Abstract
The operational optimization of zeaxanthin production by Paracoccus zeaxanthinifaciens ATCC 21588 in a bubble column reactor was performed by coupling genetic algorithm (GA) to an artificial neural network (ANN) model developed using experimental one-variable-at-a-time (OVAT) results. The effects of varying air flow rate (2-5 vvm) and inoculum size (4 and 8%) for different incubation time (30-80 h) were evaluated. Volumetric power input (P/V L ) and energy input (E) to the bubble column were then correlated with the ANN-GA optimized conditions. A maximum zeaxanthin production of 13.76±0.14 mg/L was observed at 4 vvm using an inoculum size of 4% (v/v) after 60 h of incubation in OVAT experiments with corresponding P/V L value of 231.57 W/m3 reflecting an energy consumption of 50.02 kJ during the fermentation period. The ANN based GA optimization predicted a maximum zeaxanthin production of 14.79 mg/L at 3.507 vvm, 4% inoculum size and 55.83 h against the experimental production of 15.09±0.51 mg/L corresponding to a P/V L value of 202.03 W/m3 reflecting to a significantly reduced energy input (40.01 kJ). The proposed OVAT based ANN-GA optimization approach can be used to simulate similar studies involving microbial fermentation in bioreactors.
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J. D. Ribaya-Mercado and J. B. Blumberg, J. Am. Coll. Nutr., 23, 567S (2004).
E. Loane, J. M. Nolan, O. O’Donovan, P. Bhosale, P. S. Bernstein and S. Beatty, Surv. Ophthalmol., 53, 68 (2008).
R. L. Roberts, J. Green and B. Lewis, Clin. Dermatol., 27, 195 (2009).
S. M. Moeller, P. F. Jacques and J. B. Blumberg, J. Am. Coll. Nutr., 19, 522S (2000).
P. Thawornwiriyanun, S. Tanasupawat, C. Dechsakulwatana, S. Techkarnjanaruk and W. Suntornsuk, Appl. Biochem. Biotechnol., 167, 2357 (2012).
M. Y. Victor and S. K. Bhatia, Biotechnol. Lett., 34, 1405 (2012).
Y. T. Cheng and C. F. Yang, J. Taiwan Inst. Chem. Eng., 61, 270 (2016).
A. Berry, D. Janssens, M. Hümbelin, J. P. Jore, B. Hoste, I. Cleenwerck, M. Vancanneyt, W. Bretzel, A. F. Mayer, R. Lopez-Ulibarri and B. Shanmugam, Int. J. Syst. Evol. Microbiol., 53, 231 (2003).
A. J. Schocher and O. Wiss, US Patent, 3,891,504 (1975).
P.M. Doran, Bioprocess engineering principles, Academic Press, London (1995).
K. Nanou, T. Roukas and E. Papadakis, Biochem. Eng. J., 54, 172 (2011).
K. Nanou, T. Roukas and E. Papadakis, Biochem. Eng. J., 67, 203 (2012).
P. F. Stanbury, A. Whitaker and S. J. Hall, Principles of fermentation technology, Elsevier (2013).
L.M. Hao, L.Z. Xing, J.C. Zhang, J.X. Sun, S.R. Jia, C.S. Qiao and T. Wu, Appl. Biochem. Biotechnol., 160, 621 (2010).
D. Dursun and A.C. Dalgiç, Biocatal. Agric. Biotechnol., 7, 1 (2016).
E.B. Eryilmaz, D. Dursun and A.C. Dalgiç, Biocatal. Agric. Biotechnol., 7, 224 (2016).
K.R. Pandey, C. Joshi and B.V. Vakil, SpringerPlus, 5, 1654 (2016).
C. H. Kuo, T. A. Liu, J. H. Chen, C. M. J. Chang and C. J. Shieh, Biocatal. Agric. Biotechnol., 3, 1 (2014).
A.A. Babaei, A. Khataee, E. Ahmadpour, M. Sheydaei, B. Kakavandi and Z. Alaee, Korean J. Chem. Eng., 33, 1352 (2016).
D. Singh and G. Kaur, Bioproc. Biosyst. Eng., 37, 1599 (2014).
M. Zafar, S. Kumar and A. K. Dhiman, Biocatal. Agric. Biotechnol., 1, 70 (2012).
A.A. Prabhu, B. Mandal and V.V. Dasu, Korean J. Chem. Eng., 34, 1109 (2017).
P. Kundu and I. M. Mishra, Desalination Water Treat., 57, 19713 (2016).
S.M. Huang, C. H. Kuo, C. A. Chen, Y. C. Liu and C. J. Shieh, Ultrason. Sonochem., 36, 112 (2017).
Z. Ilbay, S. Sahin and K. Büyükkabasakal, Korean J. Chem. Eng., 31, 1661 (2014).
P. Kundu, V. Paul, V. Kumar and I. M. Mishra, Chem. Eng. Res. Des., 104, 773 (2015).
P. Kundu, V. Paul, V. Kumar and I. M. Mishra, Petrol. Sci. Technol., 34, 350 (2016).
E.M. E.M. Shokir, E. S. Al-Homadhi, O. Al-Mahdy and A. A.H. El-Midany, Korean J. Chem. Eng., 31, 1496 (2014).
P. Davoodi, S. M. Ghoreishi and A. Hedayati, Korean J. Chem. Eng., 34, 854 (2017).
M.G. Sajilata, M.V. Bule, P. Chavan, R. S. Singhal and M.Y. Kamat, Sep. Purif. Technol., 71, 173 (2010).
C. Joshi and R. S. Singhal, Biocatal. Agric. Biotechnol., 8, 228 (2016).
D. S. Savic, M. L. Lazic, V.B. Veljkovic and M. M. Vrvic, Chem. Ind. Chem. Eng., 11, 59 (2005).
K. Hirasawa and A. Tsubokura, U.S. Patent, 8,853,460 (2014).
J. Hirschberg and M. Harker, U.S. Patent, 5,935,808 (1999).
S. Rosa-Putra, A. Hemmerlin, J. Epperson, T. J. Bach, L. H. Guerra and M. Rohmer, FEMS Microbiol. Lett., 204, 347 (2001).
V. Ferreira, M.D.O. Faber, S.D. S. Mesquita and N. Pereira Jr., Electron. J. Biotechnol., 13, 5 (2010).
D. S. Clark and H.W. Blanch, Biochemical Engineering, CRC Press (1997).
P. Bhosale, A. J. Larson and P.S. Bernstein, J. Appl. Microbiol., 96, 623 (2004).
R. Verwaal, Y. Jiang, J. Wang, J. M. Daran, G. Sandmann, J. A. van den Berg and A. J. van Ooyen, Yeast, 27, 983 (2010).
R. Doshi, T. Nguyen and G. Chang, PNAS, 110, 7642 (2013).
S. Prabhu, P.D. Rekha, C. C. Young, A. Hameed, S.Y. Lin and A. B. Arun, Appl. Biochem. Biotechnol., 171, 817 (2013).
C. Sandhya, A. Sumantha, G. Szakacs and A. Pandey, Process Biochem., 40, 2689 (2005).
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Joshi, C., Singhal, R.S. Zeaxanthin production by Paracoccus zeaxanthinifaciens ATCC 21588 in a lab-scale bubble column reactor: Artificial intelligence modelling for determination of optimal operational parameters and energy requirements. Korean J. Chem. Eng. 35, 195–203 (2018). https://doi.org/10.1007/s11814-017-0253-4
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DOI: https://doi.org/10.1007/s11814-017-0253-4