Abstract
Currently, there is great interest worldwide in the use of plant-mediated green technologies to minimize the harmful effects caused by known traditional methods in the manufacture of nanoparticles. Therefore, the objective of this study was to develop a facile, eco-friendly, and cost-effective green approach for the synthesis of silver nanoparticles (Ag-NPs) using Ajuga iva extract. The synthesized nanoparticles (NPs) were characterized firstly by a visual indicator, in which the color of mixture will change from pale yellow to dark brown under optimal synthesis conditions. After that, the characterization was continued by ultraviolet–visible (UV–Vis) spectroscopy and scanning electron microscopy coupled with energy-dispersive spectrometry (SEM–EDX). The obtained results reveal that Ag-NPs were polygonal, with sizes ranging from 100 to 300 nm. Their characteristic peak absorbance was situated around 450 nm. The EDX analysis reveals a high percentage of silver atoms, which confirms the synthesis of silver NPs. Moreover, the FTIR study revealed that the Ag-NPs were capped by biomolecules. The in vitro toxicity activity, evaluated on model immune cells, showed an absence of any significant toxicity at the concentrations used since they did not affect either the cell viability of splenocytes and thymocytes, and the biological activity of B-lymphocytes. In addition, the photocatalytic activity of these biosynthesized NPs was studied against methylene blue, under solar irradiation where they showed an efficiency of 48.6% after 90 min of exposure. All those results make Ag-NPs synthesized by Ajuga iva a promising candidate for various biological and industrial applications.
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Ahmed MJ, Murtaza G, Mehmood A, Bhatti TM (2015) Green synthesis of silver nanoparticles using leaves extract of Skimmia laureola: characterization and antibacterial activity. Mater Lett 153:10–13. https://doi.org/10.1016/j.matlet.2015.03.143
Alharbi NS, Alsubhi NS (2022) Green synthesis and anticancer activity of silver nanoparticles prepared using fruit extract of Azadirachta indica. J Radiat Res Appl Sci 15:335–345. https://doi.org/10.1016/j.jrras.2022.08.009
Allali H, Benmehdi H, Dib AM, Tabti B, Ghalem S, Benabadji N (2008) Phytotherapy of diabetes in West Algeria. Asian J Chem
Arshad H, Sadaf S, Hassan U (2022) De-novo fabrication of sunlight irradiated silver nanoparticles and their efficacy against E. coli and S. epidermidis. Sci Rep 12:676. https://doi.org/10.1038/s41598-021-04674-x
Baghiani A, Boumerfeg S, Adjadj M, Ameni D, Djermouni M, Khelifi-Touhami F, Charef N, Khennouf S, Arrar L (2011) Antioxidants, free radicals scavenging and xanthine oxidase inhibitory potentials of Ajuga iva L. Extracts Free Radic Antioxid 1:21–30. https://doi.org/10.5530/ax.2011.4.5
Bakrim A, Ngunjiri J, Crouzet S, Guibout L, Balducci C, Girault J-P, Lafont R (2014) Ecdysteroid profiles of two Ajuga species, A. iva and A. remota. Nat Prod Commun 9:1934578X1400900. https://doi.org/10.1177/1934578X1400900804
Baruah D, Yadav RNS, Yadav A, Das AM (2019) Alpinia nigra fruits mediated synthesis of silver nanoparticles and their antimicrobial and photocatalytic activities. J Photochem Photobiol B 201:111649. https://doi.org/10.1016/j.jphotobiol.2019.111649
Bellakhdar J, Claisse R, Fleurentin J, Younos C (1991) Repertory of standard herbal drugs in the Moroccan pharmacopoea. J Ethnopharmacol 35:123–143. https://doi.org/10.1016/0378-8741(91)90064-K
Bennaghmouch L, Hajjaji N, Gmira N (2002) Flavonoïdes D’Ajuga iva (L.) Schreb (Labiée). Actes Inst Agron Vet Maroc
Bhattacharya D, Gupta RK (2005) Nanotechnology and potential of microorganisms. Crit Rev Biotechnol 25:199–204. https://doi.org/10.1080/07388550500361994
