Abstract
This study was designed to evaluate the effects of dietary nickel chloride (NiCl2) on the splenic immunity in broilers by observing changes of cytokine mRNA expression and protein levels, immunoglobulin (IgA, IgG, and IgM) contents, and IgA+ B cell and T-cell numbers using the methods of qRT-PCR, flow cytometry (FCM), and ELISA. A total of 240 1-day-old avian broilers were equally allocated into four groups and fed on a corn–soybean basal diet as the control diet or the same diet supplemented with 300, 600, and 900 mg/kg NiCl2 for 42 days. The mRNA expression and protein levels of IL-2, IL-6, IL-10, IL-12, TNF-α/LITAF, IFN-γ, and IgA, IgG, and IgM contents were significantly decreased (p < 0.05 or p < 0.01) in the 300-, 600-, and 900-mg/kg NiCl2 groups when compared with those of the control group, which was consistent with the reduction of T-cell subset percentages and IgA+ B cell numbers in the 300-, 600-, and 900-mg/kg NiCl2 groups. The abovementioned results showed that dietary NiCl2 in excess of 300 mg/kg caused damage on splenocytes and splenic immune function. The results of the present study provided new experimental evidences for further study on the effect mechanism of NiCl2 on splenic immunity.
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References
Storey M, Jordan S (2008) An overview of the immune system. Nurs Stand 23(15):47–56
Parkin J, Cohen B (2001) An overview of the immune system. Lancet 357(9270):1777–1789
Mebius RE, Kraal G (2005) Structure and function of the spleen. Nat Rev Immunol 5(8):606–616
Cempel M, Nikel G (2006) Nickel: a review of its sources and environmental toxicology. Pol J Environ Stud 15(3):375–382
Samal L, Mishra C (2011) Significance of nickel in livestock health and production. International Journal for Agro Veterinary and Medical Sciences 5(3):349–361
Kasprzak KS, Sunderman FW Jr, Salnikow K (2003) Nickel carcinogenesis. Mutat Res Fundam Mol Mech Mutagen 533(1):67–97
Doreswamy K, Shrilatha B, Rajeshkumar T (2004) Nickel-induced oxidative stress in testis of mice: evidence of DNA damage and genotoxic effects. J Androl 25(6):996–1003
Donskoy E, Donskoy M, Forouhar F, Gillies C, Marzouk A, Reid M, Zaharia O, Sunderman F (1986) Hepatic toxicity of nickel chloride in rats. Annals ClinLab Sci 16(2):108–117
Haley PJ, Bice DE, Muggenburg BA, Hann FF, Benjamin SA (1987) Immunopathologic effects of nickel subsulfide on the primate pulmonary immune system. Toxicol Appl Pharmacol 88(1):1–12
Graham JA, Miller FJ, Daniels MJ, Payne EA, Gardner DE (1978) Influence of cadmium, nickel, and chromium on primary immunity in mice. Environ Res 16(1):77–87
Smialowicz RJ, Rogers RR, Rowe DG, Riddle MM, Luebke RW (1987) The effects of nickel on immune function in the rat. Toxicology 44(3):271–281
Schiffer RB, Sunderman W Jr, Baggs RB, Moynihan JA (1991) The effects of exposure to dietary nickel and zinc upon humoral and cellular immunity in SJL mice. J Neuroimmunol 34(2):229–239
Graham JA, Gardner DE, Miller FJ, Daniels MJ, Coffin DL (1975) Effect of nickel chloride on primary antibody production in the spleen. Environ Health Perspect 12:109–113
Ling J, Leach R (1979) Studies on nickel metabolism: interaction with other mineral elements. Poult Sci 58(3):591–596
Kremling K (1985) The distribution of cadmium, copper, nickel, manganese, and aluminium in surface waters of the open Atlantic and European shelf area. Deep Sea Res A Oceanographic Res Pap 32(5):531–555
Yu Y, Qiu L, Song L, Zhao J, Ni D, Zhang Y, Xu W (2007) Molecular cloning and characterization of a putative lipopolysaccharide-induced TNF-α factor (LITAF) gene homologue from Zhikong scallop Chlamys farreri. Fish Shellfish Immunol 23(2):419–429
National Research Council (US) (1994) Nutrient requirements of poultry, 9th revised edn. National Academy, Washington DC
