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Transcriptome profiling of unigenes participating in salt tolerance in purslane (Portulaca oleracea) under salinity stress

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Abstract

The second generation of RNA-sequencing was done on the leaves of salt-tolerant (‘PL’) and salt-sensitive (‘LCL’) purslane (Portulaca oleracea) genotypes. Twenty-four RNA libraries were obtained at four different time intervals (0 h, 1 h, 24 h, and 72 h) during salinity stress. Compared with control, at 1 h, 24 h, and 72 h of 200 mM NaCl treatment, 7407, 2499, and 7597 of differential expression genes (DEGs) were qualified in ‘LCL’, and 8861, 7974, and 10349 in ‘PL’ the Kyoto Encyclopedia of Genes and Genomes (KEGG)- and Gene Ontology-based pathway studies displayed that salt stress response of purslane was a complex metabolic regulation process. Two kinds of analyses, especially KEGG, showed that salt stress affected gene expression linked with metabolism of fatty acid. Two fatty acid metabolism pathways, “α-linolenic acid metabolism” and “fatty acid degradation” were downregulated in two genotypes at 24 h vs 0 h and 72 h vs 0 h of NaCl stress. Another fatty acid metabolism pathway, “biosynthesis of unsaturated fatty acids”, was only upregulated at 24 h vs 0 h and 72 h vs 0 h of NaCl stress in ‘PL’. Upregulated expressions of FAD2, SAD, ACX2, and CLKR27 in “biosynthesis of unsaturated fatty acids” pathway might be associated with salt tolerance of ‘PL’ in purslane. Among these, upregulation of FAD2 and SAD might be beneficial to the accumulation of polyunsaturated fatty acids, upregulation of ACX2 might promote the plant growth under salt stress in salt-tolerant purslane genotype. The results are of great significance for further screening salt-tolerant and high-quality purslane genotypes.

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Data availability

The datasets generated during the current study are available from the corresponding author on reasonable request.

Abbreviations

ACX2:

Acyl-coenzyme A oxidase 2

ALA:

α-Linolenic acid

CLKR27:

3-Oxoacyl-[acyl-carrier-protein] reductase

DEGs:

Differential expression genes

ER:

Endoplasmic reticulum

FAD:

Fatty acid desaturase

FPKM:

Fragments per kilobase of transcript per million mapped reads

GO:

Gene ontology

GLA:

γ-Linolenic acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

KEGG:

Kyoto Encyclopedia of Genes and Genomes

LA:

Linoleic acid

PUFA:

Polyunsaturated fatty acid

RWC:

Relative water content

SAD:

Stearoyl-ACP desaturase

UFA:

Unsaturated fatty acid

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Acknowledgements

The present study was funded by the National Key Technology R&D Program under grant number 2015BAL02B01. We are thankful to Novo gene Co., China, which provided technical assistance in data analysis and RNA-sequencing.

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  1. School of Design, Shanghai Jiao Tong University, Shanghai, 200240, China

    Hongmei Du, Shuiqingqing Hu & Shengquan Che

  2. School of Agricultural and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Shah Zaman

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11738_2023_3535_MOESM1_ESM.xlsx

Figure S1–S6. The GO Directed Acyclic Graph graphical (DAG) output of enriched biological process (BP) (Figure S 1A), cellular component (CC) (Figure S 1B) and molecular function (MF) (Figure S 1C) in differential expression genes (DEGs) of LC_L1 vs LC_L0; BP (Figure S 2A) and MF (Figure S 2B) in DEGs of LC_L24 vs LC_L0; BP (Figure S 3A), CC (Figure S 3B) and MF (Figure S 3C) in DEGs of LC_L72 vs LC_L0; BP (Figure S 4A), CC (Figure S 4B) and MF (Figure S 4C) in DEGs of P_L1 vs P_L0; BP (Figure S 5A) and MF (Figure S 5B) in DEGs of P_L24 vs P_L0; BP (Figure S 6A), CC (Figure S 6B) and MF (Figure S 6C) in DEGs of P_L72 vs P_L0. Figure S7-S8. KEGG pathways enrichment analysis of down-regulated genes in LC_L1 vs LC_L0 (Figure S 7A), LC_L24 vs LC_L0 (Figure S 7B) and LC_L72 vs LC_L0 (Figure S 7C) of ‘LCL’, and P_L1 vs P_L0 (Figure 8A), P_L24 vs P_L0 (Figure 8B) and P_L72 vs P_L0 (Figure 8C) of ‘PL’. Figure S9-S10 KEGG pathways enrichment analysis of up-regulated genes LC_L1 vs LC_L0 (Figure S 9A), LC_L24 vs LC_L0 (Figure S 9B) and LC_L72 vs LC_L0 (Figure S 9C) of ‘LCL’, and P_L1 vs P_L0 (Figure 10A), P_L24 vs P_L0 (Figure 10B) and P_L72 vs P_L0 (Figure 10C) of ‘PL’ (XLSX 4867 kb)

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Supplementary file4 (XLSX 22 kb)

Supplementary file5 (XLSX 13 kb)

Supplementary file6 (PPT 92 kb)

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Du, H., Zaman, S., Hu, S. et al. Transcriptome profiling of unigenes participating in salt tolerance in purslane (Portulaca oleracea) under salinity stress. Acta Physiol Plant 45, 76 (2023). https://doi.org/10.1007/s11738-023-03535-6

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