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Influence of gibberellic acid on auxin biosynthesis and their effects on coleoptile elongation in garlic

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Abstract

To demonstrate the effect of auxin on intact coleoptile growth, garlic (Allium sativum L.) cloves were inoculated in agar supplemented with DW (control), GA3 and GA3+tryptophan (a precursor of IAA, GA3+T). The coleoptiles were harvested at 24 h intervals to measure growth in terms of length, activities of IAAld DH (which convert tryptophan to IAA) and peroxidase (that oxidizes IAA). Contents of endogenous IAA and PAA were also measured by indirect ELISA. Peroxidase activity was suppressed by GA3 treatment and increased by GA3+T treatment. Although endogenous contents of IAA were increased by the addition of GA3 and even more by GA3+T in the media, there was no further increase in coleoptile length, suggesting that garlic coleoptiles are sufficient in their production of IAA.

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Abbreviations

BSA:

Bovine serum albumin

DEAE cellulose:

Diethylaminoethyl cellulose

DMF:

Dimethyl formamide

DW:

Distilled water

EDTA:

Ethylene diamine tetra acetic acid

GA3 :

Gibberellic acid3

IAA:

Indole-3-acetic acid

IAAld DH:

Indole acetaldihyde dehydrogenase

PAA:

Phenyl acetic acid

T:

Tryptophan

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Authors and Affiliations

  1. Department of Biosciences, Saurashtra University, 360 005, Rajkot, India

    S. J. Sata, S. J. Gokani & V. S. Thaker

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  1. S. J. Sata
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  2. S. J. Gokani
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  3. V. S. Thaker
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Correspondence to V. S. Thaker.

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Sata, S.J., Gokani, S.J. & Thaker, V.S. Influence of gibberellic acid on auxin biosynthesis and their effects on coleoptile elongation in garlic. Acta Physiol Plant 24, 393–398 (2002). https://doi.org/10.1007/s11738-002-0035-3

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  • DOI: https://doi.org/10.1007/s11738-002-0035-3

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