Abstract
In vitro investigation of bovine lactation processes is limited by a lack of physiologically representative cell models. This deficiency is most evident through the minimal or absent expression of lactation-specific genes in cultured bovine mammary tissues. Primary bovine mammary epithelial cells (pbMECs) extracted from lactating mammary tissue and grown in culture initially express milk protein transcripts at relatively representative levels. However, expression drops dramatically after only three or four passages, which greatly reduces the utility of primary cells to model and further examine lactogenesis. To investigate the effects of alternate alleles in pbMECs including effects on transcription, we have developed methods to deliver CRISPR-Cas9 gene editing reagents to primary mammary cells, resulting in very high editing efficiencies. We have also found that culturing the cells on an imitation basement membrane composed of Matrigel, results in the restoration of a more representative lactogenic gene expression profile and the cells forming three-dimensional structures in vitro. Here, we present data from four pbMEC lines recovered from pregnant cows and detail the expression profile of five key milk synthesis genes in these MECs grown on Matrigel. Additionally, we describe an optimised method for preferentially selecting CRISPR-Cas9-edited cells conferring a knock-out of DGAT1, using fluorescence-activated cell sorting (FACS). The combination of these techniques facilitates the use of pbMECs as a model to investigate the effects of gene introgressions and genetic variation in lactating mammary tissue.
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Introduction
Physiologically relevant modelling of biological processes in cultured cells, particularly those isolated from terminally differentiated tissues, is challenging. Cells obtained from biopsies lose their specific gene expression profiles after a small number of divisions in vitro (Jedrzejczak and Szatkowska 2014). Changes in cell type representation can further complicate the culture of somatic cells for functional modelling. For example, some cell types, such as fibroblasts, can become the dominant cell population because of their relatively rapid division in a mixed cell culture (Pal and Grover 1983). The immortalisation of cells isolated from somatic tissue facilitates an infinite window in which cells can be cultured but often leads to compromised characteristics not representative of the primary tissue (Zhou et al. 2008). A potential solution to this problem is to apply a new methodology to enable the extended use of primary cells while they remain in their pre-immortalised state.
There has been considerable effort to uncover genetic and biochemical influences on bovine lactational traits using in vitro cell models of mammary tissue (Kumura et al. 2001; Sakamoto et al. 2005; Hernandez et al. 2008). The most frequently used models are immortalised cell lines, such as MAC-T (Huynh et al. 1991) or BME-UV (Zavizion et al. 1996), which maintain the ability to proliferate. However, after multiple generations in culture, these cells lack many important, physiologically relevant lactogenic characteristics such as milk protein expression. In contrast, primary bovine lactating mammary epithelial cells (pbMECs) in culture initially have high milk protein gene expression levels. Unfortunately, this phenotype changes significantly over a few passages, where expression of milk protein genes often reduces and becomes undetectable (Jedrzejczak and Szatkowska 2014). During the culturing of pbMECs, lactogenic hormones such as dexamethasone and prolactin are added to culture media to induce or help maintain some level of the lactogenic phenotype (Kumura et al. 2001; Sakamoto et al. 2005; Zhao et al. 2010).
Furthermore, the growth of pbMECs on an artificial basement membrane and the formation of three-dimensional (3D) structures has been shown to support the differentiation of pbMECs into ductal and luminal epithelial cells (Rose et al. 2002; Boutinaud et al. 2015). This formation of 3D structures is associated with a more sustained profile of alpha, beta, and kappa casein gene expression compared to cells grown in 2D (Hillreiner et al. 2017). Matrigel is a complex solution containing many extracellular matrix proteins secreted from mouse sarcoma cells (Kibbey 1994). When attached to plastic culture dishes, it mimics the basement membrane and facilitates the attachment of cultured cells. The structural support of the Matrigel is thought to trigger differentiation and return epithelial cells to a more lactogenic state compared to monolayer growth on plastic (Mroue and Bissell 2013).
The advent of efficient Clustered Regularly Interspaced Short Palindromic Repeat – Cas9 (CRISPR-Cas9) targeted editing has enabled the dissection of phenotypic consequences due to genetic changes at specific sites (Liu et al. 2019b). The CRISPR-Cas9 system is a multifunctional tool that is now frequently used to edit genomes, allowing the researcher to assess the effects of the induced genetic changes on gene function or expression in many model systems (Ebrahimi and Hashemi 2020). To date, there have only been a few reports describing the use of gene editing to investigate the effects of genetic variants on lactogenic gene expression in lactating mammary epithelial cells (Tian et al. 2018; Huang et al. 2020; Edick et al. 2021). A major limitation of this research is that the number of cell divisions required to complete experiments is often outside the window of physiologically representative gene expression in pbMECs. A number of factors influence the editing efficiency of the CRISPR-Cas9 complex; the activity of the guide RNA, the number of complexes a cell is transfected by, and the availability of the genomic region being edited. This intrinsic variation is particularly concerning when a heterogeneous population of cells rather than a clonal population is to be analysed (Fu et al. 2021).