Boukir A, Guiliano M, Asia L, El Hallaoui A, Mille G (1998a) A fraction to fraction study of photo-oxidation of BAL 150 crude oil asphaltenes. Analusis 26:358–364. https://doi.org/10.1051/analusis:1998185
Boukir A, Aries E, Guiliano M, Asia L, Doumenq P, Mille G (2001) Subfractionation, characterization and photooxidation of crude oil resins. Chemosphere 43:279–286. https://doi.org/10.1016/S0045-6535(00)00159-4
Boukir A, Fellak S, Doumenq P (2019a) Structural characterization of Argania spinosa Moroccan wooden artifacts during natural degradation progress using infrared spectroscopy (ATR-FTIR) and X-Ray diffraction (XRD). Heliyon 5:e02477. https://doi.org/10.1016/j.heliyon.2019.e02477
Boukir A, Mehyaoui I, Fellak S, Asia L, Doumenq P (2019Bb) The effect of the natural degradation process on the cellulose structure of Moroccan hardwood fiber: a survey on spectroscopy and structural properties. Mediterr J Chem 8:179–190. https://doi.org/10.13171/mjc8319050801ab
Boukir A, Guiliano M, Doumenq P, Mille G (1998a) Caractérisation structurale d’asphaltènes pétroliers par spectroscopic infrarouge (IRTF) : application à la photo-oxydation
Bouramdane Y, Fellak S, El Mansouri F, Boukir A (2022) Impact of natural degradation on the aged lignocellulose fibers of Moroccan cedar softwood: structural elucidation by infrared spectroscopy (ATR-FTIR) and X-ray diffraction (XRD). Fermentation 8:698. https://doi.org/10.3390/fermentation8120698
Daoudi A, El Youbi AEH, Bagrel D, Aarab L (2013) In vitro anticancer activity of some plants used in Moroccan traditional medicine. Vitro Anticancer Act Some Plants Used Moroc Tradit Med. https://doi.org/10.5897/JMPR12.1167
De M, Ghosh PS, Rotello VM (2008) Applications of nanoparticles in biology. Adv Mater 20:4225–4241. https://doi.org/10.1002/adma.200703183
Durán N, Marcato PD, De Souza GIH, Alves OL, Esposito E (2007) Antibacterial effect of silver nanoparticles produced by fungal process on textile fabrics and their effluent treatment. J Biomed Nanotechnol 3:203–208. https://doi.org/10.1166/jbn.2007.022
El Youbi AE, Bousta D, Ouahidi I, Aarab L (2010) Criblage pharmacologique primaire d’une plante endémique originaire du Sud Marocain (Tetraena gaetula [Emb. & Maire] Beier & Thulin). C R Biol 333:736–743. https://doi.org/10.1016/j.crvi.2010.08.001
El-Hilaly J, Lyoussi B, Wibo M, Morel N (2004) Vasorelaxant effect of the aqueous extract of Ajuga iva in rat aorta. J Ethnopharmacol 93:69–74. https://doi.org/10.1016/j.jep.2004.03.020
Elshafei AM, Othman AM, Elsayed MA, Al-Balakocy NG, Hassan MM (2021) Green synthesis of silver nanoparticles using Aspergillus oryzae NRRL447 exogenous proteins: optimization via central composite design, characterization and biological applications. Environ Nanotechnol Monit Manag 16:100553. https://doi.org/10.1016/j.enmm.2021.100553
Filip GA, Moldovan B, Baldea I, Olteanu D, Suharoschi R, Decea N, Cismaru CM, Gal E, Cenariu M, Clichici S, David L (2019) UV-light mediated green synthesis of silver and gold nanoparticles using Cornelian cherry fruit extract and their comparative effects in experimental inflammation. J Photochem Photobiol B 191:26–37. https://doi.org/10.1016/j.jphotobiol.2018.12.006
Gardea-Torresdey JL, Tiemann KJ, Gamez G, Dokken K, Tehuacanero S, José-Yacamán M (1999) Gold nanoparticles obtained by bio-precipitation from gold(III) solutions. J Nanoparticle Res 1:397–404. https://doi.org/10.1023/A:1010008915465
Gour A, Jain NK (2019) Advances in green synthesis of nanoparticles. Artif Cells Nanomed Biotechnol 47:844–851. https://doi.org/10.1080/21691401.2019.1577878
Guiliano M, Boukir A, Doumenq P, Mille G, Crampon C, Badens E, Charbit G (2000) Supercritical fluid extraction of bal 150 crude oil asphaltenes. Energy Fuels 14:89–94. https://doi.org/10.1021/ef9900435
