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Biosynthesis of polyhydroxyalkanoates from sugarcane molasses by recombinant Ralstonia eutropha strains

  • Biotechnology
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Abstract

Sugarcane molasses was examined for the production of poly(3-hydroxybutyrate) [P(3HB)] and poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)] in recombinant Ralstonia eutropha strains expressing Mannheimia succiniciproducens sacC gene encoding β-fructofuranosidase, which can hydrolyze sucrose into glucose and fructose in the culture medium. When crude sugarcane molasses was added to the culture medium to support 20 g/L of sucrose in flask cultivation, the growth of R. eutropha NCIMB11599 expressing the sacC gene was significantly inhibited, which resulted in OD600 of 1.2 with P(3HB) content of 0.1wt%. The inhibition of cell growth due to the usage of the crude sugarcane molasses was relieved by pretreatment of sugarcane molasses with activated charcoal. Sugarcane molasses pretreated with activated charcoal could support the growth of R. eutropha NCIMB11599 expressing the sacC gene to OD600 of 87.2 with P(3HB) content of 82.5 wt% in batch fermentation when it was added to culture medium to support 20 g/L of sucrose. Also, R. eutropha 437–540 expressing Escherichia coli ldhA gene encoding lactate dehydrogenase along with the sacC gene produced P(3HB-co-6.2 mol%LA) with 29.1 wt% polymer content from sugarcane molasses in batch fermentation.

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References

  1. B. Pang, L. E. Valencia, J. Wang, Y. Wan, R. Lal, A. Zargar and J. D. Keasling, Biotechnol. Bioprocess Eng., 24(3), 1 (2019).

    Article  Google Scholar 

  2. M. N. Rhie, H. T. Kim, S. Y. Jo, L. L. Chu, K. A. Baritugo, M. G. Baylon, J. Lee, J. G. Na, L. H. Kim, T. W. Kim, C. Park, S. H. Hong, J. C. Joo and S. J. Park, Biotechnol. Bioprocess Eng, 24(1), 48 (2019).

    Article  CAS  Google Scholar 

  3. R. Geyer, J. R. Jambeck and K. L. Law, Sci. Adv., 3, e1700782 (2017).

    Article  PubMed  PubMed Central  Google Scholar 

  4. Y. J. Sohn, H. T. Kim, K. A. Baritugo, S. Y. Jo, H. M. Song, S. Y. Park, S. K. Park, J. W. Pyo, H. G. Cha, H. Kim, J. G. Na, C. Park, J. I. Choi, J. C. Joo and S. J. Park, Biotechnol. J., 15(6), 1900489 (2020).

    Article  CAS  Google Scholar 

  5. S. Y. Choi, M. N. Rhie, H. T. Kim, J. C. Joo, I. J. Cho, J. N. Son, S. Y. Jo, Y. J. Sohn, K. A. Baritugo, J. W. Pyo, Y. J. Lee, S. Y. Lee and S. J. Park, Metab. Eng., 58, 47 (2020).

    Article  CAS  PubMed  Google Scholar 

  6. X. Wang, X. Zhang and H. Lu, Korean J. Chem. Eng., 37(2), 249 (2020).

    Article  CAS  Google Scholar 

  7. S. J. Park, Y. A. Jang, H. Lee, A. R. Park, J. E. Yang, J. Shin, Y. H. Oh, B. K. Song, J. Jegal, S. H. Lee and S. Y. Lee, Metab. Eng., 20, 20 (2013).

    Article  CAS  PubMed  Google Scholar 

  8. T. U. Khang, M. J. Kim, J. I. Yoo, Y. J. Sohn, S. G. Jeon, S. J. Park and J. G. Na, Int. J. Biol. Macromol., 174, 449 (2021).

    Article  CAS  PubMed  Google Scholar 

  9. Y. Zheng, J. C. Chen, Y. M. Ma and G. Q. Chen, Metab. Eng., 58, 82 (2020).

    Article  CAS  PubMed  Google Scholar 

  10. G. Q. Chen, X. Y. Chen, F.Q. Wu and J. C. Chen, Adv. Ind. Eng. Polym. Res., 3(1), 1 (2020).

    Google Scholar 

  11. J. I. Choi and S. Y. Lee, Appl. Environ. Microbiol., 65(10), 4363 (1999).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. W. Lu, M. A. Alam, W. Luo and E. Asmatulu, Bioresour. Technol., 271, 59 (2019).

    Article  CAS  PubMed  Google Scholar 

  13. P. K. Dikshit, H. B. Jun and B. S. Kim, Korean J. Chem. Eng., 37(3), 387 (2020).

    Article  CAS  Google Scholar 

  14. S. K. Bhatia, J. J. Yoon, H. J. Kim, J. W. Hong, Y. G. Hong, H. S. Song, Y.M. Moon, J.M. Jeon, Y.G. Kim and Y. H. Yang, Bioresour. Technol., 257, 92 (2018).

