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Impact of Selenium Deficiency on Inflammation, Oxidative Stress, and Phagocytosis in Mouse Macrophages

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Abstract

Although it has been reported that selenium (Se) deficiency can trigger inflammation, however, there are few reports on the effect of Se on the function of mouse peritoneal macrophages. Herein, we examined the expression of inflammatory factors, oxidative stress levels, and phagocytosis for primary-cultured peritoneal macrophages using control and Se-deficient groups. Our results revealed that Se deficiency induced the accumulation of oxygen free radicals and weakened antioxidant capacity. Se deficiency also significantly increased the expression of inflammation factors including iNOS, IL-1β, IL-12, IL-10, PTGe, and NF-κB. Meanwhile, Se suppression restrained macrophage production of TNF-α. The results of the phagocytosis assay demonstrated that Se deficiency inhibited the phagocytosis of macrophages. In conclusion, Se-deficient macrophages undergo severe inflammation through the NF-κB pathway due to the accumulation of oxygen free radicals and are hindered in their phagocytic capacity.

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Abbreviations

GSH-Px:

glutathione peroxidase

CAT:

catalase

MDA:

malondialdehyde

iNOS:

induced nitric oxide synthase

SOD:

superoxide dismutase

T-AOC:

total antioxidant capability

TNF-α:

tumor necrosis factor

PTGEs:

prostaglandin e synthetase

iNOS:

inducible nitric oxide synthase

NF-κB:

nuclear factor kappa-light-chain-enhancer of activated B cells

IL-1β:

interleukin-1β

IL-10:

interleukin-10

IL-12:

interleukin-12

GAPDH:

glyceraldehyde-3-phosphate dehydrogenase

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Funding

The study was supported by the Earmarked Fund for China Agriculture Research System (No. CARS 35) and the Students Innovative Training (SIPT) program of Northeast Agricultural University (No. 201810224213).

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Authors and Affiliations

  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Jianwen Xu, Yafan Gong, Yue Sun, Jingzeng Cai, Qi Liu, Jie Yang & Ziwei Zhang

  2. College of Animal Science, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Jun Bao

  3. Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Ziwei Zhang

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  1. Jianwen Xu
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Correspondence to Jie Yang or Ziwei Zhang.

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All procedures used in this study were approved by the Institutional Animal Care and Use Committee of Northeast Agricultural University (SRM-11).

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Xu, J., Gong, Y., Sun, Y. et al. Impact of Selenium Deficiency on Inflammation, Oxidative Stress, and Phagocytosis in Mouse Macrophages. Biol Trace Elem Res 194, 237–243 (2020). https://doi.org/10.1007/s12011-019-01775-7

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