Here, we present a combined protocol, which describes the culture and characterisation of pbMECs and their transfection and editing, using the critical gene for lactogenesis, DGAT1, as an example of this methodology. We demonstrate how fluorescence-activated cell sorting (FACS) can greatly improve the frequency of edited cells in the final cell population. We also show that growth of the sorted CRISPR-Cas9-edited pbMCEs on Matrigel improves the maintenance of lactogenic gene expression. The combination of these methodologies opens the window for the testing of gene variant function in primary cell lines.
Materials and Methods
Establishing an epithelial cell line
pbMECs were derived from parenchymal mammary tissue obtained after slaughter, from four cows at 6 to 7 mo of their first pregnancy. The animals were obtained from the Ruakura Dairy Farm and were slaughtered in accordance with MPI regulations at the AgResearch abattoir on the Ruakura Farm, under the AgResearch ethics approval number 3925. The Ruakura Animal Ethics Committee approved the study. These methods are in accordance with the ARRIVE guidelines. The tissue was washed in Hanks’ Balanced Salt Solution (10 ×) (HBSS) and then digested using Digest Medium (Table 1.) for 2 h at 37 °C. The tissue was then minced using sterile curved scissors and scalpel blades and put back into Digestion Medium for 3 h to remove all other connective tissue. Cells were filtered through a 150 μm mesh filter and washed three times in Wash Medium (Table 1.) at 100 × g for 10 min, after which cells were resuspended in 5 mL of HBSS. Finally, cells were fractionated by centrifugation at 800 g for 20 min using a Percoll gradient with six fractions. Epithelial cells from the 1.03–1.05 (g/mL) fraction were removed and washed in HBSS (10 ×). These cell harvest and purification steps were performed in 2001, and the resultant cells were subsequently frozen in 20% DMSO and stored in liquid nitrogen. Revival of cells was conducted by suspension of the vial in a 37 °C water bath until no ice remained. Thawed cells were added to proliferation media (10% foetal bovine serum (FBS), 1% penicillin/streptomycin, 5 µg/mL insulin, 1 µg/mL progesterone, Dulbecco’s Modified Eagle Medium (DMEM)) and pelleted at 500 × g for 5 min. Cells were then added to differentiation media (DM) containing 10% FBS, 1% penicillin/streptomycin, 5 µg/mL insulin, 10 µg/mL dexamethasone, and 5 µg/mL prolactin in DMEM. DM is used to stimulate the cells to differentiate into a more lactogenic state. The lines used for subsequent experiments are referred to in the manuscript as the Pink, Orange, Green, and Brown lines.
Immunocytochemistry
All cell lines were seeded at 300,000 cells per well, and grown to 95% confluency for five passages in a 6-well plate before being fixed in the well with ice cold, 100% methanol. Cells were incubated with 1% BSA, 22.52 mg/mL glycine in PBST (PBS + 0.1% Tween 20) to block non-specific antibody binding before being incubated for 1 h at room temperature in a 1:1000 dilution of the Anti-Cytokeratin 8 primary antibody [C-43] (FITC) (ab176533). Following a PBS wash, the cells were incubated with the secondary antibody, Alexa Fluor 594 goat anti-mouse IgG (H + L) A11032, at a final concentration of 2 μg/mL in 1% BSA for 1 h at room temperature in the dark. Cells were then counterstained with DAPI at a final concentration of 1 μg/mL before visualisation using a Nikon Ti-E inverted microscope and TxRed (542–644 nm) and DAPI (352–477 nm) fluorescence filter cube.
qPCR of key milk-related gene expression
The expression of five key milk genes was assessed over three consecutive passages (P1-P3) following the thawing of stored pbMEC aliquots. Cells were grown until 90% confluent in one well of a 12-well plate before digestion with Trypsin–EDTA (0.25%) (Gibco - Carlsbad, CA, USA). RNA was extracted from half of the harvested cells from each well using an RNeasy kit (Qiagen - Hilden, Germany) while re-seeding the remaining half into one well of a new 12-well plate. cDNA was generated from RNA using SuperScript™ III First-Strand Synthesis SuperMix (Invitrogen), and subsequently, gDNA contamination was removed using the DNA-free™ Kit (Life Technologies). qPCR assays for relative RNA quantification were undertaken using the Roche Universal Probe Library system on a LightCycler 480 machine for five target genes GPAT4 (formerly AGPAT6), CSN2, DGAT1, PAEP (formerly LGB), and MGST1 and two reference genes EIF3K and RPS15A (Walker et al. 2009). Details of the qPCR primers, probes, and expected amplicon sizes can be found in Table 2.. Delta-Delta-Ct analysis was performed on the raw Ct results using the average results from passage one in each line as the control. A one-tail two-sample equal variance t-test was used to calculate the p-values for the difference in expression between the three passages in each cell line. These data are present in the supplementary materials (Supplementary Table 1). All raw data and calculations can be found at https://doi.org/10.7910/DVN/YATECZ.
