Abstract
Climate changes have a direct impact on agricultural lands through their impact on the rate of water levels in the oceans and seas, which leads to a decrease in the amount of water used in agriculture, and therefore the use of alternative sources of irrigation such as wastewater and overcoming its harmful effect on plants was one of the solutions to face this problem. In the present study, the impacts of the synthesized selenium nanoparticles (Se NPs) alone or in combination with glycine betaine and proline treatments on the growth, physiological, and yield attributes of wastewater irrigated carrot plants are investigated. Furthermore, to evaluate heavy metals uptake and accumulation in edible plant parts. The usage of wastewater to carrot plants significantly increased free proline contents, total phenols, superoxide dismutase, catalase, peroxidase, polyphenol oxidase, Malondialdehyde (MDA), and hydrogen peroxide (H2O2) throughout the two growth stages. While total soluble carbohydrate and soluble protein content in carrot shoots and roots were significantly reduced. Moreover, the concentrations of nickel (Ni), cadmium (Cd), lead (Pb), and cobalt (Co) in carrot plants were considerably higher than the recommended limits set by international organizations. Application of selenium nanoparticles alone or in combination with glycine betaine and proline reduced the contents of Ni, Cd, Pb, and Co; free proline; total phenols; superoxide dismutase; catalase; peroxidase; polyphenol oxidase; Malondialdehyde (MDA) and Hydrogen peroxide (H2O2) in carrot plants. However, morphological aspects, photosynthetic pigments, soluble carbohydrates, soluble protein, total phenol, and β-Carotene were enhanced in response to Se NPs application. As an outcome, this research revealed that Se NPs combined with glycine betaine and proline can be used as a strategy to minimize heavy metal stress caused by wastewater irrigation in carrot plants, consequently enhancing crop productivity and growth.
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Introduction
One of the main issues for farmers is a lack of water, which tempts them to use wastewater for crop irrigation (Al-Zou’by et al. 2017). Different plant species have developed different mechanisms to cope with wastewater effects (Abu-Elela et al. 2021). The most common chemical contaminants in wastewater appear to be heavy metal cations, hydrocarbons, pesticides, nitrogenous compounds, pharmaceutical residues, detergents, and phosphorus (Agoro et al. 2020). Heavy metals (HMs) accumulation has received a lot of attention in recent years and becoming a more serious issue as a consequence of rapid industrialization and urbanization, the prevalent use of herbicides, pesticides, fertilizers, irrational quarrying and waste management practices (Li et al. 2021). HMs stress has an impact on plant growth as well as, indirectly, public health via the food system (Gupta et al. 2019). Also, Pandey et al. (2019) reported that nickel ion inhibited germination rate and early seedling growth of maize and rice; it suppressed germination and early germination percentage of maize and rice in regards to germination rate of seeds, length of roots and roots; the fresh and dry weight of seedlings. Moreover, Yaqvob et al. (2011) indicated that Fe, Pb, and Cu were found to reduce all tomato plant growth parameters.
Nanoparticles (NPs) have seen an increase in their use in industry, medicine, agriculture, and cosmetics in recent times (Adeel et al. 2019). NPs have several benefits over traditional materials, including high surface activity, more surface reaction sites, superior catalytic efficiency, and exceptional optical and magnetic characteristics (Wang et al. 2019). NPs (such as CeO2, TiO2, and Mn3O4 NPs) could indeed boost antioxidant enzyme activity, which also reduces the accumulation of reactive oxygen species (ROS) in plants, ameliorating plant stress and enhancing quality and yield (Usman et al. 2020). The progress of nanoscience opens up a new avenue for the advancement of soil remediation (Liu et al. 2021). In rice plants, foliar application of selenium and silicon NPs reduced Cd and Pb stress (Hussain et al. 2020; Soliman et al. 2022). In terms of reducing Cd toxicity in maize, foliar spraying of titanium dioxide (TiO2) NPs outperforms soil addition (Lian et al. 2020). The use of glycine betaine (GB) and proline on plants under heavy metal stress improves growth, photosynthetic activity, oxidative stress, nutrient uptake, and reduces excessive heavy metal uptake and oxidative stress. Furthermore, GB regulates glutathione reductase (GR), ascorbic acid (AsA), and glutathione (GSH) levels in plants under HM stress. An excessive amount of GB via a genetic engineering approach can successfully improve stress tolerance, which is considered as a significant characteristic that requires further investigation (Ali et al. 2020). Proline serves as an organic osmo protectant that accumulates in plants at quite large levels when they are exposed to abiotic stressors (Dar et al. 2016). It also has a variety of activities in plants, including stress tolerance, signaling, radical scavenging, and protein stabilization, as well as acting as a stress receptor (Hayat et al. 2013).
The Apiaceae family’s most important crop is the carrot (Daucus carota L.). It is a root vegetable that is found all over the world. Carrots were cultivated in Europe prior to the tenth century, according to written records. Orange carrots, which are more popular today, were developed in Central Europe during the 15th and 16th centuries. With the recognition of their high provitamin A content, orange carrots have seen a rapid rise in popularity (Iorizzo et al. 2016). Carotenoids and anthocyanins are the most abundant antioxidant pigments in carrots. Carrot cultivar variations are determined by the pigments present. Carotenoids are phytochemicals that are yellow, orange, or red in colour and are observed in most yellow and orange cultivars. The common orange carrot is high in α- and β-carotene and an excellent source of provitamin A (Dias 2012). Different researchers as well carefully investigated in Egypt the impact of wastewater irrigation on various plant species. In this regard, Elgharably and Mohamed (2016) and Merwad (2019) investigated the accumulation of heavy metals in wheat, bean, and onion plants that have been irrigated with wastewater and noticed that, in comparison to plants that were irrigated with ground water, wastewater-irrigated wheat plants had greater concentrations of Zn, Mn, Pb, Ni, and Cd in their edible parts (grains). The present study was conducted to assess whether the application of selenium NPs individually or combined with glycine betaine and proline could ameliorate the harmful effects of wastewater on carrot plants. We combined nano selenium with glycine betaine and proline in this study because they are valuable amino acids with osmoprotectant properties, and their levels vary greatly between different plants. Investigating the physiological, biochemical and yield parameters in response to our treatments. In addition to estimating heavy metals uptake and accumulation in carrot root (edible part).
Materials and methods
Chemicals and reagents
Hi-Media and Difco supplied the media ingredients and components. Chemical compounds including selenoius acid as well as other reagents (used in the following methods) were purchased from (Sigma-Aldrich, Saint Louis, Missouri, United States) at quantitative standard grade.
Seeds and water sampling
The seeds of carrot (Daucus carota) were obtained from Agricultural Research Centre, Ministry of Agriculture, Giza, Egypt. Water samples were collected from each irrigation source (fresh tap water and wastewater effluent). Wastewater samples were collected from El-Rahawy drain (30°12`13.3” N latitude and 31°03′54.3” E longitude), Giza, Egypt, which receives all sewage from El-Giza governorate in addition to agricultural and domestic wastes of El-Rahway village without treatment. The wastewater samples were collected in plastic bottles and used in irrigation.
