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Spirulina platensis-Polysaccharides Promoted Green Silver Nanoparticles Production Using Gamma Radiation to Suppress the Expansion of Pear Fire Blight-Producing Erwinia amylovora

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Abstract

The present research examined the influence of eco-friendly silver nanoparticles (Ag NPs) synthesized by Spirulina platensis-polysaccharides and gamma rays on the growth of bacterial plant pathogen which caused pear fire blight. Plant pathogen was isolated from blighted blossoms, leaves, and thin slices of cankerous branches of the pear and identified both biochemically and genetically as Erwinia amylovora. Silver nitrate was mixed with S. platensis-polysaccharide and used for the biogenic Ag NPs biosynthesis under the influence of gamma radiation. The synthesized Ag NPs was characterized by UV–Vis., HRTEM, DLS, XRD, FTIR, SEM, EDX, and mapping analysis. Data received from HRTEM and DLS calculated the average particles size of the spherical Ag NPs and was found to be 25.25 nm. FTIR analysis determined a polysaccharide which extracted from S. platensis and effective for the reduction of Ag+. Ag NPs exhibited antibacterial potential against E. amylovora (17.0 mm ZOI), while Ag+ possesses activity about 8.0 mm ZOI, and S. platensis filtrate possesses no activity against the tested bacteria. Owing to the different characteristics of the biogenic Ag NPs as purity, antibacterial activity and green eco-friendly method, they may be applied in the agriculture field as pure, safe and new nanomaterial-based treatment.

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Acknowledgements

The authors would like to thank the Nanotechnology Research Unit (P.I. Prof. Dr. Ahmed I. El-Batal), Drug Microbiology Lab., Drug Radiation Research Department, NCRRT, Egypt, for financing and supporting this study under the project “Nutraceuticals and Functional Foods Production by using Nano/Biotechnological and Irradiation Processes”. Also, the authors would like to thank Prof. Mohamed Gobara (Professor at Military Technical College, Egyptian Armed Forces), Dr. Muhamed I. Abdel Maksoud (Lecturer at NCRRT), and Zeiss microscope team in Cairo for their invaluable advice during this study.

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Authors and Affiliations

  1. Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, 11884, Egypt

    Mohamed S. Attia

  2. Drug Radiation Research Department, Biotechnology Division, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Cairo, Egypt

    Gharieb S. El-Sayyad & Ahmed I. El-Batal

  3. Horticulture Research Institute, Agricultural Research Center (ARC), Giza, Egypt

    Sherif S. Saleh

  4. Plant Pathology Research Institute, Agricultural Research Center (ARC), Giza, Egypt

    Naglaa M. Balabel

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  1. Mohamed S. Attia
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Attia, M.S., El-Sayyad, G.S., Saleh, S.S. et al. Spirulina platensis-Polysaccharides Promoted Green Silver Nanoparticles Production Using Gamma Radiation to Suppress the Expansion of Pear Fire Blight-Producing Erwinia amylovora. J Clust Sci 30, 919–935 (2019). https://doi.org/10.1007/s10876-019-01550-7

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