    Article  CAS  PubMed  Google Scholar 

  15. Y. H. Oh, S. H. Lee, Y. A. Jang, J. W. Choi, K. S. Hong, J. H. Yu, J. Shin, B. K. Song, S. G. Mastan, Y. David, M. G. Baylon, S. Y. Lee and S. J. Park, Bioresour. Technol., 181, 283 (2015).

    Article  CAS  PubMed  Google Scholar 

  16. M. G. E. Albuquerque, M. Eiroa, C. Torres, B. R. Nunes and M. A. M. Reis, J. Biotechnol., 130(4), 411 (2007).

    Article  CAS  PubMed  Google Scholar 

  17. S. Bengtsson, A. R. Pisco, P. Johansson, P. C. Lemos and M. A. M. Reis, J. Biotechnol., 147(3), 172 (2010).

    Article  CAS  PubMed  Google Scholar 

  18. E. Akaraonye, C. Moreno, J. C. Knowles, T. Keshavarz and I. Roy, Biotechnol. J., 7(2), 293 (2012).

    Article  CAS  PubMed  Google Scholar 

  19. S. Chan, S. Kanchanatawee and K. Jantama, Bioresour. Technol., 103(1), 329 (2012).

    Article  CAS  PubMed  Google Scholar 

  20. M. Y. Jung, B. S. Park, J. Lee and M. K. Oh, Bioresour. Technol., 139, 21 (2013).

    Article  CAS  PubMed  Google Scholar 

  21. Z. Nofemele, P. Shukla, A. Trussler, K. Permaul and S. Singh, J. Brewing Distilling., 3(2), 29 (2012).

    CAS  Google Scholar 

  22. M. E. Ortiz, M. J. Fornaguera, R. R. Raya and F. Mozzi, Appl. Microbiol. Biotechnol., 95(4), 991 (2012).

    Article  CAS  PubMed  Google Scholar 

  23. M. Samavi and S. Rakshit, Biotechnol. Bioprocess Eng., 25(2), 327 (2020).

    Article  CAS  Google Scholar 

  24. R. Sindhu, E. Gnansounou, P. Binod and A. Pandey, Renew. Energy, 98, 203 (2016).

    Article  CAS  Google Scholar 

  25. S. C. Bhatia, Advanced renewable energy systems, WPI Publishing, New Delhi (2014).

    Google Scholar 

  26. M. K. Gouda, A. E. Swellam and S. H. Omar, Microbiol. Res., 156(3), 201 (2001).

    Article  CAS  PubMed  Google Scholar 

  27. M. A. Renouf, M. K. Wegener and L. K. Nielsen, Biomass Bioenerg., 32(12), 1144 (2008).

    Article  CAS  Google Scholar 

  28. J. W. Lee, S. Choi, J. H. Kim and S. Y. Lee, Appl. Environ. Microbiol., 76(5), 1699 (2010).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. J. W. Lee, S. Choi, J. H. Park, C. E. Vickers, L. K. Nielsen and S. Y. Lee, Appl. Microbiol. Biotechnol., 88(4), 905 (2010).

    Article  CAS  PubMed  Google Scholar 

  30. Y. Y. Zhang, Y. F. Bu and J. Z. Liu, Folia Microbiol., 60(5), 393 (2015).

    Article  CAS  Google Scholar 

  31. O. Martinez, A. Sanchez, X. Font and R. Barrena, Bioresour. Technol., 263, 136 (2018).

    Article  CAS  PubMed  Google Scholar 

  32. Y. P. Liu, P. Zheng, Z. H. Sun, Y. Ni, J. J. Dong and L. L. Zhu, Bioresour. Technol., 99(6), 1736 (2008).

    Article  CAS  PubMed  Google Scholar 

  33. S. Xu, N. Hao, L. Xu, Z. Liu, M. Yan, Y. Li and P. Ouyang, Biochem. Eng. J., 99, 177 (2015).

    Article  CAS  Google Scholar 

  34. C. Andreeßen and A. Steinbüchel, Appl. Microbiol. Biotechnol., 103(1), 143 (2019).

    Article  PubMed  Google Scholar 

  35. H. Seo and K. J. Kim, Biotechnol. Bioprocess Eng., 24(1), 155 (2019).

    Article  CAS  Google Scholar 

  36. Y. Zheng, S. Tianyuan and Q. Qingsheng, Biotechnol. Bioprocess Eng., 24(4), 579 (2019).

    Article  CAS  Google Scholar 

  37. S. J. Park, Y. A. Jang, W. Noh, Y. H. Oh, H. Lee, Y. David, M. G. Baylon, J. Shin, J. E. Yang, S. Y. Choi, S. H. Lee and S. Y. Lee, Biotechnol. Bioeng., 112(3), 638 (2015).