Biosynthesis of Se NPs using glutathione (GSH)
Under stirring, selenious acid (0.04 mM) was mixed with glutathione (GSH) as a product of Saccharomyces cerevisiae (0.2 mM) and 200 mg bovine albumin solution in 100 mL deionized water. To begin the reaction, the pH of the mixture was adjusted to 7.2 with 1.0 M sodium hydroxide. Under sonication, the reaction lasted one hour and produced red elemental Se and oxidized glutathione (GSSG). To separate GSSG from Se NPs, the red solution was dialyzed against double distilled water for 96 h, with the water changing every 24 h.
Characterization of the synthesized biogenic Se NPs
UV-Vis. spectrophotometer (JASCO V-560. UV-Vis. spectrophotometer) represented the optical behavior of the fabricated Se NPs (El-Sayyad et al. 2020), and were compared to a negative control, which consisted of reaction mixture without selenium salt. The XRD-6000 lists, Shimadzu apparatus, SSI, Japan, were used to determine the crystallinity, crystallite size, and lattice of the produced Se NPs. The diffracted X- rays’ intensity was calculated as diffracted angle 2θ (El-Batal et al. 2020). The most prevailing Se NPs particle size distribution, hydrodynamic radius, and polydispersity index (PDI) were determined by Dynamic Light Scattering (Zaki et al. 2022) (DLS-PSS-NICOMP 380-USA). Further, the mean particle size, the microstructure, and the estimated shape of the biosynthesized Se NPs were assessed by High-Resolution Transmission Electron Microscope (HRTEM, JEM2100, Jeol, Japan) (Ashour et al. 2018). SEM, ZEISS, EVO-MA10, Germany, was used to study the grain size and surface morphology of Se NPs. Besides, the EDX study (BRUKER, Nano GmbH, D-12,489, 410-M, Germany) was operated to evaluate the Se elemental configuration and the investigated purity (Maksoud et al. 2020).
Methods of planting, treatments, and collection of plant samples
A field experiment was carried out during winter season of 2020–2021 under the natural environmental conditions at Botanical Garden, Botany and Microbiology Departement, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt. The carrot seeds were planted in pots (30 cm diameter) filled with 6.0 kg of clay soil. The pots were divided into 6 groups representing the following treatments: I- Fresh water (Control); II- Wastewater; III- Fresh water + Se NPs (10 ppm); IV- Fresh water + Composite of (Se NPs (10 ppm) + proline (50 ppm) and glycine betaine (50 ppm)); V- Wastewater + Se NPs (10 ppm); VI- Wastewater + composite of (Se NPs (10 ppm) + proline (50 ppm) and glycine betaine (50 ppm)). Plants in each group were treated twice (as a foliar spray) with the aforementioned treatments at 30 and 45 days after sowing. Irrigation was provided to the developed plants as needed until complete germination. The plant samples were collected for analysis at 37 (Stage I) and 52 (Stage II) days after planting. At the end of the growing season (120 days), yields from the various treatments and the control were analyzed.
Analyses of wastewater
Heavy metals contents in fresh tap water and wastewater were estimated according (Association 1995). Salinity (EC), total dissolved solids (TDs), and pH were measured (Table 1), at the atomic spectroscopy laboratory, arid land agricultural research and services center, faculty of agriculture, Ain Shams University, Cairo, Egypt.
Plant analyses
Determination of metabolic contents
Contents of total soluble proteins were assayed according to the methods of (Lowry 1951). Contents of free proline were estimated according to the method described by (Bates et al. 1973). Total phenolic compounds were carried out according to that method described by (Dai et al. 1993). Total soluble carbohydrates in yield were estimated according to the method of (Umbriet et al. 1959).
Assays of enzymes activities and lipid peroxidation
Extraction of enzymes was according to (Mukherjee and Choudhuri 1983). Superoxide dismutase (SOD) activities were estimated using the method of (Marklund and Marklund 1974). Catalase (CAT) activities were assayed according to the methods of (Aebi 1983). Peroxidase (POX) activities were determined according to the method of (Bergmeyer and Bernt 1974), The activities of polyphenol oxidase (PPO) were determined using the methods of (Kar and Mishra 1976). Lipid peroxidation was assayed as malondialdehyde (MDA) content in fresh leaves of Jute mallow according to that method described by (Heath and Packer 1968).
Determination of plant heavy metals contents
Heavy metals contents in the different samples of studied plants (edible plant part) were determined according to (Parkinson and Allen 1975). At the atomic spectroscopy laboratory, arid land agricultural research and services center, faculty of agriculture, Ain Shams University, Cairo, Egypt.
Statistical analysis
Statistical calculations were carried out using the computer programs SPSS version 25, Minitab version 19, and Microsoft Excel version 365 at 0.05 level of probability. The analysis of variance, one-way ANOVA, and post hoc Tukey’s test were used to analyse quantitative data with a parametric distribution. The confidence interval was set to 95% and the margin of error accepted was set to 5%.
Results
Biosynthesis and characterization of Se NPs
The production of Se NPs by GSH was observed in this study by a change in solution color to dark red which operated as a reducing agent or capping agent to reduce selenious acid into Se NPs and stabilize them in colloidal form. Filtrates of the prepared GSH were screened for their capacity for Se NPs biosynthesis. The color of the GSH seemed light, which shifted to extreme red concerning the exhibition of Se NP. The spectra exhibited the experimental peak (Fig. 1) conducting the O. D. (0.546; diluted 10 times), and the Se NPs were small in size, which was observed at 340 nm, according to the UV-Vis. analyses. Additionally, the detected peaks at 305 nm in the Se NPs spectrum corresponded to the GSH constituents. HRTEM image represented the spheroidal forms with moderately mono-dispersed Se NPs with a common size from 9.85 to 11.82 nm. The mean diameter was calculated to be 10.46 nm, as shown in Fig. 2a. The delivered mono-dispersed Se NPs were appointed to the prepared GSH; that was supposed to reduce, stabilize, and preserve agents (El-Batal et al. 2013, 2017, 2018). The DLS technique defined the typical particle size distribution and was estimated as 45.2. The polydispersity index (PDI) can be received from DLS devices or are distinguished from electron micrographs. International standards organizations (ISOs) have demonstrated that PDI values less than 0.05 are more expected to monodisperse models. In contrast, values more than 0.7 are expected to a polydisperse diffusion of particles. Herein, for the accepted PDI values (Fig. 2b), we found that the PDI value was 0.451. The surface features and morphology of the synthesized Se NPs were investigated using SEM images. Figure 3 depicts showed the SEM image of Se NPs biosynthesized by the GSH with variable boundary size and the equivalent spherical formation located within the GSH. Additionally, the SEM image of Se NPs incorporated with GSH, exhibits uniform NPs surfaces, and the surface appearance was clear. It can be detected that Se NPs were isolated typically as a rounded particle (Fig. 3a) across the GSH, which shows as a brilliant NPs combined and stabilized with GSH. EDX study was used to establish the basic structure of the biosynthesized Se NPs and the purity, as described in Fig. 3b. Se NPs exhibited specific absorption peaks of selenium element at 1.40 keV. The lack of other elemental peaks and a massive quantity of selenium in the spectra validate the selenium element purity. The appearance of carbon, oxygen, and chlorine peaks in the synthesized samples was due to the capping and stabilizing GSH, while the presence of Na peak was due to the application of NaOH in the preparation step. EDX examination of the prepared Se NPs by GSH (Fig. 3b) represented the essential peaks that correspond to the basic atoms found in GSH (O, C, Na, K, and Cl). XRD studies were exhibited in Fig. 4 for Se NPs synthesized by using GSH. XRD for the biogenic Se NPs describes the crystal and amorphous compositions for the precursor (GSH) and the synthesized Se NPs. It must be noted 2Ɵ at 22.21o (donated as *) corresponds to the amorphous kind of GSH. The results of the XRD study of the biosynthesized Se NPs in (Fig. 3) displayed the diffraction characteristics regarding 2Ɵ at 29.89o, 32.31o, 46.14o, 56.76o, and 66.94o, which represented the Bragg’s reflections at (100), (101), (111), (201) and (210), respectively. Finally, there is only one amorphous peak at 24.09o (donated as *) for GSH (Fig. 4) involved in the synthesis and stability of Se NPs. On the other hand, the average crystallite size of the biosynthesized Se NPs was determined by using the Williamson-Hall (W-H) equation (Belavi et al. 2012; Ashour et al. 2018; Maksoud et al. 2018; Pal et al. 2018; Abdel Maksoud et al. 2019), and was observed to be 18.59 nm according to the Eq. 1.
