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Effects of Nickel Chloride on the Erythrocytes and Erythrocyte Immune Adherence Function in Broilers

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Abstract

This study was conducted to investigate the immune adherence function of erythrocytes and erythrocyte induced by dietary nickel chloride (NiCl2) in broilers fed on a control diet and three experimental diets supplemented with 300, 600, and 900 mg/kg NiCl2 for 42 days. Blood samples were collected from five broilers in each group at 14, 28, and 42 days of age. Changes of erythrocyte parameters showed that total erythrocyte count (TEC), hemoglobin (Hb) contents, and packed cell volume (PCV) were significantly lower (p < 0.05 or p < 0.01) and erythrocyte osmotic fragility (EOF) was higher (p < 0.05 or p < 0.01) in the 600 and 900 mg/kg groups at 28 and 42 days of age than those in the control group, and the sodium-potassium adenosine triphosphatase (Na+/K+-ATPase) and calcium adenosine triphosphatase (Ca2+-ATPase) activities were significantly decreased (p < 0.05 or p < 0.01) in the NiCl2-treated groups. The results of erythrocyte immune adherence function indicated that erythrocyte C3b receptor rosette rate (E-C3bRR) was significantly decreased (p < 0.05 or p < 0.01) in the 600 and 900 mg/kg groups and in the 300 mg/kg group at 42 days of age, whereas the erythrocyte immune complex rosette rate (E-ICRR) was markedly increased (p < 0.05 or p < 0.01) in the 300, 600, and 900 mg/kg groups at 28 and 42 days of age. It was concluded that dietary NiCl2 in excess of 300 mg/kg caused anemia and impaired the erythrocytic integrity, erythrocytic ability to transport oxygen, and erythrocyte immune adherence function in broilers. Impairment of the erythrocytes and erythrocyte immune adherence function was one of main effect mechanisms of NiCl2 on the blood function.

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Acknowledgments

This research was supported by the program for Changjiang scholars and the University Innovative Research Team (IRT 0848), and the Shuangzhi project of Sichuan Agricultural University (03570327)

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The authors declare no conflict of interest.

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Authors and Affiliations

  1. Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Sichuan Agricultural University, 625014, Ya’an, China

    Jian Li, Bangyuan Wu, Hengmin Cui, Xi Peng, Jing Fang, Zhicai Zuo, Junliang Deng, Xun Wang, Kun Tang & Shuang Yin

  2. College of Veterinary Medicine, Sichuan Agricultural University, 625014, Ya’an, China

    Hengmin Cui, Xi Peng, Jing Fang, Zhicai Zuo, Junliang Deng & Xun Wang

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  1. Jian Li
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  2. Bangyuan Wu
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Correspondence to Hengmin Cui.

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Li, J., Wu, B., Cui, H. et al. Effects of Nickel Chloride on the Erythrocytes and Erythrocyte Immune Adherence Function in Broilers. Biol Trace Elem Res 161, 173–179 (2014). https://doi.org/10.1007/s12011-014-0096-3

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