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Thermostability of Cromobacterium viscosum lipase in AOT/isooctane reverse micelle

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Abstract

The thermostability of Cromobacterium viscosum lip ase (EC 3.1.1.3) entrapped in AOT (sodium bis-[2-ethylhexyl] sulfosuccinate) reverse micelles was in creased by the addition of short-chain polyethylene glycol (PEG 400). Two different approaches were considered: (1) the determination of half-life time and (2) the mechanistic analysis of deactivation kinetics. The half-life of lipase entrapped in AOT/isooctane reverse micelles with PEG 400 at 60°C was 28h, ninefold higher than that in reverse micelles without PEG 400. The lip ase entrapped in both reverse micellar systems followed a series-type deactivation mechanism involving two first-order steps. The deactivation constant for the first step at 60°C in PEG containing reverse micelles was 0.055 h11, 11-fold lower than that in reverse micelles without PEG, whereas it remained almost constant for the second step. The inactivation energy of the lip ase entrapped in reverse micelles with and without PEG 400 was 88.12 and 21.97 kJ/mol, respectively.

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Authors and Affiliations

  1. Institute of Chemical and Engineering Sciences, 1 Pesek Road, Jurong Island, 627833, Singapore

    M. M. R. Talukder & J. C. Wu

  2. Department of Chemistry and Chemical Engineering, Faculty of Engineering, Kanazawa University, 2-40-20 Kodatsuno, 920-8667, Kanazawa, Japan

    M. M. Zaman, Y. Hayashi & T. Kawanishi

Authors
  1. M. M. R. Talukder
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  2. M. M. Zaman
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  3. Y. Hayashi
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  4. J. C. Wu
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  5. T. Kawanishi
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Correspondence to M. M. R. Talukder.

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Talukder, M.M.R., Zaman, M.M., Hayashi, Y. et al. Thermostability of Cromobacterium viscosum lipase in AOT/isooctane reverse micelle. Appl Biochem Biotechnol 141, 77–83 (2007). https://doi.org/10.1007/s12010-007-9211-7

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  • DOI: https://doi.org/10.1007/s12010-007-9211-7

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