Where DW-H is the average crystallite size, β is the full-width at half maximum, λ is the X-ray wavelength and θ is the Bragg’s angle, k is a constant and ε is the strain of the samples.
Growth aspects
Lengths of shoots, roots, and number of leaves
The variations in growth parameters of carrot plants respond to wastewater irrigation as well as the use of selenium NPs alone or in combination with glycine betaine and proline are listed in (Fig. 5). Statistics revealed that when compared to control samples, wastewater irrigation dramatically improved all tested growth parameters. Furthermore, when compared to controls, treatment with selenium NPs alone or in combination with glycine betaine and proline had a positive effect on all growth parameters (Fig. 5). Concerning the impact of selenium NPs on the stressed plants, it was noticed that a single implementation with selenium NPs boosted shoot length, root length, and the number of leaves, respectively versus stressed plants. Plants treated with selenium NPs in combination with glycine betaine and proline have seen the greatest increases in shoots, root length, and number of leaves. Table 2 shows the effect of wastewater and the application of selenium NPs alone or in combination with glycine betaine and proline on the fresh and dry weights of shoots and roots in carrot plants. When compared to unstressed plants, carrot plants that were irrigated with wastewater showed a significant decrease in fresh and dry weight of shoots and roots (control). Contrarily, the application of selenium NPs combined with glycine betaine and proline alone or under stress conditions led to improvements in the fresh and dry weight of shoots and roots of carrot plants.
Photosynthetic pigments
The levels of chlorophyll content (chlorophyll a, chlorophyll b, chlorophyll a + b, and carotenoids) in stressed carrot plant leaf tissue were assessed to determine the protective roles of selenium NPs application on photosynthetic pigments under wastewater irrigation stress (Fig. 6). There was a decrease in chlorophyll a, chlorophyll b, chlorophyll a + b, and carotenoid content in stressed plants when compared to the untreated control sample (Fig. 6). When compared to only freshwater irrigated plants, selenium NPs application alone or in combination with glycine betaine and proline protected photosynthetic pigments from heavy metal-induced harmful effects, as evidenced by increased contents of chlorophyll a, chlorophyll b, chlorophyll a + b, and carotenoids in response to wastewater, respectively (Fig. 6). Non-stressed carrot plants treated with selenium NPs singly or combined with glycine betaine and proline also showed increased contents of chlorophyll a, chlorophyll b, chlorophyll a + b, and carotenoids, when compared with non-stressed control plants.
Organic solutes and secondary metabolites
The impact of wastewater irrigation and single or combined application of selenium NPs on the contents of total soluble carbohydrates in carrots shoots and roots are shown in Table 3. The total soluble carbohydrates content of carrot plants is significantly reduced due to wastewater irrigation. In terms of the interaction between wastewater irrigation and selenium NPs treatment, it was discovered that the application of selenium NPs combined with glycine betaine and proline was able to increase the contents of sugars in wastewater-stressed carrot plants when compared to untreated plants. The results presented in Table 3 show the effect of wastewater and selenium NPs on the soluble protein content of carrot plants. It was discovered that carrot plants irrigated with wastewater had a significantly lower content of soluble protein than unstressed plants (control). Conversely, applying selenium NPs individually or under stress conditions improved the soluble protein content of carrot plants. Table 3 summarizes the effects of wastewater stress, selenium NPs application alone or in combination with glycine betaine + proline, and their interactions on the content of free proline in carrot plants. The content of total phenol in carrot plant leaves under heavy metal stress and the application of selenium NPs were shown in Table 4. In this study, selenium NPs significantly increased phenol levels in heavy metal-stressed carrot plants, as well as osmolytes such as soluble protein, proline, and total soluble carbohydrates in shoots and roots (Table 4).
Antioxidant enzymes
The data analysis of our results indicated that wastewater irrigation and Se NPs application, alone or in combination with glycine, had a significant impact on the antioxidant enzyme activities of carrot plants (Table 5). When carrot plants were irrigated with wastewater, the activities of peroxidase (POD), superoxide dismutase (SOD), Catalase (CAT), and polyphenol oxidase (PPO) were significantly increased compared to unirrigated plants. Table 5 shows that when carrot plants are individually treated with selenium NPs, either alone or in combination with glycine betaine and proline, there are no significant changes in antioxidant enzyme activities when compared to controls (untreated plants) in normal conditions. Under wastewater stress conditions, selenium NPs either singly or combined with glycine betaine and proline exhibit a significant boost in the activities of antioxidant enzymes (POD, SOD, CAT, and PPO) in carrot plants when compared to control plants.
Stress biomarkers
Carrot plants grown under wastewater stress had higher levels of MDA and H2O2 compared to non-stressed controls (Fig. 7). This was the case throughout the two stages of carrot growth. Application of selenium NPs either singly or combined with glycine betaine and proline to wastewater-stressed plants played a pivotal role in minimizing the level of MDA and H2O2 compared with the plants exposed to wastewater stress only (Fig. 7). These effects are more pronounced in plants grown under wastewater stress and when selenium NPs combined with glycine betaine and proline are used especially at stage II of carrot growth. Under normal conditions carrot plants sprayed with selenium NPs showed decreasing in the level of MDA and H2O2 respectively, as compared with that of untreated control.
Yield aspects and biochemical measurements
Heavy metal stress reduced shoot length, root length, root diameter, fresh, dry weight of shoot, and fresh, dry weight of root significantly (Table 6). The use of selenium NPs in combination with glycine betaine and proline reduced the negative effects of heavy metal stress on carrot plant yield characteristics. Concerning the implementation of selenium NPs, the results presented in Table 6 show that when carrot plants are individually treated with selenium NPs in normal conditions, there is a significant increase in yield parameters when compared to controls (untreated plants). The osmolytes and β-Carotene contents of carrot yield is significantly declined in response to wastewater irrigation. With respect to the interaction between wastewater irrigation and selenium NPs treatment, it was shown that the application of selenium NPs combined with glycine betaine and proline was able to increase the contents of osmolytes and β-Carotene in wastewater-stressed carrot plants when compared to untreated plants (Table 7).
