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Preparation and characterization of enzyme-responsive zwitterionic nanoparticles for monoclonal antibody delivery

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Abstract

Monoclonal antibodies have been used in many diseases, but how to improve their delivery efficiency is still a key issue. As the modification of zwitterionic polymers can maintain the stability and biological activity of monoclonal antibodies, in this study, zwitterionic monomers, sulfobetaine methacrylate (SBMA), and 3-[[2-(methacryloyloxy) ethyl] dimethylammonio] propionate (CBMA) were used to prepare monoclonal antibody-loaded zwitterionic nanoparticles with the aid of the crosslinker of MMP-2 enzyme-responsive peptide which was a rapid synthesis process under mild conditions. The results from dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) indicated that a series of zwitterionic nanoparticles had been successfully prepared by the in situ free radical polymerization using the MMP-2 enzyme-responsive peptide as the cross-linking agent. These nanoparticles were spherical with the sizes of (18.7±1.9) nm (SBMA nanoparticle) and (18.2±2.1) nm (CBMA nanoparticle), and the surface contained zwitterionic polymers. It was revealed that they had no cytotoxicity, could be released in tumor microenvironment by enzyme to inhibit the growth of tumor cells, and was able to effectively penetrate endothelial cells (> 2%) by transwell. Therefore, the development of this strategy has a great prospect for the delivery of monoclonal antibodies.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (Grant No. 51773151) and the Tianjin Applied Basic Research Multi-Input Fund (21JCYBJC01560). Thank Miss Yiou Lyu for her help in article writing and editing.

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Authors and Affiliations

  1. Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin, 300350, China

    Changxin Hou, Huike Xing & Xubo Yuan

  2. School of Materials Science and Engineering, Tianjin University, Tianjin, 300350, China

    Changxin Hou, Huike Xing & Xubo Yuan

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  1. Changxin Hou
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  2. Huike Xing
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Correspondence to Xubo Yuan.

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Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Hou, C., Xing, H. & Yuan, X. Preparation and characterization of enzyme-responsive zwitterionic nanoparticles for monoclonal antibody delivery. Front. Mater. Sci. 17, 230667 (2023). https://doi.org/10.1007/s11706-023-0667-3

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  • DOI: https://doi.org/10.1007/s11706-023-0667-3

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