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Enhanced somatic embryogenesis and plantlet regeneration in Cenchrus ciliaris L.

  • Plant Tissue Culture
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Abstract

A highly reproducible plant regeneration protocol through somatic embryogenesis and shoot organogenesis has been developed for Cenchrus ciliaris. Three explants (seeds, shoot apices, and immature inflorescences) of four genotypes (IG-3108, IG-718, IG-74, and DBC15-8/32/10) were used for callus induction and plant regeneration. The highest rate of callus formation was found using Murashige and Skoog (MS) medium supplemented with 0.5 mg L−1 benzylaminopurine (BA) and 3.0 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D). The largest number of somatic embryos was generated with the addition of 400 mg L−1 L-proline, 400 mg L−1 L-glutamine, and 300 mg L−1 casein hydrolysate. Somatic embryos were successfully germinated on MS medium with 3.0 mg L−1 BA and 0.25 mg L−1 2,4-D. In vitro plant regeneration was accomplished through somatic embryogenesis using all three explants. Ultra-structural features of somatic embryos confirmed proper formation and ontogeny.

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Funding

This research was supported by the R & D grant, University of Delhi. This paper is presented by Shashi in partial fulfillment of requisites for a thesis of Doctor of Philosophy in Botany at the University of Delhi, India.

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  1. Plant Developmental Biology, Department of Botany, University of Delhi, Delhi, 110 007, India

    Shashi & Vishnu Bhat

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  1. Shashi
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  2. Vishnu Bhat
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Correspondence to Vishnu Bhat.

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Editor: Charles Armstrong

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Shashi, Bhat, V. Enhanced somatic embryogenesis and plantlet regeneration in Cenchrus ciliaris L.. In Vitro Cell.Dev.Biol.-Plant 57, 499–509 (2021). https://doi.org/10.1007/s11627-020-10148-y

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  • DOI: https://doi.org/10.1007/s11627-020-10148-y

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