Accumulation of heavy metals in yield
The impact of wastewater stress, the selenium NPs singly or combined with glycine betaine and proline, and their interactions on the heavy metal accumulation in carrot plants are clarified in (Table 8). Under wastewater stress conditions carrot plants showed significant increases in Ni, Cd, Pb, and Co content when compared to control plants (Table 8). However, the application of selenium NPs, selenium NPs + glycine betaine, and proline resulted in a remarkable decrease in the Ni, Cd, Pb, and Co content when compared with stressed carrot plants (Table 8).
Discussion
Selenium is essential nutrient for humans and has many important biological functions, including immune regulation, antioxidant, antiviral, and anti-cancer properties. (Fairweather-Tait et al. 2011; Rayman 2012). Selenium is primarily supplied by eating cereal crops, but the content is low in most rice-producing countries in Asia and Africa. As per the World Health Organization (WHO), approximately 15% of the planet’s population is deficient in Selenium (Tan et al. 2018). So, increasing the selenium content of cereal crops with Se-containing fertilizer is essential to human health. Heavy metal stress had already been regarded as a significant issue in many terrestrial ecosystems worldwide. The industrialization has recently had a negative impact on soil and crop productivity by accumulating heavy metals. (Bashandy et al. 2020). The bioactive phytochemicals from plant extracts serve as a capping agent, preventing NPs aggregation and altering their biological activity (Fahimirad et al. 2019; Paiva-Santos et al. 2021). (Yilmaz et al. 2021), indicated that the pomegranate and watermelon peel extracts have a higher total phenolic content, and GSH which promotes in the reduction of silver ions to nanoscale-sized silver particles. The produced red color was assigned to the stimulation of biogenic Se NPs surface Plasmon alterations and provided a correct spectroscopic signal of their appearance (El-Batal et al. 2016; El-Ghazaly et al. 2017). The existence of filtrate peaks is shown by the UV-Vis spectrum of the produced GSH, which matches the literature studies (Akhtar et al. 2015; Nasiriboroumand et al. 2018). The intensity of the red color constructed was fitting to the power of the prepared GSH to biosynthesize Se NPs (El-Sayyad et al. 2020). Usually, surface plasmon resonance (SPR) is influenced by the intensity, dimension, morphological surfaces, structure and dielectric manners of the synthesized Se NPs (Kelly et al. 2003; Prasad and Selvaraj 2014; El-Baz et al. 2016; Attia et al. 2019).To investigate the average particle size and the desired shape of the biosynthesized Se NPs, HTEM images were performed, and its outcomes were compared with the DLS analysis, which operated to define particle size distribution, hydrodynamic radius, and polydispersity index (PDI) (El-Batal et al. 2016; El‐Baz et al. 2016; Elkodous et al. 2019). The current values demonstrate that the biosynthesized Se NPs was a moderate mono-size spread. The particle size distribution estimated by the DLS method was found to be greater than the average particle size determined by HRTEM images. Because of the large sizes of the biosynthesized Se NPs, the DLS process assessed the hydrodynamic radius that established close to the biosynthesized Se NPs and surrounded by water layers (El-Batal et al. 2014, 2016; El‐Baz et al. 2016; Hanora et al. 2016; Baraka et al. 2017), in the Se NPs biosynthesized by GSH, as shown in Fig. 2b. The EDX study is an analytic approach used for the elemental analysis or the chemical definition of the fabricated specimens (Baraka et al. 2017; Ashour et al. 2018; Maksoud et al. 2018; Elkodous et al. 2019). XRD analysis tested the crystal composition and the average crystal size of the synthesized Se NPs because it provides the state of the observed atoms (Ashour et al. 2018; Maksoud et al. 2018; Abdel Maksoud et al. 2019; Pal et al. 2019). In XRD results, all of the peaks matched the Joint Committee on Powder Diffraction Standards (JCPDS) of Se NPs with a standard card such as JCPDS File No 06-0362 (Prasad and Selvaraj 2014; Bai et al. 2017). This suggests that biogenic Se NPs crystallized in nature and formed the face-centered cubic (fcc) crystalline structure. Still, its intensity was shorter than that detected in GSH, and 2Ɵ was shifted due to the incorporation of Se NPs to the active GSH (El-Sayyad et al. 2020). Heavy metal stress has an impact on plant growth and metabolism, resulting in a decrease in most growth indices. Different studies have found a decrease in growth parameters as a result of heavy metal stress (Tiwari and Lata 2018). Our data are compatible with those of (Shahid et al. 2015; El-Shahir et al. 2021), who discovered that heavy metal stress significantly reduced plant height, root length, fresh and dry weights of shoots and roots. Heavy metal binds to the cell wall and the middle lamellae, enhancing pectin cross-linking and contributing to growth reduction, as well as to the plant as a whole (Khan et al. 2017). Furthermore, proline application improved the growth of chickpea plants grown under heavy metal stress (Hayat et al. 2013). Exogenous proline treatment resulted in better growth, photosynthetic efficiency, and antioxidant enzyme activity, all of which were associated with a higher yield. Selenium has been shown to boost the growth of stressed plants (Shekari et al. 2017; Shalaby et al. 2021). Moreover, (Soliman et al. 2022) proved that the applying selenium nanoparticles to wheat plants under stress or normal conditions significantly increased shoot lengths and root weights. Photosynthetic pigments are essential components of photosynthesis and play an important role in plant growth and yield attributes. In this study, heavy metal toxicity resulted in a significant decrease in chlorophyll a, chlorophyll b, and carotenoid concentrations in carrot plant leaves. Heavy metals also inhibit photosynthesis by disrupting the ultrastructure of chloroplasts and preventing the synthesis of essential pigments, inhibiting the Calvin cycle and the electron transport chain, and causing a carbon dioxide shortage by closing stomatal pores (Giannakoula et al. 2021). MDA and hydrogen peroxide accumulation could explain the decrease in photosynthetic pigments under heavy metal stress. The use of selenium nanoparticles in combination with glycine betaine and proline increased the content of photosynthetic pigments while eliminating the negative effects of heavy metal stress, as Se NPs could lower MDA and H2O2 levels in carrot leaves. This finding confirms the view of (Alnusairi et al. 2022), who mentioned that organic osmolytes, such as proline, are recognized to maintain cell water potential, safeguard macromolecules and enzymes from oxidative damage, increase enzyme activities, minimize H2O2 concentrations, and enhance tolerance of plants to oxidative stress conditions. The amino acid proline, which acts as an osmoprotectant, is more abundant in plants exposed to wastewater. The results also show that proline content significantly increased once carrot plants were irrigated with wastewater. In contrast, using the Se NPs under wastewater stress reduced the amount of proline in carrot plants compared to plants stressed by wastewater but not treated with the Se NPs (Table 5). Accordingly, proline levels in Se NPs-treated carrot plant leaves were significantly lower than in non-treated plants. Plants have developed organic solutes that regulate a variety of physiological processes in response to abiotic stress. In our experiment, the carrot plants under heavy metal stress showed higher levels of soluble