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Effect of cyclic and acyclic surfactants on the activity of Candida rugosa lipase

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Abstract

The active site of Candida rugosa lipase (CRL) is mainly hydrophilic on its external face and hydrophobic on the internal side, and calix[n]arene-based surfactants form complexes with protein residues or with strong hydrogen bonds to open up the lid. Therefore, the activity of lipase persists for a long time. In this work, a series of cyclic and acyclic anionic surfactants (sodium dodecyl sulfate (SDS), p-sulfonatocalix[4]arene, and p-sulfonatocalix[8]arene) and zwitterionic surfactants (l-proline and l-proline derivative of calix[4]arene) were used to examine the relationship between the surfactants’ molecular structures and their effects on the hydrolytic activity of CRL. We explored the effects of different surfactant concentrations, ring effects, and mixing times on CRL activity and several kinetic parameters. The results demonstrated that cyclic compounds were more effective than linear structures for increasing CRL activity and the highest enzyme activity was obtained by the addition of the calix[4]-l-proline derivative. This zwitterionic compound (calix[4]-l-proline derivative) maintains the active center of enzyme and conformation by enabling electrostatic interactions and hydrogen bonding with both the acidic and basic amino acid groups in the structure of the enzyme. The results indicated that, compared with the other surfactants, activating CRL with calix[4]-l-proline resulted in hyperactivation at all concentrations (a relative increase of 230%).

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Acknowledgements

The authors thank the Scientific Research Projects Foundation of Selcuk University (SUBAP Grant number 19201030) for financial support of this work produced from a part of F. Eski’s Master's thesis.

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  1. Department of Biochemistry, Selcuk University, 42075, Konya, Turkey

    Elif Ozyilmaz

  2. Department of Chemistry, Selcuk University, 42075, Konya, Turkey

    Filiz Eski

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449_2020_2397_MOESM1_ESM.docx

Supplementary file1Synthesis and NMR studies of p-sulfonatocalix[4]arene, p-sulfonatocalix[8]arene and calix [4]arene l-proline derivative. Enzyme activity results at different concentrations of non-cyclic anionic SDS, ring-shaped p-sulfonatocalix[4]arene and p-sulfonatocalix[8]arene, zwitterionic l-proline and calix[4]arene l-proline derivative (DOCX 2246 kb)

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Ozyilmaz, E., Eski, F. Effect of cyclic and acyclic surfactants on the activity of Candida rugosa lipase. Bioprocess Biosyst Eng 43, 2085–2093 (2020). https://doi.org/10.1007/s00449-020-02397-3

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