Gurunathan S, Kalishwaralal K, Vaidyanathan R, Venkataraman D, Pandian SRK, Muniyandi J, Hariharan N, Eom SH (2009) Biosynthesis, purification and characterization of silver nanoparticles using Escherichia coli. Colloids Surf B Biointerfaces 74:328–335. https://doi.org/10.1016/j.colsurfb.2009.07.048
Hajji L, Boukir A, Assouik J, Kerbal A, Kajjout M, Doumenq P, De Carvalho ML (2015) A multi-analytical approach for the evaluation of the efficiency of the conservation-restoration treatment of Moroccan historical manuscripts dating to the 16th, 17th, and 18th centuries. Appl Spectrosc 69:920–938. https://doi.org/10.1366/14-07688
Hajji L, Boukir A, Assouik J, Pessanha S, Figueirinhas JL, Carvalho ML (2016) Artificial aging paper to assess long-term effects of conservative treatment. Monitoring by infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), and energy dispersive X-ray fluorescence (EDXRF). Microchem J 124:646–656. https://doi.org/10.1016/j.microc.2015.10.015
Hilaly JE, Lyoussi B (2002) Hypoglycaemic effect of the lyophilised aqueous extract of Ajuga iva in normal and streptozotocin diabetic rats. J Ethnopharmacol 80:109–113. https://doi.org/10.1016/S0378-8741(01)00407-X
Isaac RSR, Sakthivel G, Murthy Ch (2013) Green synthesis of gold and silver nanoparticles using Averrhoa bilimbi fruit extract. J Nanotechnol 2013:1–6. https://doi.org/10.1155/2013/906592
Israili ZH, Lyoussi B (2009) Ethnopharmacology of the plants of genus Ajuga. Pak J Pharm Sci 22:425–462
Jadoun S, Arif R, Jangid NK, Meena RK (2021) Green synthesis of nanoparticles using plant extracts: a review. Environ Chem Lett 19:355–374. https://doi.org/10.1007/s10311-020-01074-x
Jain S, Mehata MS (2017) Medicinal plant leaf extract and pure flavonoid mediated green synthesis of silver nanoparticles and their enhanced antibacterial property. Sci Rep 7:15867. https://doi.org/10.1038/s41598-017-15724-8
Joseph S, Mathew B (2015) Microwave-assisted green synthesis of silver nanoparticles and the study on catalytic activity in the degradation of dyes. J Mol Liq 204:184–191. https://doi.org/10.1016/j.molliq.2015.01.027
Kahrilas GA, Wally LM, Fredrick SJ, Hiskey M, Prieto AL, Owens JE (2014) Microwave-assisted green synthesis of silver nanoparticles using orange peel extract. ACS Sustain Chem Eng 2:367–376. https://doi.org/10.1021/sc4003664
Khalil MMH, Ismail EH, El-Baghdady KZ, Mohamed D (2014) Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity. Arab J Chem 7:1131–1139. https://doi.org/10.1016/j.arabjc.2013.04.007
Khan I, Saeed K, Khan I (2019) Nanoparticles: properties, applications and toxicities. Arab J Chem 12:908–931. https://doi.org/10.1016/j.arabjc.2017.05.011
Kredy MH (2018) The effect of pH, temperature on the green synthesis and biochemical activities of silver nanoparticles from Lawsonia inermis extract. J Pharm Sci Res
Kumara Swamy M, Sudipta KM, Jayanta K, Balasubramanya S (2015) The green synthesis, characterization, and evaluation of the biological activities of silver nanoparticles synthesized from Leptadenia reticulata leaf extract. Appl Nanosci 5:73–81. https://doi.org/10.1007/s13204-014-0293-6
Li S, Shen Y, Xie A, Yu X, Qiu L, Zhang L, Zhang Q (2007) Green synthesis of silver nanoparticles using Capsicum annuum L. extract. Green Chem 9:852. https://doi.org/10.1039/b615357g
Liu W-T (2006) Nanoparticles and their biological and environmental applications. J Biosci Bioeng 102:1–7. https://doi.org/10.1263/jbb.102.1
Llorent-Martínez EJ, Zengin G, Ortega-Barrales P, Zakariyyah Aumeeruddy M, Locatelli M, Mollica A, Mahomoodally MF (2019) Characterization of the phytochemical profiles and biological activities of Ajuga chamaepitys subsp. chia var. chia and Ajuga bombycina by high-performance liquid chromatography-electrospray ionization–tandem mass spectrometry (HPLC–ESI–MS n ). Anal Lett 52:852–868. https://doi.org/10.1080/00032719.2018.1500581