carbohydrates, soluble protein, and total proline contents compared with non-stressed plants. Proline accumulation in plants may be responsible for preventing heavy metal-mediated lipid peroxidation and membrane alteration (Karakas et al. 2022). However, soluble protein, soluble carbohydrates, and free proline concentration were increased when the plants were treated with Se NPs. The increased accumulation of osmolytes has been documented to neutralize the toxic effect of metals as a result of Se NPs treatment (Zahedi et al. 2021). Exogenous application of Se NPs in combination with glycine betaine and proline to carrot plants increased total soluble carbohydrates, soluble protein, and proline, which is extremely sensitive to environmental stresses and controls the numerous genes involved in growth and metabolism by providing energy resources and carbon (Bano et al. 2021). Because of its hydrophilic nature, proline is active in chelating excess cytoplasmic metal ions, indicating a preference for coordinating nitrogen (N) or oxygen (O2) (Sofy et al. 2020). Proline can protect enzymes and biomolecules by acting as a protein stabilizer, metal chelator, and free radical scavenger (Boguszewska and Zagdańska 2012). When applied as a foliar spray, glycine betaine has been shown to increase endogenous levels of soluble protein and proline in a variety of plant species (Shafiq et al. 2021), implying that this chemical compound plays a critical role in increasing abiotic stress tolerance by managing the mechanisms involved in growth and yield production under stress conditions. Secondary metabolites, like phenols, as well facilitate osmoregulation and ROS scavenging, bolstering the enzymatic antioxidant system’s ability to withstand oxidative stress (Ali et al. 2021; Osman et al. 2021). Secondary metabolite synthesis is determined by a multitude of natural conditions, including biotic and abiotic stresses. (Ramakrishna and Ravishankar 2013; Abdel Latef et al. 2021). In this study, Se NPs significantly increased phenol levels in heavy metal-stressed carrot plants shoots. The increased accumulation of total phenols in carrot leaves reinforced the antioxidant system, preventing ROS cell damage to organelles. Regarding the application of selenium nanoparticles. In this line, (Zahedi et al. 2021) found an increase in secondary metabolite synthesis in plants in response to Se NPs application. Moreover, Soliman et al. (2022) found that Se NPs-induced phenol accumulation in wheat plants improved antioxidant efficiency and stress adaptation. Osmolytes as well scavenge ROS and mediate stress signaling for rapid elimination of stress responses to prevent oxidative damage. The prevention role of selenium nanoparticles may be related to the glycine betaine and proline combination with selenium particles. In this line, Hussain et al. (2020) illustrated that implementing glycine betaine to tobacco plants can initiate signaling pathways and induce stress resistance. The action of various enzymes such as CAT, SOD, and POD is thought to alleviate the disruption in cell homeostasis caused by ROS (Hussain et al. 2016; Ismail et al. 2022). The mechanism of ROS generation and scavenging by antioxidative capacity has been connected to plant tolerance to abiotic stresses (Sachdev et al. 2021). Under heavy metal stress conditions, the activities of enzymatic antioxidants (SOD, POD, and CAT) increased in carrot leaves, according to our findings. Similar findings in faba bean (Desoky et al. 2021) and Vicia faba L. Abid et al. (Abid et al. 2017) showed reduced oxidative stress caused by ROS generation. Exogenous application of Se NPs, particularly when combined with glycine betaine and proline, increased carrot antioxidant activity. Thus, improved carrot plant growth may be linked to increased antioxidant enzyme activity. Moreover, Soliman et al. (Soliman et al. 2022) found that foliar application of Se NPs increased antioxidant activity in wheat plants grown under abiotic stress conditions. Similarly, Sofy et al. (2020) mentioned that foliar application of proline modify antioxidant properties in maize plants, resulting in enhanced heavy metal stress tolerance. Excessive ROS accumulation in cell membranes compromises the structural integrity of key macromolecules such as proteins and lipids, affecting their normal development (Miller et al. 2008; Omer et al. 2022). Se NPs protected cell components from injury by absorbing excess ROS. This was accomplished by modifying antioxidant enzyme activities. Elevated lipid peroxidation and hydrogen peroxide have been noticed in heavy metal stressed plants as a consequence of unregulated ROS, which would be directly related to lipoxygenase activity (Ahanger et al. 2019; Attia et al. 2021) and could be inverted by adjustments such as Se alone or in combination with glycine betaine and proline (Zhou et al. 2020). As an outcome, in the current study, decreased ROS accumulation and oxidative damage in Se-treated carrot plants are likely associated with increased ROS-scavenging enzymes. Accordingly, plant stimulating with Se NPs increased detoxification of active oxygen species and lipid peroxidase produced by Cd stress, resulting in enhanced membrane stability and cell membrane structure prevention in Coriandrum sativum (Sardar et al. 2022; Zeeshan et al. 2023). Plant yield may be reduced due to a restriction in the growth of stressed carrot plants and a limitation in ROS scavenging compounds as a result of a reduction in photosynthetic pigments in carrot leaves. Our results are in line with those of other investigators (Badawy et al. 2021). Furthermore, the increased carrot yield caused by the use of selenium nanoparticles could be attributed to the stimulatory effect of glycine betaine and proline, which scavenges ROS and promotes the growth of stressed plants. Proline and glycine betaine have been shown in multiple research findings to reduce the effects of abiotic stress on plant growth and yield (Sofy et al. 2020; Shafiq et al. 2021). The ability of plant species to accumulate heavy metals varies greatly. These results indicate that carrot plants irrigated with wastewater accumulate higher metal concentrations, demonstrate higher plant allocation of the substrate metals in addition to interior plant mobility. These results are in line with (Fitzgerald et al. 2003; Nouri et al. 2009). Selenium NPs increased carrot plant ability to remediate toxic chemicals found in contaminated soil. The ameliorated effect of selenium nanoparticles is referred to the combination of proline and glycine betaine. Our results are following other investigators (Shafiq et al. 2021; Soliman et al. 2022) and (Sofy et al. 2020) who indicated that plants treated with proline showed a significant decrease in accumulated metals inside different plant parts.
Conclusion
Throughout the present study, selenium NPs alone or in combination with glycine betaine and proline ameliorated the negative impact of heavy metal problems generated by wastewater irrigation, resulting in significant increases in carrot plant growth traits, leaf pigments, soluble carbohydrates, protein contents, and yield criteria. Furthermore, as a mitigation method for the developed damage from heavy metal stress, using selenium NPs alone or in combination with glycine betaine and proline reduced the content of proline, malondialdehyde, and hydrogen peroxide while increasing the activities of carrot plant antioxidant enzymes. As a result, the current study suggests using selenium NPs, particularly when combined with glycine betaine and proline, to promote plant growth under normal and heavy metal stress conditions.
Data availability
All data and materials available.