Estimating the transfection and editing efficiency of pbMECs
To assess their transfection efficiency, pbMECs at passage 6 were removed from the plastic well using Trypsin–EDTA (0.25%) and seeded in differentiation media into one well of a 24-well plate at 100,000 cells per well in 443 μL of media. Immediately after plating, while the cells were still suspended in the media, they were reverse-transfected, utilising lipofection to deliver the tracrRNA. The reactions consisted of 0.2 μM of ATTO-labelled tracrRNA (IDT, Cat:1,075,927) 2.5 μL of Cas9 reagent, 1.5 μL of CRISPRMAX (Invitrogen), and 50 μL of Opti-MEM. Following the addition of the transfection reagents, the cells were grown for 24 h before visualising the ATTO-labelled tracrRNA using the Nikon Ti-E inverted microscope.
To investigate gene editing of pbMECs using the CRISPR/Cas9 system, the CRISPRMAX reagents were used to reverse transfect a ribonucleoprotein (RNP) complex comprised of the S. pyogenes Cas9 nuclease complexed with one of two different guide RNAs. These guides were designed to cut in two locations and potentially delete a region of DGAT1 exon 1, thereby knocking out expression. The guides were designed using the web-based software CRISPOR, which finds potential guide RNA sequences and ranks them based on their predicted activity and potential off-target effects (Concordet and Haeussler 2018). The crRNA sequences were as follows: guide 1—/AltR1/rGrCrUrArCrGrArCrUrUrGrGrCrCrGrCrGrGrCrGrGrUrUrUrUrArGrArGrCrUrArUrGrC rU/AlTR2/, guide 2—/AltR1/rArArGrGrArCrGrGrArGrArCrGrUrArGrArCrGrUrGrUrUrUrUrArGrArG rCrUrArUrGrCrU/AltR2/, where AltR1 and AltR2 are end-blocking modifiers. The crRNAs were synthesised by IDT. Again, the Invitrogen CRISPRMAX protocol was used to make up the appropriate transfection reactions for a 24-well plate (see above). The RNP complex was assembled at a molar ratio of 1:1.6 guide RNA:Cas9 Protein (IDT, Cat:1,081,058) with a final concentration of 200 nM for each reaction, in a final volume of 500 μL. Cells were seeded in a 24-well plate at 100,000 cells per well before reverse transfection with the RNP complex. After the addition of the editing reagents, the cells were grown for 48 h before they were removed by digestion with 2 mL of Trypsin and DNA extracted using a DNeasy column kit (Qiagen). PCR was undertaken with primers (P1 & P2) flanking the Cas9-mediated cleavage sites (Primer P1: 5′ CAGTTGGCCAAGGGTCCG 3′ and Primer P2 5′ AGGGGTCAAAGGTTAGGGGT 3′). The PCR cycling conditions were as follows: initial denaturation at 95 °C for 3 min, denaturation at 95 °C for 15 s, annealing at 56 °C for 15 s and extension at 72 °C for 15 s for 35 cycles, and a final extension at 72 °C for 1 min. The wild-type product was expected to be 322 bp in length, and the double cut product was expected to be 138 bp.
Culturing pbMECs in 3D
The imitation basement membrane used to coat the plates, Matrigel (Corning, NY), was prepared by 2 × dilution with serum-free DMEM media (Xu and Buchsbaum 2012). Twenty-four-well plate wells were coated with 200 μL of diluted Matrigel by spreading onto the bottom of the wells and incubating at 37 °C for 30 min to solidify. The initial qPCR experiment revealed that the Green pbMEC line had consistent expression of all target genes (Fig. 4). Therefore, the Green pbMEC line was selected for 3D culturing experiments.
