Abstract
The present study was designed to investigate the effect of microencapsulated Lactobacillus rhamnosus (L. rhamnosus) for aroma and other quality characteristics of Turkish dry-fermented sausage (sucuk). L. rhamnosus was microencapsulated with an optimal coating material combination and used in sucuk production as a probiotic culture. The samples were stored for a 2-, 4-, and 6-month period. After each storage period, physicochemical, microbiological, aroma, and sensorial properties of sucuk with microencapsulated L. rhamnosus were compared with the control sample (L. plantarum) and sucuk with free L. rhamnosus. At the end of the 6-month storage, the viability of the probiotic cell showed an approximately 1.0 log cfu/g reduction in sucuk with free L. rhamnosus and 0.20 log cfu/g reduction in sucuk with microencapsulated L. rhamnosus. The sucuk aromatic extracts were obtained by a Likens-Nickerson distillation and extraction system and analyzed by GC-MS. A total of 60 aroma compounds, including six aldehydes, nine acids, five alcohols, four esters, three volatile phenols, two sulphur compounds, and 31 terpenes were identified and quantified in the samples. The concentration of total aroma compounds was found to be between 9964.5 and 45135.3 µg/kg. Except for the 6-month storage treatment, the highest total amounts of aroma compounds were found in sucuk with microencapsulated L. rhamnosus followed by free L. rhamnosus and the control sample. It can be concluded that a novel sucuk production with microencapsulated L. rhamnosus probiotic had better quality in terms of overall aroma properties.
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References
H. Bozkurt, O. Erkmen, Effects of some commercial additives on the quality of sucuk (turkish dry-fermented sausage). Food Chem. 101, 1465–1473 (2007)
G. Kaban, Geleneksel olarak üretilen sucuklardan laktik asit bakterileri ile katalaz pozitif kokların izolasyonu-identifikasyonu, üretimde kullanılabilme imkanları ve uçucu bileşikler üzerine etkileri. Atatürk Üniversitesi, Fen Enstitüsü, Gıda Mühendisliği Anabilim Dalı, Doktora Tezi (PhD thesis), 101s, (2007)
H. Bozkurt, O. Erkmen, Effects of starter cultures and additives on the quality of turkish style sausages (sucuk). Meat Sci. 61, 149–156 (2002)
L. De Vuyst, G. Falony, F. Leroy, Probiotics in fermented sausages. Meat Sci. 80, 75–78 (2008)
B. Ergönül, Farklı probiyotik kültürler kullanılarak hindi sucuğu üretimi ve kalite üzerine etkileri. Celal Bayar Üniversitesi, FenBilimleri Enstitüsü, Doktora Tezi (PhD thesis), (2009)
M. Sidira, P. Kandylis, M. Kanellaki, Y. Kourkoutas, Effect of immobilized Lactobacillus casei on the evolution of flavor compounds in probiotic dry-fermented sausages during ripening. Meat Sci. 100, 41–51 (2015)
Ü. Çiçek, N. Polat, Investigation of physicochemical and sensorial quality of a type of traditional meat product: bez sucuk. LWT Food Sci. Technol. 65, 145–151, (2016)
C. Montanari, E. Bargossi, A. Gardini, R. Lanciotti, R. Magnani, F. Faustogardini, G. Tabanelli, Correlation between volatile profiles of italian fermented sausages and their size and starter culture. Food Chem. 192, 736–744 (2016)
A. De Prisco, G. Mauriello, Probiotication of foods: a focus on microencapsulation tool. Trends Food Sci. Technol. 48, 27–39 (2016)
P. Muthukumarasamy, R.A. Holley, Microbiological and sensory quality of dry fermented sausages containing alginate-microencapsulated Lactobacillus reuteri. Int. J. Food Microbiol. 111, 164–169 (2006)
Y.R. Espinoza, Y.G. Navarro, Non-dairy probiotic products. Food Microbiol. 27, 1–11 (2010)
E. Unal, Probiyotik Laktik Asit Bakterilerinden L. rhamnosus’un Fermente Sucuklarin Bazi Özellikleri Üzerine Etkisi. Çukurova Üniversitesi, Ziraat Fakültesi, Gıda Mühendisliği Bölümü, Doktora Tezi, (PhD thesis), (2014)
M. Chen, K. Chen, Y. Kuo, Optimal thermotolerance of Bifidobacterium bifidum in gellan–alginate microparticles. Biotechnol. Bioeng. 98(2), 411–419 (2007)
H.Y. Gokalp, Starter kültür kullanılarak türk tipi sucuk imalinde metod geliştirilmesi. Türkiye Bilimsel Ve Teknik Araştırma Kurumu, Veterinerlik ve Hayvancılık Araştırma Grubu, Proje No:Vhag-523, Erzurum, (1982)
H. Gençcelep, Sucuk üretiminde değişik starter kültürler ve farklı nitrit seviyelerinin biyojen amin oluşumu üzerine etkileri. Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, Doktora Tezi (PhD thesis), 131s, (2006)
W.F. Harrigan, Laboratory Methods in Food Microbiology, 3rd edn. (Academic Press Ltd., USA, 1998)
C. Bujalance, M. Jiménez-Valera, E. Moreno, A. Ruiz-Bravo, A selective differential medium for Lactobacillus plantarum. J. Microbiol. Methods 66, 572–575 (2006)
