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Maintenance of the EHS sarcoma and Matrigel preparation

  • Preparation Of Cell Adhesive Receptors
  • Published:
Journal of tissue culture methods

Summary

Matrigel is a reconstituted basement membrane extract which is rich in laminin, growth factors, entactin/nidogen, type IV collagen, and heparan sulfate proteoglycan (perlecan). This basement membrane extract can be used for promotion and maintenance of a differentiated phenotype in cell cultures, as well as for a variety of in vitro and in vivo uses. The Engelbreth-Holm-Swarm (EHS) sarcoma is a rich source of both individual basement membrane components and Matrigel. Maintenance of the EHS tumor in mice and its subsequent harvesting for the preparation of Matrigel are described.

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Authors and Affiliations

  1. Laboratory of Developmental Biology, National Institute of Dental Research, NIH, Building 30/430, 20892, Bethesda, MD, USA

    Maura C. Kibbey

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  1. Maura C. Kibbey
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Kibbey, M.C. Maintenance of the EHS sarcoma and Matrigel preparation. Journal of Tissue Culture Methods 16, 227–230 (1994). https://doi.org/10.1007/BF01540656

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  • DOI: https://doi.org/10.1007/BF01540656

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