Cells stored in liquid nitrogen were thawed and plated on plastic in differentiation media to recover for 2 d (Fig. 1). After 2 d of growth on plastic, 2 × 105 of these Passage 1 cells were plated onto Matrigel, grown for 8 d, removed from the Matrigel using 600 μL of cell recovery solution (Corning) to keep the 3D structures intact, and re-seeded onto Matrigel. These cells were grown for a further 8 d before extraction from the wells using the cell recovery solution. In parallel, 3 × 105 passage 1 cells were re-seeded back onto a plastic 6-well plate where they were grown for 5 d before following the protocol as above, with two more passages completed on Matrigel. Each passage from plastic to plastic, or plastic to Matrigel is denoted as P1, P2, or P3. Passaging from Matrigel to Matrigel is denoted as P2.1 and P3.1 (see Fig. 1 for details). A one-tail two-sample equal variance t-test was used to calculate the p-values for the difference in expression seen between passage 2 cells grown on plastic or Matrigel in Fig. 7.
DGAT1 knock-out via CRISPR/Cas9 editing
An initial qPCR experiment measuring the relative expression of milk production genes in the 4 primary lines revealed that the Brown pbMEC line had the highest expression of CSN2 and LGB (relative expression was 4.84 and 2.9, respectively) after the first passage, suggesting the retention of a physiologically relevant phenotype. An aliquot of these cells was thawed and passaged once and then 3 wells of a 6-well plate were seeded into differentiation media, with 1 × 106 cells in each well (Fig. 2). Wells A1 and A2 of the 6-well plate were reverse transfected by CRISPRMAX (12.5 μL of Cas9 reagent, 7.5 μL of CRISPRMAX (Invitrogen), and 250 μL of Opti-MEM). The transfection introduced an RNP complex containing DGAT1 knock-out dual guide RNAs, at a final concentration of 200 nM for both guides combined, in a total volume of 2 mL. The liposomes in the A1 also contained ATTO-labelled tracrRNA at a final concentration of 200 nM, which was co-transfected in addition to the RNP complex. The second well (A2) was only transfected with the RNP complex, and the third well (A3) of cells was a non-transfected control. After 24 h in culture, the cells were detached using Trypsin–EDTA (0.25%) and 1 × 104 cells from wells A2 and A3 were each plated in three wells of a 96-well plate. The ATTO-transfected cells (well A1) were FACS sorted on a BD FACS Aria II SORP (BD) after using DAPI counterstaining for live cell selection, and using 550 nm ATTO positive signal to sort them into positive and negative pools. These cells were plated into three 96-well plate wells at 1 × 104 cells per well and grown to confluency. Once confluent, the cells from each treatment (3 wells) were pooled for each condition, transferred into a 12-well plate, and grown to confluency. Once 95% confluent, cells from the 12-well plate (edited and sorted ATTO positive cells, edited unsorted cells and control cells) were detached, and 1 × 105 cells per well were seeded into a 24-well plate, into wells that were either plain plastic (two wells) or were coated with Matrigel (three wells). The remaining cells were used for DNA extraction using a DNeasy column kit (Qiagen). PCR was undertaken with different primers (P1_Long & P2_Long) flanking the Cas9-mediated cleavage sites (Primer P1_Long: 5′ CAGCGGACTACAAAGGTATG 3′ and Primer P2_Long 5′ CAACCTCCCGCTAAGTTTC 3′). The wild-type product was expected to be 618 bp in length, and the double cut product was expected to be 434 bp. The KAPA Taq PCR Kit (Merck, Cat: KK1008) was used to complete PCR. These amplicons were sequenced via MinION sequencing (Oxford Nanopore Technologies) which was performed per the MinION 1D Native barcoding genomic DNA protocol, using Ligation sequencing kit (SQK-LSK109) and Barcoding expansion kit (EXP-NBD103).
The cells plated on plastic were cultured for 5 d, and the cells cultured on Matrigel were grown for 2 wk before harvesting using 2 mL of trypsin for the plastic wells and 600 μL of Corning Cell Recovery Solution for the Matrigel wells. RNA was extracted using a Qiagen RNeasy Kit. Cells from the two or three wells for each condition were pooled to ensure sufficient RNA was extracted; that is, wells were not treated as biological replicates. qPCR of the target DGAT1 and the two reference genes EIF3K and RPS15A was then conducted on all samples to determine the relative expression of DGAT in the ATTO positive, RNP only and wild-type cell populations. qPCR was also completed in a preliminary experiment with these methods, and relative expression of GPAT4 and MGST1 genes was assessed. Delta-Delta-Ct analysis was performed on the raw Ct results using the 125 × dilution from the standard curve as the control.
