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Development of a functional synbiotic beverage fortified with different cereal sprouts and prebiotics

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Abstract

The study was devoted to developing a novel synbiotic beverage based on with millet, rye and alfalfa sprouts with a mixed culture of Lactobacillus casei and Lactobacillus plantarum. In this regard, the influences of incorporated prebiotics inulin and oligofructose on probiotics viability during the refrigerated storage (4 ± 1 °C, 28 days) as well as under the simulated gastric condition were investigated. The characteristics such as microbial viability, physicochemical properties (viscosity, pH, titrable acidity and radical scavenging activity) and sensorial evaluation were assessed. The synbiotic beverage produced contained 108 CFU ml−1 for L. casei, with a good survival throughout the storage period (108 CFU ml−1) and L. plantarum at sufficient levels (106 CFU ml−1) after about 21 days. Inulin and oligofructose promoted the growth of the strains and their viability under cold storage while conferring higher sensory scores. In this context, the beverages demonstrated acceptable sensory attributes. The viability (bacterial survival) of over 55% for all the strains was achieved under simulated gastric condition. Therefore, the introduced fermented beverage was a good food matrix from the viability of probiotics as well as under the gastric condition and sensory characteristics

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References

  • Afshari R, Hosseini H, Khaksar R, Mohammadifar MA, Amiri Z, Komeili R, Khaneghah AM (2015) Investigation of the effects of inulin and β-glucan on the physical and sensory properties of low-fat beef burgers containing vegetable oils: optimisation of the formulation using D-optimal mixture design. Food Technol Biotechnol 53:436–445

    Article  CAS  Google Scholar 

  • AOAC (2010) Official methods of analysis of the association official analytical chemists. Association of Official Analytical Chemists, Arlington

    Google Scholar 

  • Cardarelli HR, Buriti FC, Castro IA, Saad SM (2008) Inulin and oligofructose improve sensory quality and increase the probiotic viable count in potentially synbiotic petit-suisse cheese. LWT-Food Sci Technol 41:1037–1046

    Article  CAS  Google Scholar 

  • Coda R, Rizzello CG, Trani A, Gobbetti M (2011) Manufacture and characterization of functional emmer beverages fermented by selected lactic acid bacteria. Food Microbiol 28:526–536

    Article  CAS  Google Scholar 

  • Cornara L, Xiao J, Burlando B (2016) Therapeutic potential of temperate forage legumes: a review. Crit Rev Food Sci Nutr 56:S149–S161

    Article  CAS  Google Scholar 

  • De Souza Oliveira RP, Rodrigues Florence AC, Perego P, De Oliveira MN, Converti A (2011) Use of lactulose as prebiotic and its influence on the growth, acidification profile and viable counts of different probiotics in fermented skim milk. Int J Food Microbiol 145:22–27

    Article  Google Scholar 

  • Ebrahimi B, Mohammadi R, Rouhi M, Mortazavian AM, Shojaee-Aliabadi S, Koushki MR (2018) Survival of probiotic bacteria in carboxymethyl cellulose-based edible film and assessment of quality parameters. LWT 87:54–60

    Article  CAS  Google Scholar 

  • El-Adawy TA (2002) Nutritional composition and antinutritional factors of chickpeas (Cicer arietinum L.) undergoing different cooking methods and germination. Plant Foods Hum Nutr 57:83–97

    Article  CAS  Google Scholar 

  • FAO/WHO (2006) Probiotics in food. Health and nutritional properties and guidelines for evaluation. FAO Food and Nutrition Paper, Roma, p 85

  • Fernandez MA, Marette A (2017) Potential health benefits of combining yogurt and fruits based on their probiotic and prebiotic properties. Adv Nutr 8:155S–164S

    Article  Google Scholar 

  • Fernandez-Orozco R, Piskula MK, Zielinski H, Kozlowska H, Frias J, Vidal-Valverde C (2006) Germination as a process to improve the antioxidant capacity of Lupinus angustifolius L. var. Zapaton. Eur Food Res Technol 223:495

    Article  CAS  Google Scholar 

  • Fuller R (2012) Probiotics: the scientific basis. Springer Science & Business Media

  • Garro MS, de Valdez GF, Oliver G, de Giori GS (1998) Growth characteristics and fermentation products of Streptococcus salivarius subsp. thermophilus, Lactobacillus casei and L. fermentum in soymilk. Z Lebensmittelunters Forsch A 206:72–75

    Article  CAS  Google Scholar 

  • Gibson GR, Roberfroid MB (1995) Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr 125:1401–1412

    Article  CAS  Google Scholar 

  • Heydari S, Amiri-Rigi A, Ehsani MR, Mohammadifar MA, Khorshidian N, Koushki MR, Mortazavian AM (2018) Rheological behaviour, sensory properties and syneresis of probiotic yoghurt supplemented with various prebiotics. Int J Dairy Technol 71:175–184

    Article  CAS  Google Scholar 

  • Hong Y-H, Wang S-C, Hsu C, Lin B-F, Kuo Y-H, Huang C-J (2010) Phytoestrogenic compounds in alfalfa sprout (Medicago sativa) beyond coumestrol. J Agric Food Chem 59:131–137

    Article  Google Scholar 

  • Jafari M, Mortazavian AM, Hosseini H, Safaei F, Khaneghah AM, Sant'Ana AS (2017) Probiotic bacillus: fate during sausage processing and storage and influence of different culturing conditions on recovery of their spores. Food Res Int 95:46–51

    Article  CAS  Google Scholar 

  • Jain SK, Jain A, Gupta Y, Ahirwar M (2007) Design and development of hydrogel beads for targeted drug delivery to the colon. AAPS PharmSciTech 8:E34–E41