References
Abdel Latef AAH, Omer AM, Badawy AA et al (2021) Strategy of salt tolerance and interactive impact of Azotobacter chroococcum and / or Alcaligenes faecalis inoculation on canola (Brassica napus L.) plants grown in saline soil. Plants 10:110. https://doi.org/10.3390/plants10010110
Abdel Maksoud MIA, El-ghandour A, El-Sayyad GS et al (2019) Incorporation of Mn2 + into cobalt ferrite via sol–gel method: insights on induced changes in the structural, thermal, dielectric, and magnetic properties. J Sol-Gel Sci Technol 90:631–642
Abid G, M’hamdi M, Mingeot D et al (2017) Effect of drought stress on chlorophyll fluorescence, antioxidant enzyme activities and gene expression patterns in faba bean (Vicia faba L). Arch Agron Soil Sci 63:536–552
Abu-Elela A, Elbehairy U, Abou-Hadid A (2021) Accumulation and risk assessment of heavy metals in vegetables irrigated with wastewater in giza governorate, Egypt. Arab Univ J Agric Sci 29:723–737
Adeel M, Ma C, Ullah S et al (2019) Exposure to nickel oxide nanoparticles insinuates physiological, ultrastructural and oxidative damage: a life cycle study on Eisenia fetida. Environ Pollut 254:113032
Aebi HE (1983) Catalase in vitro. Anal in methods in Enzymology. Elsevier, Amsterdam, p 105
Agoro MA, Adeniji AO, Adefisoye MA, Okoh OO (2020) Heavy metals in wastewater and sewage sludge from selected municipal treatment plants in Eastern Cape Province, South Africa. Water 12:2746
Ahanger MA, Qin C, Begum N et al (2019) Nitrogen availability prevents oxidative effects of salinity on wheat growth and photosynthesis by up-regulating the antioxidants and osmolytes metabolism, and secondary metabolite accumulation. BMC Plant Biol 19. https://doi.org/10.1186/s12870-019-2085-3
Akhtar S, Ismail T, Fraternale D, Sestili P (2015) Pomegranate peel and peel extracts: Chemistry and food features. Food Chem 174:417–425
Al-Zou’by JY, Al-Zboon KK, Al-Tabbal JA (2017) Low-cost treatment of grey water and reuse for irrigation of home garden plants. Environ Eng Manag J 16(2):351–359
Ali MM, Jeddi K, Attia MS et al (2021) Wuxal amino (Bio stimulant) improved growth and physiological performance of tomato plants under salinity stress through adaptive mechanisms and antioxidant potential. Saudi J Biol Sci 28:3204–3213
Ali S, Abbas Z, Seleiman MF et al (2020) Glycine betaine accumulation, significance and interests for heavy metal tolerance in plants. Plants 9:896
Alnusairi GSH, Soliman MH, KHAN AA et al (2022) Effects of EDTA and aqueous plants extract on the developmental and stress tolerance attributes of Spinacia oleracea and Brassica rapa under sewage water regime. Not Bot Horti Agrobot Cluj-Napoca 50:12534
Ashour AH, El-Batal AI, Maksoud MIAA et al (2018) Antimicrobial activity of metal-substituted cobalt ferrite nanoparticles synthesized by sol–gel technique. Particuology 40:141–151
APHA (1999) Standard methods for the examination of water and wastewater. Am Public Heal Assoc, Washington, DC, pp 541
Attia MS, El-Sayyad GS, Saleh SS et al (2019) Spirulina platensis-polysaccharides promoted green silver nanoparticles production using gamma radiation to suppress the expansion of pear fire blight-producing Erwinia amylovora. J Clust Sci 30:919–935
Attia MS, Osman MS, Mohamed AS et al (2021) Impact of foliar application of chitosan dissolved in different organic acids on isozymes, protein patterns and physio-biochemical characteristics of tomato grown under salinity stress. Plants 10:388
Badawy AA, Alotaibi MO, Abdelaziz AM et al (2021) Enhancement of seawater stress tolerance in barley by the endophytic fungus aspergillus ochraceus. Metabolites 11:428
Bai K, Hong B, He J et al (2017) Preparation and antioxidant properties of selenium nanoparticles-loaded chitosan microspheres. Int J Nanomedicine 12:4527
Bano I, Skalickova S, Sajjad H et al (2021) Uses of selenium nanoparticles in the plant production. Agronomy 11:2229
Baraka A, Dickson S, Gobara M et al (2017) Synthesis of silver nanoparticles using natural pigments extracted from Alfalfa leaves and its use for antimicrobial activity. Chem Pap 71:2271–2281
Bashandy SR, Abd-Alla MH, Dawood MFA (2020) Alleviation of the toxicity of oily wastewater to canola plants by the N2-fixing, aromatic hydrocarbon biodegrading bacterium Stenotrophomonas maltophilia-SR1. Appl Soil Ecol 154:103654
Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207
Belavi PB, Chavan GN, Naik LR et al (2012) Structural, electrical and magnetic properties of cadmium substituted nickel–copper ferrites. Mater Chem Phys 132:138–144
Bergmeyer HU, Bernt E (1974) UV-assay with pyruvate and NADH. Methods of enzymatic analysis. Elsevier, Amsterdam, pp 574–579
Boguszewska D, Zagdańska B (2012) ROS as signaling molecules and enzymes of plant response to unfavorable environmental conditions. Oxidative Stress Mech Biol Eff. InTech, Rijeka, 341–362
Dai GH, Andary C, Cosson-Mondolot L, Boubals D (1993) Polyphenols and resistance of grapevines to downy mildew. In: International Symposium on Natural Phenols in Plant Resistance 381, pp 763–766
Dar MI, Naikoo MI, Rehman F et al (2016) Proline accumulation in plants: roles in stress tolerance and plant development. Osmolytes and plants acclimation to changing environment: emerging omics technologies. Springer, Berlin, pp 155–166
Desoky E-SM, Mansour E, El-Sobky E-SEA et al (2021) Physio-biochemical and agronomic responses of faba beans to exogenously applied nano-silicon under drought stress conditions. Front Plant Sci 12:637783
Dias JS (2012) Major classes of phytonutriceuticals in vegetables and health benefits: a review. J Nutr Ther 1:31–62
El-Batal A, El-Baz AF, Abo Mosalam FM, Tayel AA (2013) Gamma irradiation induces silver nanoparticles synthesis by Monascus purpureus. J Chem Pharm Res 5:1–15
El-Batal AI, Al-Hazmi NE, Mosallam FM, El-Sayyad GS (2018) Biogenic synthesis of copper nanoparticles by natural polysaccharides and Pleurotus ostreatus fermented fenugreek using gamma rays with antioxidant and antimicrobial potential towards some wound pathogens. Microb Pathog 118:159–169
El-Batal AI, El-Sayed MH, Refaat BM, Askar AAZ (2014) Marine Streptomyces cyaneus strain Alex-SK121 mediated eco-friendly synthesis of silver nanoparticles using gamma radiation. Br J Pharm Res 4:2525
El-Batal AI, El-Sayyad GS, El-Ghamry A et al (2017) Melanin-gamma rays assistants for bismuth oxide nanoparticles synthesis at room temperature for enhancing antimicrobial, and photocatalytic activity. J Photochem Photobiol B Biol 173:120–139
El-Batal AI, Mosallam FM, Ghorab MM et al (2020) Factorial design-optimized and gamma irradiation-assisted fabrication of selenium nanoparticles by chitosan and pleurotus ostreatus fermented fenugreek for a vigorous in vitro effect against carcinoma cells. Int J Biol Macromol 156:1584–1599
