Summary
Histological information is presented on the origin of initial tissue proliferation and on embryogenesis and organogenesis in sub-cultured tissue derived from mature orchardgrass (Dactylis glomerata L.) embryos. Embryos were plated on an LS agar medium containing 20 μM 2,4-D. Examination of cultures between 96 and 144 hours after plating showed parenchyma proliferation originating primarily from the coleorhiza, especially the basal portion. Within 28 days after plating, the tissue showed various degrees and kinds of organized structures including lobed meristematic regions with vascular tissue. These are thought to develop ultimately into aerial roots which are common in grass tissue cultures. Subcultured tissue on 1.0 μM 2,4-D medium showed somatic embryos completely isolated from the tissue mass suggestingde novo embryogenesis. Organogenesis was evident by shoot apical meristems existing on the surface of the tissue mass without attached roots and root meristems without shoots within the tissue. The observations are discussed in relation to the anatomy of the grass embryo.
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This research was supported in part by the Competitive Research Grants Office of the U.S. Department of Agriculture under Agreement No. 5901-0410-9-0331-0.
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McDaniel, J.K., Conger, B.V. & Graham, E.T. A histological study of tissue proliferation, embryogenesis, and organogenesis from tissue cultures ofDactylis glomerata L.. Protoplasma 110, 121–128 (1982). https://doi.org/10.1007/BF01281538
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DOI: https://doi.org/10.1007/BF01281538