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The influence of the cooling of donor cultures on the in vitro adventitious regeneration and carbohydrate metabolism of four dwarfing apple rootstocks

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Abstract

We examined the effects of cooling applied for 4 to 20 weeks on donor cultures of four dwarfing apple rootstocks (P16, P22, P59 and M26). Our aim includes increasing their competence for in vitro adventitious shoot regeneration from the leaves. Donor cultures were maintained on a shoot multiplication medium at 4°C in the dark for 4 months, followed by subculture on a fresh medium for 4 weeks. The cooling of the cultures caused an increase in the adventitious shoot number and a decrease in the starch content and an increase in the soluble sugar content (monosaccharides, raffinose and stachyose). The accumulation of stachyose in response to cold is a new observation, and it suggests that raffinose and stachyose play important role in the acclimation of dwarf apple rootstocks to low temperatures.

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Abbreviations

BAP:

6-Benzylaminopurine

IAA:

Indole-3-acetic acid

MS:

Murashige and Skoog medium

NAA:

Alfa-napthaleneacetic acid

TDZ:

Thidiazuron

WPM:

Woody plant medium

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Acknowledgments

Authors are grateful to Mrs. Lucyna Ogórek for technical help. This study was financed by grant 580/E-177/SPB of the Polish Ministry of Sciences and High Education.

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Authors and Affiliations

  1. Research Institute of Pomology and Floriculture, P. O. Box 105, 96-100, Skierniewice, Poland

    Teresa Orlikowska, Marta Zawadzka & Danuta Kucharska

  2. Department of Plant Physiology and Biotechnology, University of Warmia and Mazury, Oczapowskiego 1A, 10-719, Olsztyn, Poland

    Lesław B. Lahuta

Authors
  1. Teresa Orlikowska
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  2. Marta Zawadzka
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  3. Danuta Kucharska
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  4. Lesław B. Lahuta
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Correspondence to Teresa Orlikowska.

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Communicated by J. Pauk.

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Orlikowska, T., Zawadzka, M., Kucharska, D. et al. The influence of the cooling of donor cultures on the in vitro adventitious regeneration and carbohydrate metabolism of four dwarfing apple rootstocks. Acta Physiol Plant 32, 333–340 (2010). https://doi.org/10.1007/s11738-009-0411-3

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  • DOI: https://doi.org/10.1007/s11738-009-0411-3

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