Analysis of potential off target editing sites
The cutting frequency determination (CFD) score of the CRISPOR software was used to identify the top four ranked off-target regions for each guide (Liu et al. 2019a). PCR primer sets designed for these off-target regions can be found in Supplementary Table 2. The iProof™ High-Fidelity PCR Kit (Bio-Rad, Cat: 1,725,330) was used to complete PCR on the ATTO plus RNP (well A1) DNA sample from the DGAT1 knock-out via CRISPR/Cas9 editing experiment. The PCR cycling conditions were as follows: initial denaturation at 98 °C for 30 s, denaturation at 98 °C for 5 s, annealing at 59 °C for 10 s and extension at 72 °C for 15 s for 32 cycles, and a final extension at 72 °C for 5 min. The amplicons were cleaned using a PCR clean up kit (Machery-Nagel, Cat: MN740609.250) and sequenced via Sanger sequencing.
Equipment and settings
All agarose gels were imaged on a Bio-Rad Gel Doc XR + using Image Lab™ software (version 5.2.1). The ‘GelRed’ application was used, and the exposure time was optimised for faint bands. The microscopy images were taken on a Nikon Ti-E inverted microscope using NIS-Elements software (Nikon Instruments Inc). TxRed (542–644 nm) and DAPI (352–477 nm) fluorescence filter cubes were used to visualise the Alexa Fluor 594 goat anti-mouse IgG (H + L) A11032 and DAPI stain. Photoshop was used to enhance the brightness and contrast of the red and blue fluorescence in Fig. 3A and C. This was applied evenly across the entire image.
Results
Epithelial characterisation
After thawing the cryopreserved pbMECs, the cells from all investigated lines were aggregated in the culture media. It took 2 d for these clumps to settle on the bottom of the well and for the epithelial cells to migrate out and form a monolayer. Once confluent, the cells were passaged using trypsin to detach them from the well surface; this also served to disaggregate the clusters further, resulting in a more dispersed pool of cells in the following passage. The pbMECs were grown to passage 5 in one well of a 6-well plate, by which time they grew exclusively as a monolayer and were fixed with methanol for immunocytochemistry (ICC). Cells were stained with a mouse anti-cytokeratin 8 primary antibody and a goat anti-mouse IgG secondary antibody conjugated to a red-fluorescent dye Alexa Fluor 594. Cytokeratin 8 is a cytoskeletal protein shown to identify mammary epithelial cells (Boutinaud et al. 2015). The majority of cells exhibited red staining in the cytoplasm, indicating that the cells were mainly mammary epithelial cells as expected (Fig. 3A, B, and C). To assess the relative gene expression levels of key lactation genes across time in the pbMECs, qPCR was conducted on five target genes. These were triglyceride synthesis genes GPAT4 and DGAT1; CSN2 and PAEP, which are components of milk casein and whey protein, respectively; and MGST1, which has been shown to regulate milk production phenotypes (Littlejohn et al. 2016). RNA was extracted from the four pbMEC cell lines grown on plastic at passages 1, 2, and 3 and qPCR was conducted on these samples. All genes were expressed during at least one of the passages, with expression levels in passage 1 generally the highest (Fig. 4). The expression levels of all the target genes gradually decreased in subsequent passages. Of particular note, CSN2 and PAEP expression was at very low levels after P1. This observation aligns with previously characterised epithelial lines, reporting reduced milk-related gene expression after a few passages. The statistical significance of the differences in the relative expression between passages is summarised in Supplementary Table 1 of the supplementary materials.
Transfection and CRISPR-Cas9 editing of pbMECs
To assess the CRISPR-Cas9 editing capability of the pbMECS, we trialled and optimised several transfection methods. First, we tested the efficiency of the transfection reagent CRISPRMAX to transfect cells with ATTO-labelled tracrRNA. Cells were visualised 24 h post CRISPRMAX-mediated transfection of ATTO-labelled tracrRNA. All lines had a high level of transfection, demonstrated by the presence of ATTO dye within individual cells in the Brown line (Fig. 5A). The cells within the outlined area of Fig. 5A were counted, and the number of red cells were counted in the same region in the TRITC-filtered image. This gave an estimated transfection efficiency of 41.89%, with 31 of 74 cells appearing to contain ATTO dye. Given the successful transfection of pbMECs with ATTO-labelled tracrRNA using the CRISPRMAX lipid, it was decided to proceed with direct gene editing. A dual guide simultaneous cut strategy targeting DGAT1 exon 1 was used with the aim of introducing an exonic deletion. Ribonuclear protein (RNP) complexes were formed with two gRNAs, one targeting cleavage before the Methionine start codon and another targeting a region downstream near the 3′ boundary of exon 1 (Fig. 8A). The four pbMEC lines were transfected with the RNP complexes and cells were cultured for a further 48 h. Cells were then harvested, genomic DNA extracted, and a PCR with primers flanking the targeted region was undertaken. Each pool of cells demonstrated a low but equal level of editing in all cell lines, apparent in the relative intensity of the intact and deletion amplicons (Fig. 5B).
pbMEC growth on Matrigel
Previous studies have shown that established MEC lines maintain lactogenic gene expression when grown on a Matrigel matrix (Prpar Mihevc et al. 2014). The Brown line, cultured in differentiation media, was passaged twice on plastic before being plated onto Matrigel. Cells grown on Matrigel had begun to form 3D structures after 4 d of growth, and after 14 d of culturing, duct-like protrusions connected several mammosphere-like structures in the wells (Fig. 6).