El-Batal AI, Sidkey NM, Ismail AA et al (2016) Impact of silver and selenium nanoparticles synthesized by gamma irradiation and their physiological response on early blight disease of potato. J Chem Pharm Res 8:934–951
El-Ghazaly MA, Fadel N, Rashed E et al (2017) Anti-inflammatory effect of selenium nanoparticles on the inflammation induced in irradiated rats. Can J Physiol Pharmacol 95:101–110
El-Sayyad GS, El-Bastawisy HS, Gobara M, El-Batal AI (2020) Gentamicin-assisted mycogenic selenium nanoparticles synthesized under gamma irradiation for robust reluctance of resistant urinary tract infection-causing pathogens. Biol Trace Elem Res 195:323–342
El-Shahir AA, El-Tayeh NA, Ali OM et al (2021) The Effect of endophytic Talaromyces pinophilus on growth, absorption and accumulation of heavy metals of Triticum aestivum grown on sandy soil amended by sewage sludge. Plants 10:2659
El-Baz AF, El‐Batal AI, Abomosalam FM et al (2016) Extracellular biosynthesis of anti‐Candida silver nanoparticles using Monascus purpureus. J Basic Microbiol 56:531–540
Elgharably A, Mohamed HM (2016) Heavy metals uptake by wheat, bean and onion and characterization of microorganisms in a long-term sewage wastewater treated soil. Egypt J Soil Sci 56:605–620
Elkodous MA, El-Sayyad GS, Mohamed AE et al (2019) Layer-by-layer preparation and characterization of recyclable nanocomposite (CoxNi1 – xFe2O4; X = 0.9/SiO2/TiO2). J Mater Sci Mater Electron 30:8312–8328
Fahimirad S, Ajalloueian F, Ghorbanpour M (2019) Synthesis and therapeutic potential of silver nanomaterials derived from plant extracts. Ecotoxicol Environ Saf 168:260–278
Fairweather-Tait SJ, Bao Y, Broadley MR et al (2011) Selenium in human health and disease. Antioxid Redox Signal 14:1337–1383
FAO/WHO (2019) Food standards programme codex alimentarius commission: Report of the 11th session of the codex committee on contaminants in foods. April 2017. Rio de Janeiro. Brazil 3–7. http://www.fao.org/fao-who-codexalimentarius/en/. Accessed 7 Nov 2019
FAO/WHO (2020) Codex alimentarius international food standards: general standard for contaminants and toxins in food and feed (CXS 193–1995). 2019. https://www.fao.org/fao-who-codexalimentarius/thematic-reas/contaminants/en/. Accessed 26 Jan 2020
Fitzgerald EJ, Caffrey JM, Nesaratnam ST, McLoughlin P (2003) Copper and lead concentrations in salt marsh plants on the Suir Estuary, Ireland. Environ Pollut 123:67–74
Giannakoula A, Therios I, Chatzissavvidis C (2021) Effect of lead and copper on photosynthetic apparatus in citrus (Citrus aurantium L.) plants. The role of antioxidants in oxidative damage as a response to heavy metal stress. Plants 10:155
Gupta N, Yadav KK, Kumar V et al (2019) Trace elements in soil-vegetables interface: translocation, bioaccumulation, toxicity and amelioration-a review. Sci Total Environ 651:2927–2942
Hanora A, Ghorab M, El-Batal AI, Mosalam FMA (2016) Synthesis and characterization of gold nanoparticles and their anticancer activity using gamma radiation. J Chem Pharm Res 8:405–423
Hayat S, Hayat Q, Alyemeni MN, Ahmad A (2013) Proline enhances antioxidative enzyme activity, photosynthesis and yield of Cicer arietinum L. exposed to cadmium stress. Acta Bot Croat 72:323–335
Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
Hussain B, Lin Q, Hamid Y et al (2020) Foliage application of selenium and silicon nanoparticles alleviates cd and pb toxicity in rice (Oryza sativa L). Sci Total Environ 712:136497
Hussain S, Khan F, Cao W et al (2016) Seed priming alters the production and detoxification of reactive oxygen intermediates in rice seedlings grown under sub-optimal temperature and nutrient supply. Front Plant Sci 7:439
Iorizzo M, Ellison S, Senalik D et al (2016) A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution. Nat Genet 48:657–666
Ismail LM, Soliman MI, Abd El-Aziz MH, Abdel-Aziz HMM (2022) Impact of silica ions and nano silica on growth and productivity of pea plants under salinity stress. Plants 11:494
Kar M, Mishra D (1976) Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant Physiol 57:315–319
Karakas FP, Sahin G, Turker AU, Verma SK (2022) Impacts of heavy metal, high temperature, and UV radiation exposures on Bellis perennis L.(common daisy): comparison of phenolic constituents and antioxidant potential (enzymatic and non-enzymatic). South Afr J Bot 147:370–379
Kelly KL, Coronado E, Zhao LL, Schatz GC (2003) The optical properties of metal nanoparticles: the influence of size, shape, and dielectric environment. J Phys Chem B 107:668–677
Khan MA, Khan S, Khan A, Alam M (2017) Soil contamination with cadmium, consequences and remediation using organic amendments. Sci Total Environ 601:1591–1605
Li J, Chen Y, Lu H, Zhai W (2021) Spatial distribution of heavy metal contamination and uncertainty-based human health risk in the aquatic environment using multivariate statistical method. Environ Sci Pollut Res 28:22804–22822
Lian J, Zhao L, Wu J et al (2020) Foliar spray of TiO2 nanoparticles prevails over root application in reducing cd accumulation and mitigating Cd-induced phytotoxicity in maize (Zea mays L). Chemosphere 239:124794
Liu Y, Wu T, White JC, Lin D (2021) A new strategy using nanoscale zero-valent iron to simultaneously promote remediation and safe crop production in contaminated soil. Nat Nanotechnol 16:197–205
Lowry OH (1951) Protein determination with the Folin phenol reagent. J Biol Chem 193:265–275
Maksoud MIAA, El-Sayyad GS, Ashour AH et al (2018) Synthesis and characterization of metals-substituted cobalt ferrite [Mx co (1-x) Fe2O4;(M = zn, Cu and Mn; x = 0 and 0.5)] nanoparticles as antimicrobial agents and sensors for Anagrelide determination in biological samples. Mater Sci Eng C 92:644–656
Maksoud MIAA, El-Sayyad GS, El-Khawaga AM et al (2020) Nanostructured mg substituted Mn-Zn ferrites: a magnetic recyclable catalyst for outstanding photocatalytic and antimicrobial potentials. J Hazard Mater 399:123000
Marklund S, Marklund G (1974) Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:469–474
Merwad A-EMA (2019) Influence of natural plant extracts in reducing soil and water contaminants. Sustain Agric Environ Egypt Part I Soil-Water-Food Nexus, pp 161–188
Miller G, Shulaev V, Mittler R (2008) Reactive oxygen signaling and abiotic stress. Physiol Plant 133:481–489
Mukherjee SP, Choudhuri MA (1983) Implications of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol Plant 58:166–170
