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Selenium protects the immature rat heart against ischemia/reperfusion injury

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Abstract

The aim of the study was to find out whether administration of selenium (Se) will protect the immature heart against ischemia/reperfusion.

The control pregnant rats were fed laboratory diet (0.237 mg Se/kg diet); experimental rats received 2 ppm Na2SeO3 in the drinking water from the first day of pregnancy until day 10 post partum. The concentration of Se in the serum and heart tissue was determined by activation analysis, the serum concentration of NO by chemiluminescence, cardiac concentration of lipofuscin-like pigment by fluorescence analysis. The 10 day-old hearts were perfused (Langendorff); recovery of developed force (DF) was measured after 40 min of global ischemia. In acute experiments, 10 day-old hearts were perfused with selenium (75 nmol/l) before or after global ischemia. Sensitivity to isoproterenol (ISO, pD50) was assessed as a response of DF to increasing cumulative dose.

Se supplementation elevated serum concentration of Se by 16%. Se increased ischemic tolerance (recovery of DF, 32.28 ± 2.37 vs. 41.82 ± 2.91%, P < 0.05). Similar results were obtained after acute administration of Se during post-ischemic reperfusion (32.28 ± 2.37 vs. 49.73 ± 4.40%, P < 0.01). The pre-ischemic treatment, however, attenuated the recovery (23.08 ± 3.04 vs. 32.28 ± 2.37%, P < 0.05). Moreover, Se supplementation increased the sensitivity to the inotropic effect of ISO, decreased cardiac concentration of lipofuscin-like pigment and serum concentration of NO.

Our results suggest that Se protects the immature heart against ischemia/reperfusion injury. It seems therefore, that ROS may affect the function of the neonatal heart, similarly as in adults.

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Abbreviations

Se:

Selenium

ROS:

Radical oxygen species

I/R:

Ischemia reperfusion

DF:

Developed force

ISO:

Isoproterenol

NF-κB:

Nuclear factor-κB

cGMP:

Cyclic guanosine monophosphate

iNOS:

Inducible nitric oxide synthase

LFP:

Lipofuscin-like pigment

rfu:

Relative fluorescence unit

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Acknowledgements

The authors thank Mrs. M. Peskova for technical assistance.

This work was supported by grant No. 1M0510 of the Czech Ministry of Education, Youth and Physical Education, by grant No. AV 0Z 50110509 and by grant AV 0Z 40310501.

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Authors and Affiliations

  1. Centre of cardiovascular research, Institute of Physiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague 4–Krc, Czech Republic

    Ivana Ostadalova, Zuzana Chvojkova & Bohuslav Ostadal

  2. Institute of Analytical Chemistry, Department of Trace Element Analysis, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague 4–Krc, Czech Republic

    Miloslav Vobecky

  3. Department of Physiology, Charles University Second Medical School, Prague, Czech Republic

    Dana Mikova & Vaclav Hampl

  4. Department of Medical Chemistry and Biochemistry, Charles University Second Medical School, Prague, Czech Republic

    Jiri Wilhelm

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  1. Ivana Ostadalova
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Correspondence to Ivana Ostadalova.

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Ostadalova, I., Vobecky, M., Chvojkova, Z. et al. Selenium protects the immature rat heart against ischemia/reperfusion injury. Mol Cell Biochem 300, 259–267 (2007). https://doi.org/10.1007/s11010-006-9391-4

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  • DOI: https://doi.org/10.1007/s11010-006-9391-4

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