Abstract
Proton Induced X-ray emission (PIXE) and fluorescence-activated cell sorting (FACS) have been used to study the effects of gamma irradiation on heavy metal accumulation in callus tissue of Plantago ovata—an important cash crop of India. PIXE analysis revealed radiation-induced alteration in trace element profile during developmental stages of the callus of P. ovata. Subsequent experiments showed antagonism between Fe and Cu and also Cu and Zn and synergistic effect between Fe and Zn. FACS analysis showed significant induction of the metallothionein (MT) protein following gamma-irradiation, and maximum induction was noted at the 50-Gy absorbed dose. This indicated a progressive increment of MTs as a measure for protection against gamma-rays, to combat alteration in the homeostasis of heavy metals like Fe, Cu, Zn, and Mn.
Similar content being viewed by others
References
Blinstrubiene A, Sliesaravicius A, Burbulis N (2004) Factors affecting morphogenesis in tissue culture of linseed flax (Linum usitatissimum L.). Acta Univ Latviensis Biol 676:149–152
Kothari SL, Agarwal K, Kumar S (2004) Inorganic nutrient manipulation for highly improved in vitro plant regeneration in finger millet—Eleusine coracana (L.) Gaertin. In Vitro Cell Dev Biol Plant 40:515–519
Niedz RP, Evens TJ (2007) Regulating plant growth by mineral nutrition. In Vitro Cell Dev Biol Plant 43:370–381
Wi SG, Chung BY, Kim JS, Kim JH, Baek MH, Lee JW, Kim YS (2007) Effects of gamma irradiation on morphological changes and biological responses in plants. Micron 38:553–564
Pramanik S, Chakraborty S, Sen Raychaudhuri S (1996) Changes in isozymes during in vitro morphogenesis in Plantago ovata Forsk. Plant Cell Tissue Org Cult 44:123–127
Das Pal M, Sen Raychaudhuri S (2000) Enhanced development of somatic embryos of Plantago ovata Forsk by additives. In Vitro Cell Dev Biol Plant 37:568–571
Sahasrabudhe NA, Nandi M, Bahulikar RA (1999) Influence of boric acid on somatic embryogenesis of a cytosterile line of indica rice. Plant Cell Tissue Org Cult 58:73–75
Desai NS, Joseph D, Suprasanna P, Bapat VA (2006) Study of elemental variations during somatic embryogenesis in sugarcane using photon induced X-ray probe. Nucl Instrum Methods B 252:299–302
Caselles J, Colliga C, Zornoza P (2002) Evaluation of trace element pollution from vehicle emission in petunia plants. Water Air Soil Pollut 136:1–9
Stenstrom A, Jonsdottir IS, Augner M (2002) Genetic and environmental effects on morphology in clonal sedges in the Eurasian Arctic. Am J Bot 89:1410–1421
Chen F, Vallyathan V, Castranova V, Shi X (2001) Cell apoptosis induced by carcinogenic metals. Mol Cell Biochem 222:183–188
Speranza A, Ferri P, Battistelli M, Falcieri E, Crinelli R, Scoccianti V (2007) Both trivalent and hexavalent chromium strongly alter in vitro germination and ultrastructure of kiwifruit pollen. Chemosphere 66:1165–1174
Poschenrieder C, Tolra R, Barcelo J (2006) Metals defend plants against biotic stress? Trends Plant Sci 11:288–295
Qureshi MI, Abdin MZ, Qadir S, Iqbal M (2007) Lead induced oxidative stress and metabolic alterations in Cassia angustifolia Vahl. Biol Plant 51:121–128
Babu TS, Akhtar TA, Lampi MA, Tripuranthakam S, Dixon DG, Greenberg BM (2003) Similar stress responses are elicited by copper and ultraviolet radiation Aquatic plant Lemna gibba: implication of reactive oxygen species as common signals. Plant Cell Physiol 44:1320–1329
Mishra S, Agarwal SB (2006) Interactive effects between supplemental ultraviolet-B radiation and heavy metals on the growth and biochemical characteristics of Spinacia oleracea L, Braz. J Plant Physiol 18:307–314
Otero S, Olivera EN, Abaigar JM, Tomas R, Crespo MA, Beaucourt N (2006) Effects of cadmium and enhanced UV radiation on the physiology and the concentration of UV-absorbing compounds of the aquatic liverwort Jungermannia exsertifolia subsp. Cordifolia. Photochem Photobiol Sci 5:760–769
Murphy A, Taiz L (1995) Comparison of metallothionein gene expression and nonprotein thiols in ten Arabidopsis ecotypes. Correlation with copper tolerance. Plant Physiol 109:945–954
Chongpraditnun P, Suzuki K, Kawaharada U, Nakajima K, Chino M (1991) Immunohistochemical localization of metallothionein in plant tissues. Water Air Soil Pollut 57–58:545–553
Hernandez MG, Murphy A, Taiz L (1998) Metallothioneins 1 and 2 have distinct but overlapping expression patterns in Arabidopsis. Plant Physiol 118:387–397
Mikio S, Yanai T, Hasegawa H, Akata N, Kudo SK, Matsumoto T, Noda Y, Otsu H, Sato F (1998) Concentration of metallothionein in mice livers after a small dose of irradiation. J Radiat Res 39:239–242
