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Selenium Deficiency Induces Autophagy in Chicken Bursa of Fabricius Through ChTLR4/MyD88/NF-κB Pathway

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Abstract

To explore the role of ChTLR4/MyD88/NF-κB signaling pathway on autophagy induced by selenium (Se) deficiency in the chicken bursa of Fabricius, autophagosome formation in the bursa of Fabricius was observed by transmission electron microscopy. Quantitative real-time PCR (qRT-PCR) and Western blot were used to detect the expression of ChTLR4 and its signaling pathway molecules (MyD88, TRIF, and NF-κB), inflammatory factors (IL-1β, IL-8, and TNF-α), and autophagy-related factors (ATG5, Beclin1, and LC3-II) in the Se-deficient chicken bursa of Fabricius at different ages. The results showed that ChTLR4/MyD88/NF-κB signaling pathway was activated in the chicken bursa of Fabricius and autophagy was induced at the same time by Se deficiency. In order to verify the relationship between the autophagy and ChTLR4/MyD88/NF-κB signaling pathway, HD11 cells were used to establish the normal C group, low Se group, and low Se + TLR4 inhibitor (TAK242) group. The results demonstrated that autophagy could be hindered when the TLR4 signaling pathway was inhibited under Se deficiency. Furthermore, autophagy double-labeled adenovirus was utilized to verify the integrity of autophagy flow induced by Se deficiency in HD11 cells. The results showed that it appeared to form a complete autophagy flow under the condition of Se deficiency and could be blocked by TAK242. In summary, we found that Se deficiency was involved in the chicken bursa of Fabricius autophagy occurring by activating the ChTLR4/MyD88/NF-κB pathway.

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Data Availability Statement

The data used to support the findings of this study are available from the corresponding author upon request.

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Acknowledgements

We thank the Laboratory of Anatomic Pathology at the College of Veterinary Medicine, Northeast Agricultural University. All of the authors read the manuscript and agreed to submit it in its current form for consideration for publication.

Funding

This study was supported by the National Natural Science Foundation of China Youth Foundation (31602028) and the Postdoctoral Foundation of Heilongjiang Province (Project No. LBHQ19068).

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Author notes

  1. Ruili Zhang and Qing Liu contributed equally to this work and should be considered co-first authors.

Authors and Affiliations

  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Ruili Zhang, Qing Liu, Rong Guo, Di Zhang, Yang Chen, Guangxing Li & Xiaodan Huang

  2. Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Harbin, 150030, People’s Republic of China

    Ruili Zhang, Qing Liu, Rong Guo, Di Zhang, Yang Chen, Guangxing Li & Xiaodan Huang

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  1. Ruili Zhang
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Contributions

Xiaodan Huang and Ruili Zhang conceived and designed the experiments. Qing Liu, Di Zhang Zhang Di, Rong Guo, and Yang Chen performed the experiments. Qing Liu and Di Zhang performed the cell culture. Qing Liu analyzed the data and wrote the paper. Ruili Zhang, Guangxing Li, and Xiaodan Huang assisted in correcting the manuscript.

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Correspondence to Xiaodan Huang.

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The Institution of Animal Protection and Utilization Committee at Northeast Agricultural University approved this experiment.

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All of the procedures used in this study were approved by the Institutional Animal Care and Use Committee of Northeast Agricultural University.

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Zhang, R., Liu, Q., Guo, R. et al. Selenium Deficiency Induces Autophagy in Chicken Bursa of Fabricius Through ChTLR4/MyD88/NF-κB Pathway. Biol Trace Elem Res 200, 3303–3314 (2022). https://doi.org/10.1007/s12011-021-02904-x

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