    Article  Google Scholar 

  • Jayamanne VS, Adams MR (2009) Modelling the effects of pH, storage temperature and redox potential (Eh) on the survival of bifidobacteria in fermented milk. Int J Food Sci Technol 44:1131–1138

    Article  CAS  Google Scholar 

  • Kaur M, Asthir B, Mahajan G (2017) Variation in antioxidants, bioactive compounds and antioxidant capacity in germinated and ungerminated grains of ten rice cultivars. Rice Sci 24:349–359

    Article  Google Scholar 

  • Khalil R, El-Halafawy K, Mahrous H, Kamaly K, Frank J, El Soda M (2007) Evaluation of the probiotic potential of lactic acid bacteria isolated from faeces of breast-fed infants in Egypt. Afr J Biotechnol 6:939–949

    Google Scholar 

  • Khanniri E, Sohrabvandi S, Mortazavian AM, Khorshidian N, Malganji S (2018) Effect of fermentation, cold storage and carbonation on the antioxidant activity of probiotic grape beverage. Curr Nutr Food Sci 14:335–340

    Article  CAS  Google Scholar 

  • Kirtzalidou E, Pramateftaki P, Kotsou M, Kyriacou A (2011) Screening for lactobacilli with probiotic properties in the infant gut microbiota. Anaerobe 17:440–443

    Article  CAS  Google Scholar 

  • Lamsal B, Faubion J (2009) The beneficial use of cereal and cereal components in probiotic foods. Food Rev Int 25:103–114

    Article  CAS  Google Scholar 

  • Lorenz K, D'Appolonia B (2009) Cereal sprouts: composition, nutritive value, food applications. CRC Crit Rev Food Sci Nutr 13:353–385

    Article  Google Scholar 

  • Mohammadi R, Mortazavian A (2011) Technological aspects of prebiotics in probiotic fermented milks. Food Rev Int 27:192–212

    Article  Google Scholar 

  • Mohammadi R, Yousefi M, Sarlak Z, Shah NP, Mortazavian AM, Sadeghi E, Khajavi MZ (2017) Influence of commercial culture composition and cow milk to soy milk ratio on the biochemical, microbiological, and sensory characteristics of a probiotic fermented composite drink. Food Sci Biotechnol 26:749–757

    Article  CAS  Google Scholar 

  • Morah F (2017) Effect of sprouting on nutritional value of Panicium miliaceum (proso millet). Edorium J Nutr Diet 4:1–4

    Google Scholar 

  • Nazzaro F, Fratianni F, Nicolaus B, Poli A, Orlando P (2012) The prebiotic source influences the growth, biochemical features and survival under simulated gastrointestinal conditions of the probiotic Lactobacillus acidophilus. Anaerobe 18:280–285

    Article  CAS  Google Scholar 

  • Nighswonger BD, Brashears MM, Gilliland SE (1996) Viability of Lactobacillus acidophilus and Lactobacillus casei in fermented milk products during refrigerated storage1. J Dairy Sci 79:212–219

    Article  CAS  Google Scholar 

  • Noori N, Hamedi H, Kargozari M, Shotorbani PM (2017) Investigation of potential prebiotic activity of rye sprout extract. Food Biosci 19:121–127

    Article  CAS  Google Scholar 

  • Pan X-D, Chen F-Q, Wu T-X, Tang H-G, Zhao Z-Y (2009) Prebiotic oligosaccharides change the concentrations of short-chain fatty acids and the microbial population of mouse bowel. J Zhejiang Univ Sci B 10:258–263

    Article  CAS  Google Scholar 

  • Rouhi M, Mohammadi R, Mortazavian AM, Sarlak Z (2015) Combined effects of replacement of sucrose with d-tagatose and addition of different probiotic strains on quality characteristics of chocolate milk. Dairy Sci Technol 95:115–133

    Article  CAS  Google Scholar 

  • Sarteshnizi RA, Hosseini H, Khosroshahi NK, Shahraz F, Khaneghah AM, Kamran M, Komeili R, Chiavaro E (2017) Effect of resistant starch and β-glucan combination on oxidative stability, frying performance, microbial count and shelf life of prebiotic sausage during refrigerated storage. Food Technol Biotechnol 55:475

    Article  CAS  Google Scholar 

  • Semyonov D, Ramon O, Kovacs A, Friedlander L, Shimoni E (2012) Air-suspension fluidized-bed microencapsulation of probiotics. Dry Technol 30:1918–1930

    Article  CAS  Google Scholar 

  • Stone H (2012) Sensory evaluation practices. Academic Press

  • Tárrega A, Costell E (2007) Colour and consistency of semi-solid dairy desserts: instrumental and sensory measurements. J Food Eng 78:655–661

    Article  Google Scholar 

  • Tharmaraj N, Shah N (2003) Selective enumeration of Lactobacillus delbrueckii ssp. bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, bifidobacteria, Lactobacillus casei, Lactobacillus rhamnosus, and propionibacteria. J Dairy Sci 86:2288–2296

    Article  CAS  Google Scholar 

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Acknowledgements

This study was part of a Ph.D. thesis Mohsen Mohammadi in Food Science.

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Correspondence to Leila Nouri.

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Mohammadi, M., Nouri, L. & Mortazavian, A.M. Development of a functional synbiotic beverage fortified with different cereal sprouts and prebiotics. J Food Sci Technol 58, 4185–4193 (2021). https://doi.org/10.1007/s13197-020-04887-4

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  • DOI: https://doi.org/10.1007/s13197-020-04887-4

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