Ono S, Cai L, Cherian MG (1998) Effect of gamma radiation on levels of brain metallothionein and lipid peroxidation in transgenic mice. Radiat Res 150:52–57
Ablett E, Whiteman DC, Boyle GM, Green AC, Parsons PG (2003) Induction of MT in human skin by routine exposure to sunlight: evidence for a systemise response and enhanced induction at certain body sites. J Invest Dermatol 120:318–324
Williams LE, Mills RF (2005) P1B-ATPases—an ancient family of transition metal pumps with diverse functions in plants. Trends Plant Sci 10:491–502
Panda SK, Choudhury S (2005) Chromium stress in plants. Braz J Plant Physiol 17:95–102
Krouma A, Drevon JJ, Abdelly C (2006) Genotypic variation of N2-fixing common bean (Phaseolus vulgaris L.) in response to iron deficiency. J Plant Physiol 163:1094–1100
Bouazizi H, Jouili H, Ferjani EI (2007) Copper-induced oxidative stress in maize shoots (Zea mays L.): H2O2 accumulation and peroxidases modulation. Acta Biol Hung 58:209–218
Maksymiec W, Wojcik M, Krupa Z (2007) Variation in oxidative stress and photochemical activity in Arabidopsis thaliana leaves subjected to cadmium and excess copper in the presence or absence of jasmonate and ascorbate. Chemosphere 66:421–427
Winge DR, Nielson KB, Gray WR, Hamer DH (1985) Yeast metallothionein. Sequence and metal binding properties. J Biol Chem 260:14464–14470
Duncan KER, Ngu TT, Chan J, Salgado MT, Merrifield ME, Stillman MJ (2006) Protein folding metal-binding mechanisms and binding site structure in metallothioneins. Exp Biol Med 231:1488–1499
Olabanji SO, Olubunmi P, Ceccato D, Buoso MC, Poli MD, Moschini G (2005) Evaluation of Nigerian animal feeds by particle induced X-ray emission. Biol Trace Elem Res 107:181–197
Olabanji SO, Adesina SK, Ceccato D, Buoso MC, Moschini G (2007) PIXE analysis of some medicinal plants used in cleaning teeth in Southwestern Nigeria. Biol Trace Elem Res 116:171–184
Yurkow EJ, Makhijani PR (1998) Flow cytometric determination of metallothionein levels in human peripheral blood lymphocytes: utility in environmental exposure assessment. J Toxicol Environ Health 54:445–457
Lee HC, Lin TY (2005) Isolation of plant nuclei suitable for flow cytometry from recalcitrant tissue by use of a filtration column. Plant Mol Biol Report 23:53–58
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15:473–497
Chakraborty A, Selvaraj S, Sudarshan M, Dutta RK, Ghugre SS, Chintalapudi SN (2000) Modulatory role of vanadium on trace element profile in diethylnitrosamine-induced rat hepatocarcinogenesis. Nucl Instrum Methods B 170:156–162
Zhigang A, Cuijie L, Yuangang Z, Yejie D, Wachter A, Gromes R, Raush T (2006) Expression of BjMT2, a metallothionein 2 from Brassica juncea, increases copper and cadmium tolerance in Escherichia coli and Arabidopsis thaliana, but inhibits root elongation in Arabidopsis seedlings. J Exp Bot 57:3575–3582
Thornally PJ, Vasak M (1985) Possible role for metallothionein in protection against radiation-induced oxidative stress. Kinetics and mechanism of its reaction with superoxide and hydroxyl radicals. Biochim Biophys Acta 827:36–44
Matsubara J, Tajima Y, Karasawa M (1987) Metallothionein induction as a potent means of radiation protection in mice. Radiat Res 111:267–275
Koterov AN, Trebenok ZA, Pushkareva NB, Nikol’skii AV (1997) The diverse effects of the combined action of cadmium chloride and ionizing radiation on the content of metallothioneins in mouse bone marrow and liver. Radiat Biol Radioecol 37:196–201
Zhang H, Xu W, Guo J, He Z, Ma M (2005) Coordinated responses of phytochelations and metallothioneins to heavy metals in garlic seedlings. Plant Sci 169:1059–1065
Jin S, Cheng Y, Guan Q, Liu D, Takano T, Liu S (2006) A metallothionein-like protein of rice (rgMT) functions in E. coli and its gene expression is induced by abiotic stresses. Biotechnol Lett 28:1749–1753
Ramage CM, Williams RR (2003) Mineral uptake in tobacco leaf discs during different developmental stages of shoot organogenesis. Plant Cell Rep 21:1047–1053
Kintzios S, Drossopoulos JB, Lymperopoulos C (2001) Effect of vitamins and inorganic micronutrients on callus growth and somatic embryogenesis from leaves of chilli pepper. Plant Cell Tissue Organ Cult 67:55–62
Roy S, Begum Y, Chakraborty A, Sen Raychaudhuri S (2006) Radiation-induced phenotypic alterations in relation to isozymes and RAPD markers in Vigna radiata (L.) Wilczek. Int J Radiat Biol 82:823–832
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Saha, P., Mishra, D., Chakraborty, A. et al. In Vitro Radiation Induced Alterations in Heavy Metals and Metallothionein content in Plantago ovata Forsk. Biol Trace Elem Res 124, 251–261 (2008). https://doi.org/10.1007/s12011-008-8141-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12011-008-8141-8