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Isolation, characterization and effect of plant-growth-promoting rhizobacteria on pine seedlings (Pinus pseudostrobus Lindl.)

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Abstract

In this study, 10 bacterial strains were isolated from the rhizosphere of coniferous trees on Mount Tláloc in Mexico. The strains were characterized by their capacity to produce auxins, solubilize phosphates and stimulate mycelial growth of the ectomycorrhizal fungus Suillus sp. All isolates were identified at the molecular level. Moreover, an experiment was established to evaluate the response of Pinus pseudostrobus seedlings to inoculation with the rhizobacteria strains. The isolated strains belonged to the species Cupriavidus basilensis, Rhodococcus qingshengii, R. erythropolis, Pseudomonas spp., P. gessardii, Stenotrophomonas rhizophila and Cohnella sp. All of the strains produced auxins; the best producer was R. erythropolis CPT9 (76.4 µg mL−1). P. gessardii CPT6 solubilized phosphate at a significant level (443 µg mL−1). The strain S. rhizophila CPT8 significantly increased the radial growth of the ectomycorrhizal fungus Suillus sp. by 18.8%. Five strains increased the dry mass of the shoots; R. qingshengii CPT4 and R. erythropolis CPT9 increased growth the most, by more than 20%. Inoculation with plant-growth-promoting rhizobacteria can be a very useful practice in a forest nursery to produce healthy, vigorous plants.

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Authors and Affiliations

  1. Área de Microbiología, Postgrado de Edafología, Colegio de Postgraduados, Carretera México-Texcoco km 36.5, CP 56230, Montecillo, Estado de México, Mexico

    Cristina Heredia-Acuña, Juan J. Almaraz-Suarez, Ronald Ferrera-Cerrato & Deisy Y. Pineda-Mendoza

  2. Laboratorio de Recursos Genéticos Microbianos, Centro Nacional de Recursos Genéticos, INIFAP, Boulevard de la Biodiversidad No. 400, CP 47600, Tepatitlán de Morelos, Jalisco, Mexico

    Ramón Arteaga-Garibay

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  1. Cristina Heredia-Acuña
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  2. Juan J. Almaraz-Suarez
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  3. Ramón Arteaga-Garibay
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  4. Ronald Ferrera-Cerrato
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  5. Deisy Y. Pineda-Mendoza
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Correspondence to Juan J. Almaraz-Suarez.

Additional information

Project funding: The work was supported by the project “Impact of Climatic Change and Agricultural Activity on the Emission of Greenhouse Gases and on the Microbial Resources of the Sierra Nevada, Mexico” [“Impacto del Cambio Climáticos y actividad agrícola en la emisión de gases de efecto invernadero y en los recursos microbianos de la Sierra Nevada, México”] No. 213059 funded by the National Council of Science and Technology [Consejo Nacional de Ciencia y Tecnología (CONACyT)].

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Corresponding editor: Tao Xu.

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Heredia-Acuña, C., Almaraz-Suarez, J.J., Arteaga-Garibay, R. et al. Isolation, characterization and effect of plant-growth-promoting rhizobacteria on pine seedlings (Pinus pseudostrobus Lindl.). J. For. Res. 30, 1727–1734 (2019). https://doi.org/10.1007/s11676-018-0723-5

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