Abstract
Objective
To evaluate the effect of Buyang Huanwu Decoction (补阳还五汤, BYHWD) on glial scar after intracerebral hemorrhage (ICH) and investigate the underlying mechanism.
Methods
Collagenase type VII (0.5 U) was injected stereotaxically into right globus pallidus to induce ICH model. One hundred and twenty Sprague-Dawley rats were randomly divided into 3 groups according to a random number table, including normal group (n=40), ICH model group (n=40) and BYHWD group (n=40), respectively. After ICH, the rats in the BYHWD group were intragastrically administered with BYHWD (4.36 g/kg) once a day for 21 days, while the rats in ICH group were administered with equal volume of distilled water for 21 days, respectively. Double immunolabeling was performed for proliferating cell nuclear antigen (PCNA)+/glial fibrillary acidic protein (GFAP)+ nuclei. The expression of GFAP and leukemia inhibitory factor (LIF) was evaluated by immunohistochemistry and quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR).
Results
The astrocytes with hypertrophied morphology around the hematoma was observed on day 3 after ICH. The number of GFAP positive cells and GFAP mRNA levels increased notably on day 3 and reached the peak on day 14 post-ICH (P<0.01). PCNA+/GFAP+ nuclei were observed around the hematoma and reached the peak on day 14 post-ICH (P<0.01). In addition, LIF-positive astrocytes and LIF mRNA level in the hemorrhagic region increased significantly till day 14 post-ICH (P<0.01). However, BYHWD not only reduced the number of PCNA+/GFAP+ nuclei, but also decreased GFAP and LIF levels (P<0.05).
Conclusions
BYHWD could attenuate ICH-induced glial scar by downregulating the expression of LIF in the rats.
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Supported by the National Natural Science Foundation of China (No. 81202625, 30400581, 30873221 and 81173175), the Project for New Century Excellent Talents (NCET-11-0522), the Hunan Provincial Natural Science Foundation (No. 07JJ5007 and 10JJ2023), the Hubei Provincial Natural Science Foundation (No. 2017CFB468) and the Key Laboratory of Cardiovascular and Cerebrovascular Diseases Translational Medicine (Three Gorges University, No. 2016KXN06)
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Kang, X., Zhou, Hj., Yang, J. et al. Buyang Huanwu Decoction (补阳还五汤) Attenuates Glial Scar by Downregulating the Expression of Leukemia Inhibitory Factor in Intracerebral Hemorrhagic Rats. Chin. J. Integr. Med. 25, 264–269 (2019). https://doi.org/10.1007/s11655-018-2917-7
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DOI: https://doi.org/10.1007/s11655-018-2917-7