Abstract
To address the high demand for Pueraria candollei var. mirifica (PM) used as the active ingredient in health products and its difficulty to cultivate in the field, the growth and production of deoxymiroestrol (DME) and isoflavonoid (ISF) phytoestrogens in PM cell suspensions were studied. In a 125-mL shake flask, the cell suspension produced DME [78.7 ± 8.79–116 ± 18.2 μg/g dry weight (DW)] and ISF (140 ± 6.83–548 ± 18.5 μg/g DW), which are the predominant ISF glycosides. While ISF aglycones accumulated in the PM cell suspension cultured in the airlift bioreactor. The DME content was increased to 976 ± 79.6 μg/g DW when the PM cell suspension was cultured in the 5-L scale bioreactor. The production of DME and ISF was enhanced by elicitors including methyl jasmonate (MJ), yeast extract (YE), and chitosan (CHI). MJ produced the highest induction of DME accumulation, while ISF accumulation was the highest with YE treatment. Analysis of catalase activity implied that the elicitors enhanced ROS production, which resulted in the enhancement of DME and ISF production and accumulation in PM cell suspension cultures. PM cell suspension culture is a promising source of beneficial PM phytoestrogens that exhibit bioactivity that may useful for the treatment of menopausal symptoms.
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Acknowledgments
The authors thank Dr. Chaiyo Chaichantipyuth at the Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand, for providing authentic DME and KWA.
Funding
This research was financially supported by the Faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand, and The Thailand Research Fund (IRN 61W0005).
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Udomsin, O., Yusakul, G., Kitisripanya, T. et al. The Deoxymiroestrol and Isoflavonoid Production and Their Elicitation of Cell Suspension Cultures of Pueraria candollei var. mirifica: from Shake Flask to Bioreactor. Appl Biochem Biotechnol 190, 57–72 (2020). https://doi.org/10.1007/s12010-019-03094-y
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DOI: https://doi.org/10.1007/s12010-019-03094-y