the levels of redox factors, including glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA), in mice intestinal tissues. Furthermore, several apoptosis-related markers, such as cytochrome c (Cyt-c), Bcl-2, and Bax, were detected using qPCR and Western blotting, while caspase-3 was detected using Western blotting alone. The results showed that SeMet alleviated I/R damage by increasing GSH-Px, CAT, and SOD levels and reducing MDA levels. Our data demonstrated that SeMet reduced I/R injury and inhibited the expression of Cyt-c, Bax, and caspase-3. SeMet also increased the expression of Bcl-2 in the intestinal tissues of mice. In addition, the TUNEL assay results showed that SeMet mitigated apoptosis in the villi cells of the intestinal mucosa. The findings also revealed that I/R could lead to increased apoptosis levels and that SeMet alleviated I/R-induced apoptosis by mediating the Bax/cytochrome C/caspase-3 apoptotic signaling pathways in the intestinal I/R injury mice models. Thus, SeMet inhibited apoptosis and resulted in an increase of Bcl-2 levels; downregulated the expression of Bax, Cyt-c, and caspase-3; and alleviated the intestinal ischemia injury in mice. The I/R injury increased the cytosolic Bax, Cyt-c, and caspase-3 levels and significantly decreased Bcl-2 expression levels in the I/R group, compared to the Sham group. However, the levels of all markers were reversed post-SeMet pre-treatment.

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Data Availability

Data has been shared in the submitted article. Some data supporting the findings of this study will be availed upon request from the corresponding author. Some data are, however, are not publicly available due to privacy or ethical restrictions.

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Acknowledgements

The authors wish to thank the study participants and Jilin University for their contributions to this study.

Funding

This work was supported by grants from the National key research and development program (2016YFD0501203), Foundation of National Natural Science Project, P.R. China (31472249;31772789), Foundation Project of Heilongjiang province natural (No. H2017038), Heilongjiang Bayi Agricultural University Support Program for “San Zong” (TDJH201905), and Heilongjiang Bayi Agricultural University Support Program of “San Heng San Zong” (ZRCPY201909).

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Authors and Affiliations

  1. College of Life Sciences and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, China

    Zhe Liu, Zhiming Liang, Rui Zhao & Chenghao Jin

  2. National Coarse Cereals Engineering Research Center, Daqing, 163319, China

    Zhe Liu & Chenghao Jin

  3. The First Hospital of Qiqihar, Qiqihar, Heilongjiang, 161005, People’s Republic of China

    Guangze Mou

  4. Department of Food Science and Engineering, College of Food Science & Technology, Heilongjiang Bayi Agricultural University, Daqing, 163319, China

    Chenghao Jin

  5. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Tech Industrial Development Zone, Daqing, Heilongjiang, 163319, People’s Republic of China

    Rui Wu

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  1. Zhe Liu
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Contributions

The authors have read and approved the final manuscript. Zhe Liu and Guangze Mou conceptualized the manuscript. Guangze Mou and Rui Zhao conducted the statistical analyses. Zhe Liu and Zhiming Liang generated Figs. 2 and 6. Guangze Mou generated Fig. 5. Zhiming Liang provided data and developed Table 1. Chenghao Jin revised the paper and generated Fig. 3. Rui Wu developed Figs. 1 and 4.

Corresponding authors

Correspondence to Chenghao Jin or Rui Wu.

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Animal Ethics statement: All experimental procedures were approved by the Management Committee of the Experimental Animal Center of Heilongjiang Bayi Agricultural University (License No.22–05-01). The authors agreed to participate.

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The contribution of Zhe Liu and GuangZe Mou co-together is equally important in this article.

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Liu, Z., Mou, G., Liang, Z. et al. Selenomethionine Alleviates Intestinal Ischemia–Reperfusion Injury in Mice Through the Bax-Caspase Pathway. Biol Trace Elem Res 200, 3205–3214 (2022). https://doi.org/10.1007/s12011-021-02925-6

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