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Silver Nanoparticle-Mediated Enhancement in Growth and Antioxidant Status of Brassica juncea

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Abstract

Metal nanoparticles can potentially be used as tools for engineering biological redox reactions. Present study underlines the effect of silver metal nanoparticles (at 0, 25, 50, 100, 200 and 400 ppm) on the growth and antioxidant status of 7-day-old Brassica juncea seedlings. Fresh weight, root and shoot length, and vigor index of seedlings is positively affected by silver nanoparticle treatment. It induced a 326 % increase in root length and 133 % increase in vigor index of the treated seedlings. Improved photosynthetic quantum efficiency and higher chlorophyll contents were recorded in leaves of treated seedlings, as compared to the control seedlings. Levels of malondialdehyde and hydrogen peroxide decreased in the treated seedlings. Nanoparticle treatment induced the activities of specific antioxidant enzymes, resulting in reduced reactive oxygen species levels. Decrease in proline content confirmed the improvement in antioxidant status of the treated seedlings. The observed stimulatory affects of silver nanoparticles are found to be dose dependent, with 50 ppm treatment being optimum for eliciting growth response. Present findings, for the first time indicate that silver nanoparticles promote the growth of B. juncea seedlings by modulating their antioxidant status.

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Acknowledgments

The authors are thankful to the Department of Biotechnology, Govt. of India, for financial support. We thank, the Head, Department of Genetics, Indian Agricultural Research Institute, New Delhi for providing seed material.

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Authors and Affiliations

  1. Department of Molecular Biology & Genetic Engineering, G B Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, India

    Priyadarshini Sharma, Deepesh Bhatt & Sandeep Arora

  2. Department of Chemistry, G B Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, India

    M. G. H. Zaidi

  3. Department of Environmental Studies, University of Delhi, Delhi, India

    P. Pardha Saradhi

  4. Defense Institute of Advance Technology, Pune, India

    P. K. Khanna

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  1. Priyadarshini Sharma
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  2. Deepesh Bhatt
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  3. M. G. H. Zaidi
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  4. P. Pardha Saradhi
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  5. P. K. Khanna
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  6. Sandeep Arora
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Correspondence to Sandeep Arora.

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Sharma, P., Bhatt, D., Zaidi, M.G.H. et al. Silver Nanoparticle-Mediated Enhancement in Growth and Antioxidant Status of Brassica juncea . Appl Biochem Biotechnol 167, 2225–2233 (2012). https://doi.org/10.1007/s12010-012-9759-8

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