Chen K, Shu G, Peng X, Fang J, Cui H, Chen J, Wang F, Chen Z, Zuo Z, Deng J (2013) Protective role of sodium selenite on histopathological lesions, decreased T-cell subsets and increased apoptosis of thymus in broilers intoxicated with aflatoxin B1. Food Chem Toxicol 59:446–454
Shirkey RJ, Chakraborty J, Bridges JW (1979) An improved method for preparing rat small intestine microsomal fractions for studying drug metabolism. Anal Biochem 93:73–81
Gaça MD, Pickering JA, Arthur MJ, Benyon RC (1999) Human and rat hepatic stellate cells produce stem cell factor: a possible mechanism for mast cell recruitment in liver fibrosis. J Hepatol 30(5):850–858
Luo Q, Cui H, Peng X, Fang J, Zuo Z, Deng J, Liu J, Deng Y (2013) Intestinal IgA + cell numbers as well as IgA, IgG, and IgM contents correlate with mucosal humoral immunity of broilers during supplementation with high fluorine in the diets. Biol Trace Elem Res 154(1):62–72
Stiehm ER, Wakim M (1997) The spleen in infection and immunity. In: Hiatt J, Phillips E, Morgenstern L (eds) Surgical diseases of the spleen. Springer, Berlin, pp 53–59
Tiron A, Vasilescu C (2007) Role of the spleen in immunity. Immunologic consequences of splenectomy. Chirurgia 103(3):255–263 (Bucharest, Romania: 1990)
Swirski FK, Nahrendorf M, Etzrodt M, Wildgruber M, Cortez-Retamozo V, Panizzi P, Figueiredo J-L, Kohler RH, Chudnovskiy A, Waterman P (2009) Identification of splenic reservoir monocytes and their deployment to inflammatory sites. Science 325(5940):612–616
Jia T, Pamer EG (2009) Immunology: dispensable but not irrelevant. Science (New York, NY) 325(5940):549–550
Hecht GA (2003) Microbial pathogenesis and the intestinal epithelial cell. ASM, Washington, DC
Feldmann M (1998) Cytokines. In: Peter JD (ed) Encyclopedia of immunology, 2nd edn. Elsevier, Oxford, pp 719–722
Abreu-Martin MT, Targan SR (1996) Regulation of immune responses of the intestinal mucosa. Crit Rev™ in Immunol 16(3):277–309
Cui W, Cui H, Peng X, Zuo Z, Liu X, Wu B (2011) Effect of vanadium on the subset and proliferation of peripheral blood T cells, and serum interleukin-2 content in broilers. Biol Trace Elem Res 141(1–3):192–199
Kannangai R, Prakash KJ, Ramalingam S, Abraham OC, Mathews KP, Jesudason MV, Sridharan G (2000) Peripheral CD4+/CD8+ T-lymphocyte counts estimated by an immunocapture method in the normal healthy South Indian adults and HIV seropositive individuals. J Clin Virol 17(2):101–108
Hernández J, Garfias Y, Nieto A, Mercado C, Montaño LF, Zenteno E (2001) Comparative evaluation of the CD4 + CD8+ and CD4 + CD8− lymphocytes in the immune response to porcine rubulavirus. Vet Immunol Immunopathol 79(3):249–259
Kotb MA, El Henawy A, Talaat S, Aziz M, El Tagy GH, EI Barbary MM, Mostafa W (2005) Immune-mediated liver injury: prognostic value of CD4+, CD8+, and CD68+ in infants with extrahepatic biliary atresia. J Pediatr Surg 40(8):1252–1257
Chaplin DD (2006) Overview of the human immune response. J Allergy Clin Immunol 117(2):S430–S435
Wu B, Cui H, Peng X, Fang J, Zuo Z, Huang J, Luo Q, Deng Y, Wang H, Liu J (2013) Changes of the serum cytokine contents in broilers fed on diets supplemented with nickel chloride. Biol Trace Elem Res 151(2):234–239
Jolles S, Kaveri S, Orange J (2009) Current understanding and future directions. Clin Exp Immunol 158(s1):68–70
Perdigon G, Alvarez S, Rachid M, Agüero G, Gobbato N (1995) Immune system stimulation by probiotics. J Dairy Sci 78(7):1597–1606
Bonilla FA, Oettgen HC (2010) Adaptive immunity. J Allergy Clin Immunol 125(2):S33–S40
Acknowledgments
The study was supported by the Program for Changjiang Scholars and Innovative Research Team in University (IRT 0848) and the Education Department (09ZZ017) and Scientific Department of Sichuan Province.
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Huang, J., Cui, H., Peng, X. et al. Effect of Dietary Nickel Chloride on Splenic Immune Function in Broilers. Biol Trace Elem Res 159, 183–191 (2014). https://doi.org/10.1007/s12011-014-0003-y
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DOI: https://doi.org/10.1007/s12011-014-0003-y