Abstract
In this study, a new method for shoot regeneration via protocorm-like bodies (PLBs) is described. Shoot proliferation using stem elongation under light-emitting diodes (LEDs), and enhanced growth of Anthurium andraeanum ‘Tropical’ plantlets on medium containing silver nanoparticles (AgNPs) was investigated. For shoot regeneration, the micropropagation efficiency via PLB formation was fourfold more efficient than directly from callus after 12 wk of culture. This was possible because each initial callus cluster could be cut into 20 explant slices (1 × 5 × 5 mm in size), each with an average of 42.67 PLBs which are capable of growing into plants. For shoot proliferation, single shoots with 2 cm in size derived from PLB culture were placed under different blue to red LED ratios. This study showed that higher shoot height (4.77 cm), fresh weight (327.33 mg), number of leaves per shoot (6.33), and the number of stem nodes per shoot (5.67 stem nodes) were obtained under 70R:30B as compared to those under other lighting conditions and fluorescent lamp after 8 wk of culture. Especially, the total shoot proliferation (10 shoots) via stem node culture was higher than those in the control. For enhanced plantlet growth, single shoot with a pair of leaves derived from stem elongation under 70R:30B cultured on 1/4 MS medium supplemented with 0.5 mg L−1 NAA and 1 g L−1 activated charcoal, 7 mg L−1 AgNPs, 30 g L−1 sucrose, and 8 g L−1 agar gave the highest growth than other treatments. Plantlets derived from 7 mg L−1 AgNP treatment transferred into greenhouse showed better growth as well as 4-wk earlier flowering than the control treatment.
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Acknowledgements
The authors would like to thank Professors Edward CT Yeung and C.C. Chinnappa (University of Calgary, Canada) for critical reading of the manuscript.
Funding
This research is funded by Vietnam National Foundation for Science and Technology Development (NAFOSTED) under grant number 106.01–2019.301 and Vietnam Academy of Science and Technology under grant number NVCC22.01/21–21 (No. 2510/QĐ-VHL).
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HTT acquired data and wrote the manuscript. PTS, HDK, VQL, DMC, VTH, NBN, HTMN, LTB, and THP participated in performing the experiments, interpretation of data, and revision for intellectual content. DTN and HTT conceptualized and designed the study. All authors discussed the results and contributed to the final manuscript.
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Tung, H.T., Suong, P.T., Khai, H.D. et al. Protocorm-like body formation, stem elongation, and enhanced growth of Anthurium andraeanum ‘Tropical’ plantlet on medium containing silver nanoparticles. In Vitro Cell.Dev.Biol.-Plant 58, 70–79 (2022). https://doi.org/10.1007/s11627-021-10217-w
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DOI: https://doi.org/10.1007/s11627-021-10217-w