To assess the relative expression over time of the five genes of interest by qPCR, RNA was harvested from freshly thawed Green pbMECs and cells at passages 1 and 2, grown on plastic and Matrigel (Fig. 1). The gene expression results from the freshly thawed, uncultured biopsy cells were used as the control to which the other passages’ gene expression was normalised. As the control always has a relative expression of 1, this result is not plotted on the graphs. The expression levels of the selected genes at passages 1 and 2 cultured on plastic are concordant with the results presented in Fig. 4. Here we found that cellular expression of CSN2 and PAEP decreased across successive passages when the cells were cultured on plastic, while for GPAT4, DGAT1, and MGST1, expression remained stable (Fig. 7). The relative expression levels of all the selected genes for cells grown on Matrigel at passage 2 were higher than cells grown on plastic at passage 1 or 2 (Fig. 7). This difference in gene expression between culturing conditions was significant for DGAT1, MGST1, and CSN2. With the exception of PAEP expression, passaging of the 3D structures between wells containing Matrigel (P2.1 and P3.1) either resulted in increased or stable transcript levels compared to the previous passage. Relative expression of GPAT4, DGAT1, and MGST1 for cells grown and passaged on Matrigel was higher than for P1 cells grown on plastic. Relative β-casein expression increased at passages 2 and 3 with cells cultured on Matrigel relative to plastic in subsequent passages, but the expression was not restored to the level seen at P1.
Knock-out of DGAT1 in pbMECs results in a decrease in gene expression and a change in phenotype
CRISPR-Cas9 editing efficiency of pbMECs using a dual guide RNA approach was relatively low, as determined in a semi-quantitative way by the visualised intensity of the PCR product observed on the agarose gel, representing amplification of the deletion template (Fig. 5B). Therefore, to increase the proportion of edited cells in a population, ATTO-labelled tracrRNA was included in the RNP transfection and the cells were sorted using fluorescence-activated cell sorting (FACS). The cells were detached from the plate 24 h after transfection, counterstained with DAPI (to indicate cell viability) and separated by their ATTO positive (41,096 cells) or ATTO negative (336,400 cells) status by an Aria FACS machine. These results indicate that 12.2% of the cells were successfully transfected with enough ATTO-labelled tracrRNA to meet the FACS sorting threshold level.
The ATTO positive population of cells that had been edited and sorted, the RNP only edited and unsorted cells, and unedited control cells were grown for approximately three cell doublings before being plated onto Matrigel-coated wells and cultured for 2 wk. To determine if FACS increased the proportion of edited cells, PCR using primers flanking the expected CRISPR-Cas9-mediated deletion in DGAT1 was performed on each cell population.
The full-length PCR product was separated by gel electrophoresis from the shorter fragments, which indicate dual guide mediated deletion. Figure 8B shows a more prominent PCR band corresponding to the deletion in the ATTO positive, edited and sorted cell population (Fig. 8B, lane 4), compared with the knock-out band seen in the original unsorted, edited cell population (Fig. 8B, lane 2).
To quantify the proportion of edited alleles in each population, the PCR amplicons were sequenced via minION (Fig. 8C). The editing frequency was calculated by averaging the read depth across the deletion site compared to the unedited genomic context. minION sequencing of DGAT1 PCR amplicons can be found here: PRJNA862600. Sequencing of the amplicons derived from the ATTO-labelled tracrRNA unsorted cells (Fig. 8B, lane 2) demonstrated a 17.42% editing efficiency. The sequencing from the same cells that were ATTO positive after sorting using FACS (Fig. 8B, lane 4) showed 60% editing. As expected, the negatively sorted cells from the same pool (ATTO negative), had a much lower editing frequency of 3.75% (Fig. 8B, lane 4) compared to the pre-sorted pool of cells. The editing frequency of the unsorted cells containing only the RNP complex (No ATTO label) was 8.58%. Sorting the cells containing ATTO-Labelled tracrRNA improved the proportion of edited cells in the population by 42.58%. A preliminary experiment assessed the maintenance of the expression of lactogenic genes GPAT4 and MGST1 after editing and sorting had occurred, and cells were grown on plastic. Figure 9 demonstrates that after the primary cells had undergone editing and sorting, they maintained expression of both GPAT4 and MGST1 genes to the same level or higher than the cells which had not undergone these methods. These results suggest that the primary cells can maintain their lactogenic gene expression profile even after the dual disturbance of lipofection and sorting.
