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Radioimmunoassay for pmol-quantities of indole-3-acetic acid for use with highly stable [125I]- and [3H]IAA derivatives as radiotracers

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Abstract

A radioimmunoassay for the detection of as little as 0.5–1 pmol indole-3-acetic acid (IAA) in unpurified or partially purified plant extracts is described. The assay makes use of either IAA[125I]tyrosine methyl ester or [3H]IAA methyl ester as radioactive antigens and IAA methyl ester as the assay standard (measuring range: 1–200 pmol). Levels of extractable IAA in a number of biological samples have been estimated.

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Abbreviations

BSA:

bovine serum albumin

2,4-D:

2,4-dichlorophenoxy acetic acid

DMF:

dimethyl formamide

GC-MS:

gas chromatography-mass spectroscopy

IAA:

indole-3-acetic acid

RIA:

radioimmunoassay

SICM:

selected ion current monitoring

TLC:

thin layer chromatography

TME:

tyrosine methyl ester

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Authors and Affiliations

  1. Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, Postfach 10 21 48, D-4630, Bochum, Federal Republic of Germany

    Elmar W. Weiler

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  1. Elmar W. Weiler
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Part 18 in the series: “Use of immunoassay in plant science”

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Weiler, E.W. Radioimmunoassay for pmol-quantities of indole-3-acetic acid for use with highly stable [125I]- and [3H]IAA derivatives as radiotracers. Planta 153, 319–325 (1981). https://doi.org/10.1007/BF00384250

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  • DOI: https://doi.org/10.1007/BF00384250

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