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Cloning of four DREB genes from Tibetan Sophora moorcroftiana and analysis of their expression during abiotic stress

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Abstract

Sophora moorcroftiana is an endemic, drought-resistant shrub that grows in Tibet and has some degree of resistance to salt, cold, heat, and drought. In the present study, four dehydration responsive element-binding (DREB) genes (SmDREB1, SmDREB2, SmDREB and SmDREB1) were isolated from S. moorcroftiana for the first time and their expression and proline content under abiotic stress were analyzed. Proline accumulated in seedlings under drought, salt, cold, and heat stress treatments. The four genes were variously expressed in response to the four abiotic stresses. SmDREB1 was induced by drought, cold, and heat stresses; SmDREB2 and SmDREB4 were both induced by salt, cold, and heat stresses, whereas SmDREB3 was induced by drought and heat stresses. Thus, these four genes may participate in conferring tolerance to these four abiotic stresses and are candidate genes for genetic engineering in the future.

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Authors and Affiliations

  1. Agricultural and Animal Husbandry College, Tibet University, Linzhi, 860000, Tibet, People’s Republic of China

    Weijie Yao, Yaru Fu, Yanfu Zhang & Hui-e Li

  2. Key Laboratory of Forest Ecology in Tibet Plateau (Tibet University), Ministry of Education, Linzhi, 860000, Tibet, People’s Republic of China

    Weijie Yao, Yaru Fu & Yanfu Zhang

  3. National Key Station for Field Scientific Observation & Experiment, Linzhi, 860000, Tibet, People’s Republic of China

    Weijie Yao, Yaru Fu & Yanfu Zhang

Authors
  1. Weijie Yao
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  2. Yaru Fu
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  3. Yanfu Zhang
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  4. Hui-e Li
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Correspondence to Hui-e Li.

Additional information

Project funding: This work was supported by the National Natural Science Foundation of China (31260189) and Collaborative Innovation Center Construction of Research and Development on Tibetan Characteristic Agricultural and Animal Husbandry Resources on plateau ecology. Animal Husbandry Resources and Promotion Plan of Plateau Basic Ecological Academic Team Ability, Agricultural and Animal Husbandry College, Tibet University.

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Corresponding editor: Hu Yanbo

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Yao, W., Fu, Y., Zhang, Y. et al. Cloning of four DREB genes from Tibetan Sophora moorcroftiana and analysis of their expression during abiotic stress. J. For. Res. 27, 675–683 (2016). https://doi.org/10.1007/s11676-015-0179-9

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  • DOI: https://doi.org/10.1007/s11676-015-0179-9

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