Marsoul A, Boukir A, Ijjaali M, Taleb M, Arrousse N, Salim R, Dafali A (2023) Phytochemical characterization, antioxidant proprieties and electrochemical investigations of methanolic extract of Rubia t.L. roots for LC-steel corrosion protection in 1 M HCl medium. J Bio Tribo Corros 9:32. https://doi.org/10.1007/s40735-023-00749-6
Mohamed El-Rafie H, Abdel-Aziz Hamed M (2014) Antioxidant and anti-inflammatory activities of silver nanoparticles biosynthesized from aqueous leaves extracts of four Terminalia species. Adv Nat Sci Nanosci Nanotechnol 5:035008. https://doi.org/10.1088/2043-6262/5/3/035008
Mortazavi-Derazkola S, Zinatloo-Ajabshir S, Salavati-Niasari M (2015) Novel simple solvent-less preparation, characterization and degradation of the cationic dye over holmium oxide ceramic nanostructures. Ceram Int 41:9593–9601. https://doi.org/10.1016/j.ceramint.2015.04.021
Mousavi B, Tafvizi F, Zaker Bostanabad S (2018) Green synthesis of silver nanoparticles using Artemisia turcomanica leaf extract and the study of anti-cancer effect and apoptosis induction on gastric cancer cell line (AGS). Artif Cells Nanomed Biotechnol 46:499–510. https://doi.org/10.1080/21691401.2018.1430697
Narayanan KB, Sakthivel N (2010) Biological synthesis of metal nanoparticles by microbes. Adv Colloid Interface Sci 156:1–13. https://doi.org/10.1016/j.cis.2010.02.001
Nouri A, Tavakkoli Yaraki M, Lajevardi A, Rezaei Z, Ghorbanpour M, Tanzifi M (2020) Ultrasonic-assisted green synthesis of silver nanoparticles using Mentha aquatica leaf extract for enhanced antibacterial properties and catalytic activity. Colloid Interface Sci Commun 35:100252. https://doi.org/10.1016/j.colcom.2020.100252
Osuntokun J, Onwudiwe DC, Ebenso EE (2019) Green synthesis of ZnO nanoparticles using aqueous Brassica oleracea L. var. italica and the photocatalytic activity. Green Chem Lett Rev 12:444–457. https://doi.org/10.1080/17518253.2019.1687761
Parashar UK, Saxena PS, Srivastava A (2009) Bioinspired synthesis of silver nanoparticles. Dig J Nanomater Biostructures
Ramar K, Vasanthakumar V, Priyadharsan A, Priya P, Raj V, Anbarasan PM, Vasanthakumari R, Jafar Ahamed A (2018) Green synthetic approach of silver nanoparticles from Bauhinia tomentosa Linn. leaves extract for potent photocatalytic and in vitro biological applications. J Mater Sci Mater Electron 29:11509–11520. https://doi.org/10.1007/s10854-018-9246-2
Rana A, Yadav K, Jagadevan S (2020) A comprehensive review on green synthesis of nature-inspired metal nanoparticles: mechanism, application and toxicity. J Clean Prod 272:122880. https://doi.org/10.1016/j.jclepro.2020.122880
Raut Rajesh W, Lakkakula Jaya R, Kolekar Niranjan S, Mendhulkar Vijay D, Kashid Sahebrao B (2009) Phytosynthesis of silver nanoparticle using Gliricidia sepium (Jacq.). Curr Nanosci 5:117–122. https://doi.org/10.2174/157341309787314674
Ravichandran V, Sumitha S, Ning CY, Xian OY, Kiew Yu U, Paliwal N, Shah SA, Tripathy M (2020) Durian waste mediated green synthesis of zinc oxide nanoparticles and evaluation of their antibacterial, antioxidant, cytotoxicity and photocatalytic activity. Green Chem Lett Rev 13:102–116. https://doi.org/10.1080/17518253.2020.1738562
Roda E, Bottone M, Biggiogera M, Milanesi G, Coccini T (2019) Pulmonary and hepatic effects after low dose exposure to nanosilver: early and long-lasting histological and ultrastructural alterations in rat. Toxicol Rep 6:1047–1060. https://doi.org/10.1016/j.toxrep.2019.09.008
Saidi S, Remok F, Handaq N, Drioiche A, Gourich AA, Menyiy NE, Amalich S, Elouardi M, Touijer H, Bouhrim M, Bouissane L, Nafidi H-A, Bin Jardan YA, Bourhia M, Zair T (2023) Phytochemical profile, antioxidant, antimicrobial, and antidiabetic activities of Ajuga iva (L.). Life 13:1165. https://doi.org/10.3390/life13051165