To determine if the deletion of the targeted region of DGAT1 exon 1 influenced the abundance of functional DGAT1 mRNA, qPCR was conducted on RNA extracted from the sorted and unsorted cells at passage 5 grown on Matrigel and on plastic (Fig. 10). When grown on plastic, DGAT1 expression was reduced in cells that had undergone DGAT1 knock-out editing and ATTO positive sorting (ATTO + Sorted on plastic; relative expression: 2.36 ± 0.1) compared to the unsorted DGAT1 knock-out edited cells (RNP only on plastic; relative expression: 4.63 ± 0.12) and wild type cells (Wild-type unsorted on plastic; relative expression: 3.45 ± 0.08). Similarly, DGAT1 expression was reduced in edited and sorted ATTO-positive cells grown on Matrigel (ATTO + Sorted on Matrigel; relative expression: 5.8 ± 0.11) compared with edited but unsorted cells grown on Matrigel (RNP only on Matrigel; relative expression: 14.04 ± 0.06). Potential off-target regions in the ATTO plus RNP transfected cells (well A1) were analysed by PCR amplification and Sanger sequencing. Supplementary Figure S3 shows the Sanger traces of the potential guide binding regions. There was no off-target editing found at any of the top four ranked potential off-target sequences. We see the sequence traces of the edited cells are an exact match to the wild-type sequence, across the off-target regions, indicating no cutting at these locations.
Discussion
The culturing of primary cells in vitro is a technique that facilitates the observation of transcriptomic and phenotypic changes in cells in response to many different experimental perturbations — including gene editing (Ge et al. 2021). Cell models with physiological features closely representing the tissue in question are more desirable for this purpose. Investigating the influence of genetic variants or the introduction of transgenes on milk production traits is an essential tool allowing, for example, the identification of markers for productivity selection (Lopdell et al. 2017; Pegolo et al. 2018). Targeted genetic changes introduced by CRISPR-Cas9 editing in primary cell models may help to elucidate the effects of these variants on phenotypes of interest including fat and protein percentage and overall milk yield. The major limitation of cell culture models of lactating mammary tissue is the loss of expression of lactation-related genes (especially those that encode milk proteins) after only a few passages (Jedrzejczak and Szatkowska 2014). This characteristic puts a time constraint on experiments and limits investigation to early cell passages. It may also decrease the reproducibility of variant effects between primary lines that do not have stable gene expression.
Characterisation by ICC of the four primary cell lines derived from tissue biopsies revealed they were all predominantly epithelial in nature (Fig. 3). Additionally, there was no evidence of fibroblasts outcompeting epithelial cells during our experiments, which may happen as the extraction process does not remove all fibroblasts. All four primary pbMEC cell lines were successfully transfected using CRISPRMAX lipofectamine reagent, and delivery of guide RNAs in a ribonuclear complex resulted in targeted gene editing. The gene expression profile of the key milk-related genes grown on plastic over three passages was found to be similar to previously characterised pbMEC lines, where there is a rapid loss of expression of milk proteins. In particular, casein and beta-lactoglobulin expression decreased substantially after only a few passages (Fig. 4). As others have reported, we found that when cultured on Matrigel with prolactin, milk protein gene expression is maintained or re-established, at least to some extent, for four passages and multiple weeks of culturing (Sun et al. 2005; Riley et al. 2010). In addition, cells grown on Matrigel formed 3D structures visible under the light microscope, as previously reported (Rose et al. 2002; Anand 2012).
We have demonstrated the use of FACS to enrich for a population of gene-edited primary cells based on their ability to uptake CRISPR reagents. This has wide-ranging applications when considering gene editing. The methods described here provide a system of protocols for maintaining the expression of milk protein and fat synthesis genes for multiple weeks, in addition to enhancing the proportion of edited cells in a population. When combined, these methods may give greater certainty that changes in gene expression after gene editing will be observable and more physiologically relevant compared to primary cells grown on plastic or immortalised cell lines. This technique may be applied to many different cell types to investigate any type of genetic perturbation.
We chose DGAT1 as a functional knock-out gene target for the development of the methodology as it is a critical enzyme for triglyceride synthesis, and variants within this gene are known to significantly influence fat yield in milk (Fink et al. 2020). Specifically, DGAT1 is responsible for the addition of the third fatty acid chain converting diacylglycerol to triacylglycerol. DGAT1 expression was relatively high and consistent in cells grown on Matrigel, allowing resolution of the changes in gene expression after deletion editing. Post-editing FACS improved the proportion of pbMEC cells with the knock-out from 17.4 to 60%. The sorted edited cells had 41% lower DGAT1 relative expression than the unsorted cells. Edited cells grown on Matrigel showed a significantly higher level of DGAT1 expression compared to their equivalent population growing on plastic. We also demonstrated that the cells could go through at least four cell doublings and still recover their DGAT1 gene expression. Growth on Matrigel also allowed initial gene expression levels to be maintained for longer, which dramatically expanded the time frame during which we could assess the effects of gene editing on edited primary cell cultures.
