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Isolation of secondary metabolites of two endemic species: Salvia rosifolia Sm. and Salvia cerino-pruinosa Rech. f. var. elazigensis (Lamiaceae)

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Abstract

In the present study, activity-guided isolation and structural elucidation of antioxidant secondary metabolites of ethanol extracts prepared from the root and aerial parts of Salvia rosifolia and Salvia cerino-pruinosa var. elazigensis species was aimed. The ethanol extracts of the species were fractionated and the antioxidant capacities of the fractions were determined by DPPH-free radical and ABTS-cation radical scavenging activity, and cupric-reducing antioxidant capacity (CUPRAC) assays. Isolation studies of the fractions with high antioxidant activities were carried out. Thirty nine secondary metabolites of known structure were isolated from the active extracts. 17 of the isolated compounds are in phenolic-flavonoid structure, 4 of them are in fatty acid structure, 7 of them are in abietane type diterpene structure, 2 of which are paraquinone and 5 of which are in aromatic structure, 8 of them are in triterpene structure, 2 of which are ursane, 2 of which are oleane and 4 of which are lupane structure, and 3 of them are in steroid structure. The structures of the isolated compounds were determined by UV, IR, 1H- and 13C-NMR-(APT), HMQC-HMBC, LCMS-IT/TOF and GC-MS techniques. It was determined that phenolic compounds among the isolated compounds were more active than terpenoid compounds in all three methods.

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Acknowledgements

This work was supported by TUBITAK (The Scientific and Technological Research Council of Turkey) (Project number: KBAG 114Z801).

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Authors and Affiliations

  1. Department of Pharmacognosy, Faculty of Pharmacy, Dicle University, 21280, Diyarbakir, Turkey

    Esra Yaris, Leyla Balur Adsız, Eyyup Tuncay & Abdulselam Ertas

  2. Department of Analytical Chemistry, Faculty of Pharmacy, Dicle University, 21280, Diyarbakir, Turkey

    Ismail Yener

  3. Dicle University Science and Technology Research and Application Center (DUBTAM), 21280, Diyarbakir, Turkey

    Mustafa Abdullah Yilmaz

  4. The Council of Forensic Diyarbakir Group Chairmanship, 21100, Diyarbakir, Turkey

    Mehmet Akdeniz

  5. Deparment of Chemistry, Faculty of Science, Mugla Sitki Kocman University, 48121, Mugla, Turkey

    Erhan Kaplaner

  6. Department of Biology, Faculty of Education, Van Yuzuncu Yil University, 65080, Van, Turkey

    Mehmet Firat

  7. Department of General and Analytical Chemistry, Faculty of Pharmacy, Istanbul University, 34116, Istanbul, Turkey

    Ufuk Kolak

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  1. Esra Yaris
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Yaris, E., Balur Adsız, L., Yener, I. et al. Isolation of secondary metabolites of two endemic species: Salvia rosifolia Sm. and Salvia cerino-pruinosa Rech. f. var. elazigensis (Lamiaceae). Food Measure 15, 4929–4938 (2021). https://doi.org/10.1007/s11694-021-01065-8

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