Saxena J, Sharma PK, Sharma MM, Singh A (2016) Process optimization for green synthesis of silver nanoparticles by Sclerotinia sclerotiorum MTCC 8785 and evaluation of its antibacterial properties. Springerplus 5:861. https://doi.org/10.1186/s40064-016-2558-x
Sharma VK, Yngard RA, Lin Y (2009) Silver nanoparticles: green synthesis and their antimicrobial activities. Adv Colloid Interface Sci 145:83–96. https://doi.org/10.1016/j.cis.2008.09.002
Singh A, Kaur K (2020) Biological and physical applications of silver nanoparticles with emerging trends of green synthesis. In: Marius Avramescu S, Akhtar K, Fierascu I, Bahadar Khan S, Ali F, Asiri A (eds) Engineered nanomaterials: health and safety. IntechOpen, London. https://doi.org/10.5772/intechopen.88684
Stensberg MC, Wei Q, McLamore ES, Porterfield DM, Wei A, Sepúlveda MS (2011) Toxicological studies on silver nanoparticles: challenges and opportunities in assessment, monitoring and imaging. Nanomed 6:879–898. https://doi.org/10.2217/nnm.11.78
Tang J, Xiong L, Wang S, Wang J, Liu L, Li J, Wan Z, Xi T (2008) Influence of silver nanoparticles on neurons and blood-brain barrier via subcutaneous injection in rats. Appl Surf Sci 255:502–504. https://doi.org/10.1016/j.apsusc.2008.06.058
Tippayawat P, Phromviyo N, Boueroy P, Chompoosor A (2016) Green synthesis of silver nanoparticles in aloe vera plant extract prepared by a hydrothermal method and their synergistic antibacterial activity. PeerJ 4:e2589. https://doi.org/10.7717/peerj.2589
Ulug B, Haluk Turkdemir M, Cicek A, Mete A (2015) Role of irradiation in the green synthesis of silver nanoparticles mediated by fig (Ficus carica) leaf extract. Spectrochim Acta A Mol Biomol Spectrosc 135:153–161. https://doi.org/10.1016/j.saa.2014.06.142
Vaezi M (2022) In silico and in vitro studies of naturally occurring tyrosinase inhibitors: structure–activity relationship. Chem Afr 5:1873–1887. https://doi.org/10.1007/s42250-022-00466-6
Vaezi M (2023a) Efficacy and biomedical roles of unsaturated fatty acids as bioactivefood components. Curr Chem Biol 17:79–90. https://doi.org/10.2174/2212796817666230222103441
Vaezi M (2023b) Structure and inhibition mechanism of some synthetic compounds and phenolic derivatives as tyrosinase inhibitors: review and new insight. J Biomol Struct Dyn 41:4798–4810. https://doi.org/10.1080/07391102.2022.2069157
Vanaja M, Paulkumar K, Baburaja M, Rajeshkumar S, Gnanajobitha G, Malarkodi C, Sivakavinesan M, Annadurai G (2014) Degradation of methylene blue using biologically synthesized silver nanoparticles. Bioinorg Chem Appl 2014:1–8. https://doi.org/10.1155/2014/742346
Vidhu VK, Philip D (2014) Catalytic degradation of organic dyes using biosynthesized silver nanoparticles. Micron 56:54–62. https://doi.org/10.1016/j.micron.2013.10.006
Ziyyat A, Legssyer A, Mekhfi H, Dassouli A, Serhrouchni M, Benjelloun W (1997) Phytotherapy of hypertension and diabetes in oriental Morocco. J Ethnopharmacol 58:45–54. https://doi.org/10.1016/S0378-8741(97)00077-9
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NM and LA conceptualized the work, performed the experiments mentioned and analyzed the data. NM drafted the first version of the manuscript. AB contributed to the analysis of the FTIR spectrum. SL and IO contributed to the revision of the manuscript. LA supervised the study. All authors have read and approved the final version of the manuscript.
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Al Moudani, N., Laaraj, S., Ouahidi, I. et al. Green synthesis of silver nanoparticles using leaves extract of Ajuga iva: characterizations, toxicity and photocatalytic activities. Chem. Pap. 78, 1505–1516 (2024). https://doi.org/10.1007/s11696-023-03177-5
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DOI: https://doi.org/10.1007/s11696-023-03177-5