Other attempts to create a CRISPR-Cas9 knock-out in lactating mammary epithelial lines have yielded lower editing efficiencies than the presented results. Zhang et al. 2021, overcame this by isolating a single Goat mammary epithelial cell (GMEC) TPH1 knock-out clone that was confirmed to have a knock-out in both alleles before further experiments were conducted to assess intracellular calcium levels (Zhang et al. 2021). These cells would have gone through tens of doublings before reaching sufficient numbers to be used in experiments which may limit the investigation of milk protein gene expression. Other authors have used a similar approach to assess the effect of microRNA and gene knock-outs on the expression of fatty acid metabolism-related genes (Tian et al. 2018; Huang et al. 2020). They observed changes in the expression of fatty acid synthesis genes, including DGAT1, in their primary GMEC clones with the knock-out compared to a WT clone. It is unclear how physiologically relevant these results are, given the high number of cell doubling these cell populations have gone through and the culturing of the cells on plastic.
The efficiency of the introduction of specific mutations by homology-directed repair (HDR) is likely to be lower than the efficiency of introducing knock-out deletions (Prill and Dawson 2020). Editing rates are affected by the delivery method, the target gene sequence, and the HDR template used (Prill and Dawson 2020). However, methods such as the FACS sorting applied here should have relevance to HDR-based methods and result in editing-enriched populations.
In summary, the methods and results presented here demonstrate the potential for creating a CRISPR-Cas9-edited primary cell line with a high proportion of edited cells that can maintain initial levels of lactogenic gene expression. The combination of FACS and culturing of cells on Matrigel enables the investigation of the impacts of variants introduced via gene editing on the expression of central lactogenic genes in primary cells for extended periods.
Data availability
Raw qPCR data and calculations can be found at https://doi.org/10.7910/DVN/YATECZ. minION sequencing of DGAT1 PCR amplicons can be found here: PRJNA862600.
Abbreviations
- pbMEC :
-
Primary bovine mammary epithelial cell
- CRISPR :
-
Clustered regularly interspaced short palindromic repeats
- crRNA :
-
CRISPR ribonucleic acid
- DGAT1 :
-
Diacylglycerol O-acyltransferase 1
- gRNA :
-
Guide ribonucleic acid
- MAC-T :
-
Primary bovine mammary alveolar cells immortalised by stable transfection with SV-40 large T-antigen
- SpCas9 :
-
(CRISPR)-associated S. pyogenes nuclease Cas9
- tracrRNA :
-
Trans-activating CRISPR ribonucleic acid
- FACS :
-
Fluorescence-assisted cell sorting
- NHEJ :
-
Non-homologous end joining
- DMEM :
-
Dulbecco’s Modified Eagle Medium
- DM :
-
Differentiation media
- ICC :
-
Immunocytochemistry
- RNP :
-
Ribonuclear protein
- HDR :
-
Homology-directed repair
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Open Access funding enabled and organized by CAUL and its Member Institutions Ministry of Business, Innovation and Entrepreneurship: C10 519: Primary cell isolation.
Ministry of Business, Innovation and Entrepreneurship: 5,000,599.
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J.M, E.M, and R.S conceived of the experimental design. J.M and E.M carried out the experiments. T.C completed the FACS sorting of the cells. J.M, E.M, A.T, R.S, M.L, and T.L contributed to analysis of the data and interpretation of results. J.M wrote the manuscript with support from E.M, A.T, M.L, T.L, and R.S. pbMECs were initially obtained by A.M and M.B.
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The animals were obtained from the Ruakura Dairy Farm and were slaughtered in accordance with MPI regulations at the AgResearch abattoir on the Ruakura Farm, under the AgResearch ethics approval number 3925. The Ruakura Animal Ethics Committee approved the study. None of the authors were involved in the slaughtering of the animals. These methods are in accordance with the ARRIVE guidelines.
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Moody, J., Mears, E., Trevarton, A.J. et al. Successful editing and maintenance of lactogenic gene expression in primary bovine mammary epithelial cells. In Vitro Cell.Dev.Biol.-Animal 59, 316–330 (2023). https://doi.org/10.1007/s11626-023-00762-6
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DOI: https://doi.org/10.1007/s11626-023-00762-6