Nasiriboroumand M, Montazer M, Barani H (2018) Preparation and characterization of biocompatible silver nanoparticles using pomegranate peel extract. J Photochem Photobiol B Biol 179:98–104
Nouri J, Khorasani N, Lorestani B et al (2009) Accumulation of heavy metals in soil and uptake by plant species with phytoremediation potential. Environ Earth Sci 59:315–323
Omer AM, Osman MS, Badawy AA (2022) Inoculation with Azospirillum brasilense and/or Pseudomonas geniculata reinforces flax (Linum usitatissimum) growth by improving physiological activities under saline soil conditions. Bot Stud 63:1–15
Osman MS, Badawy AA, Osman AI, Abdel Latef AAH (2021) Ameliorative impact of an extract of the halophyte Arthrocnemum macrostachyum on growth and biochemical parameters of soybean under salinity stress. J Plant Growth Regul 40:1245–1256. https://doi.org/10.1007/s00344-020-10185-2
Paiva-Santos AC, Herdade AM, Guerra C et al (2021) Plant-mediated green synthesis of metal-based nanoparticles for dermopharmaceutical and cosmetic applications. Int J Pharm 597:120311
Pal K, Elkodous MA, Mohan MLN (2018) CdS nanowires encapsulated liquid crystal in-plane switching of LCD device. J Mater Sci Mater Electron 29:10301–10310
Pal K, Sajjadifar S, Abd Elkodous M et al (2019) Soft, self-assembly liquid crystalline nanocomposite for superior switching. Electron Mater Lett 15:84–101
Pandey J, Verma RK, Singh S (2019) Suitability of aromatic plants for phytoremediation of heavy metal contaminated areas: a review. Int J Phytoremediation 21:405–418
Parkinson JA, Allen SE (1975) A wet oxidation procedure suitable for the determination of nitrogen and mineral nutrients in biological material. Commun Soil Sci Plant Anal 6:1–11
Prasad KS, Selvaraj K (2014) Biogenic synthesis of selenium nanoparticles and their effect on as (III)-induced toxicity on human lymphocytes. Biol Trace Elem Res 157:275–283
Ramakrishna A, Ravishankar GA (2013) Role of plant metabolites in abiotic stress tolerance under changing climatic conditions with special reference to secondary compounds. Clim Chang Plant Abiotic Stress Toler, 705–726
Rayman MP (2012) Selenium and human health. Lancet 379:1256–1268
Sachdev S, Ansari SA, Ansari MI et al (2021) Abiotic stress and reactive oxygen species: Generation, signaling, and defense mechanisms. Antioxidants 10:277
Sardar R, Ahmed S, Shah AA, Yasin NA (2022) Selenium nanoparticles reduced cadmium uptake, regulated nutritional homeostasis and antioxidative system in Coriandrum sativum grown in cadmium toxic conditions. Chemosphere 287:132332
Shafiq S, Akram NA, Ashraf M et al (2021) Influence of glycine betaine (natural and synthetic) on growth, metabolism and yield production of drought-stressed maize (Zea mays L.) plants. Plants 10:2540
Shahid M, Khalid S, Abbas G et al (2015) Heavy metal stress and crop productivity. Crop production and global environmental issues. Springer, Berlin, pp 1–25
Shalaby SE, Al-Balakocy NG, Beliakova MK, Othman AM (2021) Effect of wet processing operations on the functional properties imparted to polyester fabrics loaded with different metal oxides NPs part II: effect of the different sequences of dyeing. J Eng Fiber Fabr. https://doi.org/10.1177/15589250211005760
Shekari Z, Zare HR, Falahati A (2017) Developing an impedimetric aptasensor for selective label–free detection of CEA as a cancer biomarker based on gold nanoparticles loaded in functionalized mesoporous silica films. J Electrochem Soc 164:B739
Sofy MR, Seleiman MF, Alhammad BA et al (2020) Minimizing adverse effects of pb on maize plants by combined treatment with jasmonic, salicylic acids and proline. Agronomy 10:699
Soliman MH, Alnusairi GSH, Khan AA et al (2022) Biochar and selenium nanoparticles induce water transporter genes for sustaining carbon assimilation and grain production in salt-stressed wheat. J Plant Growth Regul. https://doi.org/10.1007/s00344-022-10636-y
Tan LC, Nancharaiah YV, van Hullebusch ED, Lens PNL (2018) Selenium: environmental significance, pollution, and biological treatment technologies. Anaerob treat mine wastewater Remov selenate its co-contaminants. CRC Press, Florida, pp 9–71
Tiwari S, Lata C (2018) Heavy metal stress, signaling, and tolerance due to plant-associated microbes: an overview. Front Plant Sci 9:452
Umbriet WW, Burris RH, Stauffer JF et al (1959) Monometric technique, a manual description method, applicable to study of desiring metabolism. Burgess Publishing Company, Plano, p 239
Usman M, Farooq M, Wakeel A et al (2020) Nanotechnology in agriculture: current status, challenges and future opportunities. Sci Total Environ 721:137778
Wang Y, Jiang F, Ma C et al (2019) Effect of metal oxide nanoparticles on amino acids in wheat grains (Triticum aestivum) in a life cycle study. J Environ Manage 241:319–327
Yaqvob M, Golale A, Masoud S, Hamid RG (2011) Influence of different concentration of heavy metals on the seed germination and growth of tomato. Afr J Environ Sci Technol 5:420–426
Yilmaz MT, Ispirli H, Taylan O, Dertli E (2021) A green nano-biosynthesis of selenium nanoparticles with Tarragon extract: structural, thermal, and antimicrobial characterization. LWT 141:110969
Zahedi SM, Hosseini MS, Daneshvar Hakimi Meybodi N, Peijnenburg W (2021) Mitigation of the effect of drought on growth and yield of pomegranates by foliar spraying of different sizes of selenium nanoparticles. J Sci Food Agric 101:5202–5213
Zaki AG, Hasanien YA, El-Sayyad GS (2022) Novel fabrication of SiO2/Ag nanocomposite by gamma irradiated Fusarium oxysporum to combat Ralstonia solanacearum. AMB Express 12:1–18
Zeeshan M, Hu YX, Guo XH et al (2023) Physiological and transcriptomic study reveal SeNPs-mediated AsIII stress detoxification mechanisms involved modulation of antioxidants, metal transporters, and transcription factors in Glycine max L.(Merr.) Roots. Environ Pollut 317:120637
Zhou P, Adeel M, Shakoor N et al (2020) Application of nanoparticles alleviates heavy metals stress and promotes plant growth: an overview. Nanomaterials 11:26
Acknowledgements
The authors would like to thank the Botany and Microbiology Department, Faculty of Science, Al-Azhar University and Nanotechnology Research Unit (P.I. Prof. Dr. Ahmed I. El-Batal), Drug Microbiology Lab., Drug Radiation Research Department, NCRRT, EAEA, Cairo, Egypt, for promoting this research.
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El-Batal, A.I., Ismail, M.A., Amin, M.A. et al. Selenium nanoparticles induce growth and physiological tolerance of wastewater‑stressed carrot plants. Biologia 78, 2339–2355 (2023). https://doi.org/10.1007/s11756-023-01401-x
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DOI: https://doi.org/10.1007/s11756-023-01401-x