    Article  CAS  PubMed  Google Scholar 

  38. Y. J. Sohn, H. T. Kim, K. A. Baritugo, H. M. Song, M. H. Ryu, K. H. Kang, S. Y. Jo, H. Y. Kim, Y. J. Kim, J. I. Choi, S. K. Park, J. C. Joo and S. J. Park, Int. J. Biol. Macromol., 149, 593 (2020).

    Article  CAS  PubMed  Google Scholar 

  39. G. Brauegg, B. Sonnleitner and R. Lafferty, Eur. J. Appl. Microbiol. Biotechnol., 6(1), 29 (1978).

    Article  Google Scholar 

  40. C. Gong, C. Chen and L. Chen, Appl. Biochem. Biotechnol., 39(1), 83 (1993).

    Article  Google Scholar 

  41. S. Baruah, M. N. Khan and J. Dutta, Environ. Chem. Lett., 14(1), 1 (2016).

    Article  CAS  Google Scholar 

  42. R. Gupta, S. Gautam, R. Shukla and R. C. Kuhad, J. Environ. Chem. Eng., 5(5), 4573 (2017).

    Article  CAS  Google Scholar 

  43. C. Sarawan, T. N. Suinyuy, Y. Sewsynker-Sukai and E. G. Kana, Bioresour. Technol., 286, 121403 (2019).

    Article  CAS  PubMed  Google Scholar 

  44. M. C. Rubio and M. C. Maldonado, Curr. Microbiol., 31(2), 80 (1995).

    Article  CAS  Google Scholar 

  45. C. Sirisatesuwon, B. Ninchan and K. Sriroth, Sugar Tech, 22(2), 274 (2020).

    Article  CAS  Google Scholar 

  46. M. A. Ehrmann, M. Korakli and R. F. Vogel, Curr. Microbiol., 46(6), 0391 (2003).

    Article  CAS  Google Scholar 

  47. A. Tosun and M. Ergun, J. Chem. Technol. Biotechnol., 82(1), 11 (2007).

    Article  CAS  Google Scholar 

  48. S. Chotineeranat, R. Wansuksri, K. Piyachomkwan, P. Chatakanonda, P. Weerathaworn and K. Sriroth, Sugar Tech, 12(2), 120 (2010).

    Article  CAS  Google Scholar 

  49. E. Volodina, M. Raberg and A. Steinbüchel, Crit. Rev. Biotechnol., 36(6), 978 (2016).

    Article  CAS  PubMed  Google Scholar 

  50. M. S. Baei, G. Najafpour, H. Younesi, F. Tabandeh and H. Eisazadeh, World Appl. Sci. J., 7(2), 157 (2009).

    Google Scholar 

  51. M. Ul-Islam, M. W. Ullah, S. Khan and J. K. Park, Korean J. Chem. Eng., 37, 925 (2020).

    Article  CAS  Google Scholar 

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Acknowledgements

We thank Dr. II-Kwon Kim (DAESANG Corp., Republic of Korea) for providing sugarcane molasses. This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science and ICT (MSIT) through the National Research Foundation (NRF) of Korea (NRF-2015M1A2A2035810), the Basic Science Research Program (NRF-2020R1F1A1070249) and the NRF grant funded by the MSIT (NRF-2020R1A5A1019631).

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Authors and Affiliations

  1. Department of Chemical Engineering and Materials Science, Graduate Program in System Health Science and Engineering, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul, 03760, Korea

    Seo Young Jo, Yu Jung Sohn, Se Young Park, Jina Son, Jee In Yoo, Kei-Anne Baritugo, Yokimiko David, Mi Na Rhie & Si Jae Park

  2. Bio-based Chemistry Center, Advanced Convergent Chemistry Division, Korea Research Institute of Chemical Technology, Daejeon, 34114, Korea

    Kyoung Hee Kang

  3. Bio-based Chemistry Research Center, Advanced Convergent Chemistry Division, Korea Research Institute of Chemical Technology (KRICT), Ulsan, 44412, Korea

    Hoyong Kim

  4. Department of Biotechnology and Engineering, Interdisciplinary Program of Bioenergy and Biomaterials, Chonnam National University, Gwangju, 61186, Korea

    Jong-il Choi

  5. Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, 34134, Korea

    Hee Taek Kim

  6. Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do, 14662, Korea

    Jeong Chan Joo

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  1. Seo Young Jo
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Correspondence to Hee Taek Kim, Jeong Chan Joo or Si Jae Park.

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Jo, S.Y., Sohn, Y.J., Park, S.Y. et al. Biosynthesis of polyhydroxyalkanoates from sugarcane molasses by recombinant Ralstonia eutropha strains. Korean J. Chem. Eng. 38, 1452–1459 (2021). https://doi.org/10.1007/s11814-021-0783-7

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  • DOI: https://doi.org/10.1007/s11814-021-0783-7

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