N. Tharmaraj, N.P. Shah, Selective enumeration of Lactobacillus delbrueckii ssp. bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, Bifidobacteria, Lactobacillus casei, Lactobacillus rhamnosus and propionibacteria. J. Dairy Sci. 86, 2288–2296 (2003)
D. Ansorena, O. Gimeno, I. Astiasaran, J. Bello, Analysis of volatile compounds by G-MS of a dry fermented sausage: chorizo de pamplona. Food Res. Int. 34, 67–75 (2001)
M.T. Ayseli, G. Filik, S. Selli, Evaluation of volatile compounds in chicken breast meat using simultaneous distillation and extraction with odour activity value. J. Food Nutr. Res. 53(2), 137–142 (2014)
Y. Bek, E. Efe, Araştırma ve Deneme Metodlar. C.U. Ziraat Fakültesi Ders Kitabı, Adana, (1988)
TSE Turkish sucuk, Turkish Standard, TS 1070, ICS 67.120.10. Ankara. (2002)
K. Śmiecińska, N. Hnatyk, T. Daszkiewicz, D. Kubiak, P. Matusevičius, The effect of frozen storage on the quality of vacuum-packaged Turkey meat. Vet Med Zoot. 71, 61–66, (2015)
A. Serteser, V. Gök, Antioksidan kullanımının fermente sucuklarda bazı kalite özellikleri üzerine etkileri. Tübitak Hızlı Destek Projesi, Proje No: 104O230,121s, (2006)
M.A. Çakir, Isıl işlem uygulamasının sucuğun uçucu bileşikleri ve diğer kalitatif özelliklerine etkisi. Atatürk Üniversitesi, FenBilimleri Ensititüsü, GıdaMühendisliğiAnabilim Dalı, Yüksek Lisans Tezi (MSc thesis), 54s, (2010)
L. Ekici, I. Ozturk, C.O. Karaman, F. Tornuk, O. Sagdic, H. Yetim, Effects of black carrot concentrateon some physicochemical, textural, bioactive, aroma and sensory properties of sucuk, a traditional Turkish dry-fermented sausage. LWT Food Sci. Technol. 62,718–726, (2015)
A.H. Çon, H.Y. Gökalp, Production of bacteriocin-like metabolites by lactic acid cultures isolated from sucuk samples. Meat Sci. 55, 89–96 (2000)
S. Fonseca, A. Cachaldora, M. Gómez, I. Franco, J. Carballo, Effect of different autochthonous starter cultures on the volatile compounds profile and sensory properties of galician chorizo, a traditional spanish dry fermented sausage. Food Control 33, 6–14 (2013)
I. Goubet, C. Dahout, E. Semon, E. Guichard, J.L. Le Quere, A. Voilley, Competitive binding of aroma compounds by α-cyclodextrin. J. Agric. Food Chem. 49, 5916–5922 (2001)
B.A. Santos, P.C.B. Campagnol, M.B. Fagundes, R. Wagner, M.A.R. Pollonio, Generation of volatile compounds in Brazilian low-sodium dry fermented sausages containing blends of NaC1, KC1, And CaC12 during processing and storage. Food Res. Int. 74, 306–314 (2015)
M. Janssens, N. Myter, L. De Vuyst, F. Leroy, Species diversity and metabolic impact of the microbiota are low in spontaneously acidified Belgian sausages with an added starter culture of Staphylococcus carnosus. Food Microbiol. 29, 167–177 (2012)
S.R. Moyano, A. Martín, M.J. Benito, E. Aranda, R. Casquete, M.G. Córdoba, Implantation ability of the potential probiotic strain, Lactobacillus reuteri Pl519, in “salchichón”, a traditional Iberian dry fermented sausage. J. Food Sci. 76(5), 268–275 (2011)
G. Kaban, Sucuk and pastırma: microbiological changes and formation of volatile compounds. Meat Sci. 95, 912–918 (2013)
M. Kargozaria, S. Moinia, A.A. Bastib, Z. Emam-Djomeha, M. Ghasemloud, I.R. Martine, H. Gandomib, A.A. Carbonell-Barrachina, A. Szumnyg, Development of Turkish dry-fermented sausage (sucuk) reformulated with camel meat and hump fat and evaluation of physicochemical, textural, fatty acid and volatile compound profiles during ripening. LWT Food Sci. Technol. 59, 849–858 (2014)
W. Sun, Q. Zhao, H. Zhao, M. Zhao, B. Yang, Volatile compounds of cantonese sausage released at different stages of processing and storage. Food Chem. 121, 319–325 (2010)
S.R. Moyano, A. Martin, M.J. Benito, A. Hernández, R. Casquete, M.G. Córdoba, Application of Lactobacillus fermentum Hl57 and Pediococcus acidilactici Sp979 as potential probiotics in the manufacture of traditional Iberian dry-fermented sausages. Food Microbiol. 28(5), 839–847 (2011)
Acknowledgements
We thank the Scientific and Technical Research Council of Turkey (TUBITAK, Project 112O481) and Cukurova University Research Fund (ZF-2010-D17 and ZF2011BAP10) for financial supports. We also wish to thank Bryan Christiansen from Global Research Society, Indianapolis, Indiana 46214 - USA for his outstanding editing and proof reading.
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Turhan, E.U., Erginkaya, Z. & Selli, S. The effect of microencapsulated Lactobacillus rhamnosus and storage period on aroma properties of Turkish dry-fermented sausage (sucuk). Food Measure 11, 2131–2141 (2017). https://doi.org/10.1007/s11694-017-9597-z
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DOI: https://doi.org/10.1007/s11694-017-9597-z