Skip to main content
SpringerLink
Log in

Immobilization of Xylanase on Poly (Ethylene Glycol) Methyl Ether 5000 and its Self-Extractive Bioconversion for the Production of Xylo-Oligosaccharides

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Endo-β-1,4-xylanase derived from Trichoderma reesei was covalently immobilized on poly (ethylene glycol) methyl ether 5000 (mPEG5000), and the resulting immobilized enzyme had a residual activity of 72.4 % with 82.9 % of PEGylated amino groups. Compared with the free enzyme, the immobilized xylanase was stable at pH values in the range of 4.0–6.0 and temperatures in the range of 50–65 °C. A self-extractive bioconversion system composed of immobilized xylanase, mPEG5000, and sodium citrate was used to produce xylo-oligosaccharides and provided a better distribution of the xylo-oligosaccharides than the free enzyme. Furthermore, the immobilized xylanase could be effectively recovered in situ following the hydrolysis reaction.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Vázquez, M. J., Alonso, J. L., Domínguez, H., & Parajó, J. C. (2000). Trends in Food Science & Technology, 11, 387–393.

    Article  Google Scholar 

  2. Dhiman, S. S., Jagtap, S. S., Jeya, M., Haw, J. R., Kang, Y. C., & Lee, J. K. (2012). Biotechnology Letters, 34, 1307–1313.

    Article  CAS  Google Scholar 

  3. Kapoor, M., & Kuhad, R. C. (2007). Applied Biochemistry and Biotechnology, 142, 125–138.

    Article  CAS  Google Scholar 

  4. Dumitriu, S., & Chornet, E. (1997). Biotechnology Progress, 13, 539–545.

    Article  CAS  Google Scholar 

  5. Sardar, M., Roy, I., & Gupta, M. N. (2000). Enzyme and Microbial Technology, 27, 672–679.

    Article  CAS  Google Scholar 

  6. Lin, Y. S., Tseng, M. J., & Lee, W. C. (2011). Process Biochemistry, 46, 2117–2121.

    Article  CAS  Google Scholar 

  7. Ai, Z., Jiang, Z., Li, L., Deng, W., Kusakabe, I., & Li, H. (2005). Process Biochemistry, 40, 2707–2714.

    Article  CAS  Google Scholar 

  8. Ouyang, J., Wang, X. M., Zhang, Q., Yan, M., & Xu, L. (2007). Chem. Indus. Forest. Prod., 27, 83–88.

    CAS  Google Scholar 

  9. Jeong, Y. I., Kim, D. G., Jang, M. K., & Nah, J. W. (2008). Carbohydrate Research, 343, 282–289.

    Article  CAS  Google Scholar 

  10. Li, X., Lian, Z., Dong, B., Xu, Y., Yong, Q., & Yu, S. (2011). Korean Journal of Chemical Engineering, 28, 1897–1901.

    Article  CAS  Google Scholar 

  11. Haynes, R., Osuga, D. T., & Feeney, R. E. (1967). Biochemistry, 6, 541–547.

    Article  CAS  Google Scholar 

  12. Veronese, F. M. (2009). PEGylated protein drugs: basic science and clinical applications. Basel, Switzerland: Birkhäuser Verlag.

    Book  Google Scholar 

  13. Törrönen, A., & Rouvinen, J. (1995). Biochemistry, 34, 847–856.

    Article  Google Scholar 

  14. Liu, S., Sun, L., Wang, J., Ma, G., Su, Z., & Hu, T. (2012). Process Biochemistry, 47, 1364–1370.

    Article  CAS  Google Scholar 

  15. Bora, U., Kannoujia, D. K., Kumar, S., Sharma, P., & Nahar, P. (2011). Process Biochemistry, 46, 1380–1383.

    Article  CAS  Google Scholar 

  16. Tischer, W., & Kasche, V. (1999). Trends in Biotechnology, 17, 326–335.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to acknowledge the financial support received from the Key Technology R&D Program of Jiangsu Province of China (grant no. BE2010732), the Natural Science Foundation of Jiangsu Province (grant no. BK20131426), the National Natural Science Foundation of China (grant no. 31000278), the Research Fund for the Doctoral Program of Higher Education of China (grant no. 20103204120009), the Excellent Youth Foundation of Jiangsu Province of China (grant no. BK2012038), and the Priority Academic Program Development of the Jiangsu Higher Education Institutions.

Author information

Authors and Affiliations

  1. Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, College of Chemical Engineering, Nanjing Forestry University, No. 159 Long Pan Road, Nanjing, 210037, Jiangsu Province, China

    Xin Li, Zongxing Shan, Xiangyang Song, Jia Ouyang, Yong Xu, Qiang Yong & Shiyuan Yu

Authors
  1. Xin Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zongxing Shan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiangyang Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jia Ouyang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yong Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Qiang Yong
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Shiyuan Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Qiang Yong.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, X., Shan, Z., Song, X. et al. Immobilization of Xylanase on Poly (Ethylene Glycol) Methyl Ether 5000 and its Self-Extractive Bioconversion for the Production of Xylo-Oligosaccharides. Appl Biochem Biotechnol 172, 2022–2029 (2014). https://doi.org/10.1007/s12010-013-0666-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-013-0666-4

Keywords

Search

Navigation