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Plantlet regeneration via somatic embryogenesis and changes in endogenous hormone content of Rosa ‘John F. Kennedy’

  • Plant Tissue Culture
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Abstract

This study describes a plantlet regeneration protocol of somatic embryos in Rosa ‘John F. Kennedy’ (hybrid tea rose). Different somatic embryo sizes exhibited significant differences in the single bud (SB type) regeneration rate and multiple bud (MB type) regeneration rate. The highest single bud (SB type) regeneration rate (27.10%) was obtained from the large size (4 mm × 5 mm). The multiple bud regeneration rate was highest at 39.60% for the medium size (3 mm × 4 mm). Changes in the endogenous hormone content and ratios of various types of embryogenic cultures were clearly diverse: higher contents of abscisic acid (ABA) and indole-3-acetic acid (IAA) occurred in the SPC explant (single-piece cotyledonary somatic embryo) with a regenerated single bud (SB type). In a MW-type somatic embryo (milky-white single-piece-cotyledon explant), the gibberellic acid (GA3)/ABA ratio was the highest (1.807), and the IAA/GA3 ratio was the lowest (0.902). However, the highest ratios of IAA/GA3 (6.159) and the lowest ratios of GA3/ABA (0.383) appeared in SB-type cultures. Additionally, the highest IAA/ABA ratios (6.535) and higher ratios of GA3/ABA (1.729) were found in MB-type cultures. This indicated that ways to regulate plant cell totipotency in Rosa ‘John F. Kennedy’ somatic embryos differed between single bud (SB type) regeneration and multiple bud (MB type) regeneration. Finally, this study classified and summarized common intermediate materials in in vitro culture based on morphological characteristics and plantlet regeneration pathways.

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Acknowledgements

The authors thank Ms. MYZ (Nanyang Academy of Agricultural Science) who provided plant material for this research.

Funding

This work was supported by the Postgraduate Education Reform and Quality Improvement Project of Henan Province, Grant No. YJS2021JD17; the Natural Science Foundation of Henan Province of China, Grant No. 232300420450; and the Key Research Project of Colleges and Universities of Henan Province Education Department, Grant No. 24B210008.

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Authors and Affiliations

  1. Analysis and Test Center, Nanyang Normal University, Nanyang, 473061, China

    Li Du

  2. Henan Province Engineering Research Center of Rose Germplasm Innovation and Cultivation Technique, Nanyang Normal University, Nanyang, 473061, China

    Li Du, Xiaoling Kang, Zhongfeng Zhu, Yu Ma, Haoran Guo, Jingman Li & Chuanyu Ding

  3. College of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang, 473061, China

    Xiaoling Kang, Zhongfeng Zhu, Yu Ma, Haoran Guo, Jingman Li & Chuanyu Ding

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  1. Li Du
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  4. Yu Ma
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  7. Chuanyu Ding
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Contributions

All authors contributed to the study conception and design. LD guided the experiment, data analysis, writing the manuscript, and final editing. CYD participated in the investigation, methodology, and validation. XLK and ZFZ carried out all the experiment work and collected the data. YM, HRG, and JML participated in the writing of the introduction, data analysis, software operation, and survey validation. All authors commented on and contributed to the writing and revision of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Chuanyu Ding.

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Du, L., Kang, X., Zhu, Z. et al. Plantlet regeneration via somatic embryogenesis and changes in endogenous hormone content of Rosa ‘John F. Kennedy’. In Vitro Cell.Dev.Biol.-Plant (2024). https://doi.org/10.1007/s11627-024-10426-z

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