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Establishment and characterization of a bovine mammary epithelial cell line with unique properties

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Summary

Clonal cell lines (BME-UV) were established from primary epithelial cells by stable transfection with a plasmid, carrying the sequence of the simian virus 40 early region mutant tsA58, encoding the thermolabile large T antigen. The BME-UV cells have undergone more than 300 population doublings and produce intranuclear large T antigen. At low confluency, growing islands of cells are apparent exhibiting the characteristic cobblestone morphology of epithelial cells. The BME-UV cells expressed functional markers such as microvilli and desmosomes and biochemical markers of mammary epithelial cells such as a repertoire of cytokeratins. The BME-UV cells are capable of synthesizing low levels of α-lactalbumin and α8l (50 ng/ml of medium/24 h). One of the cell lines, BME-UV1 showed enhanced proliferation in the presence of epidermal growth factor (EGF) and insulinlike growth factor I (IGF-I). The BME-UV1 cell line is the only known bovine mammary epithelial cell line responsive to EGF. The BME-UV cells grown on collagen at low confluency are capable of developing very long projections that most likely allow for communication between cells at a distance from each other. The BME-UV cells may become a valid model system to examine bovine mammary epithelial proliferation and differentiation and cell-to-cell communication.

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Authors and Affiliations

  1. Department of Animal and Food Sciences, University of Vermont, 05405, Burlington, Vermont

    Boris Zavizion, Marilyn van Duffelen & Ioannis Politis

  2. Department of Microbiology and Molecular Genetics, University of Vermont, 05405, Burlington, Vermont

    Warren Schaeffer

Authors
  1. Boris Zavizion
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  2. Marilyn van Duffelen
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  3. Warren Schaeffer
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  4. Ioannis Politis
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Zavizion, B., van Duffelen, M., Schaeffer, W. et al. Establishment and characterization of a bovine mammary epithelial cell line with unique properties. In Vitro Cell.Dev.Biol.-Animal 32, 138–148 (1996). https://doi.org/10.1007/BF02723679

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  • DOI: https://doi.org/10.1007/BF02723679

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