Abstract
Potato (Solanum tuberosum L.) plantlets were conditioned in tissue culture to produce more minitubers when planted in a greenhouse. Tissue-cultured potato plantlets, varieties Coliban and Russet Burbank, were grown for 4, 10, or 12 weeks under three temperature regimes (22/18 °C, 16/10 °C, and 10/2 °C day/night) and a photoperiod of 16, 12, or 8 h in different stages of growth. Duration, temperature, and photoperiod of the in vitro period affected plantlet morphology and the total number of minitubers produced per plant in a greenhouse. Extending the growing period and introducing a hardening period with low temperatures (16/10 °C) during the in vitro production stage resulted in 97% more minitubers (4.94 vs 2.50 minitubers per plant for the control) in variety Coliban and up to 71% (6.50 vs 3.80 minitubers per plant for the control) in variety Russet Burbank. The total number of minitubers produced per plant did not change significantly for both varieties when a shorter photoperiod was used instead of the standard 16-h day during the growing period in vitro. Results presented in this experiment demonstrate that the number of minitubers can be substantially increased through the introduction of an induction period as an interstage between the in vitro stages of potato plantlet production and minituber production.
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References
Akita M, Takayama S (1994) Stimulation of potato (Solanum tuberosum L.) tuberisation by semi continuous liquid medium surface level control. Plant Cell Rep 13:184–187
Allen JR, Knutson KW (1982) The effect of cold storage on the growth and development of tissue culture propagated potato plantlets. 66th Annual PAA meeting, Monterey, pp. 459 (Abstract)
Asiedu SK, Astatkie T, Yiridoe EK (2003) The effect of seed–tuber physiological age and cultivar on early potato production. J Agron Crop Sci 189:176–184
Bennett SM, Tibbitts TW, Cao W (1991) Diurnal temperature fluctuation effects on potatoes grown with 12 h photoperiod. Am Potato J 68:81–86
Bodlaender KBA (1963) Influence of temperature, radiation and photoperiod on development and yield. In: Ivins JD, Milthorpe FL (eds) The growth of the potato. Butterworth, London, pp 199–210
Burt RL (1964) Influence of short periods of low temperature on tuber initiation in the potato. Eur Potato J 7:197–208
Chapman HW (1958) Tuberisation in potato plant. Plant Physiol 11:215–224
Dodds JH, Silva-Rodriguez D, Tovar P (1992) Micropropagation of potato. In: Bajaj PS (ed) Biotechnology in agriculture and forestry, vol. 19. High-tech and micropropagation III. Springer, Berlin, pp 92–106
Ewing EE (1981) Heat stress and the tuberisation stimulus. Am Potato J 58:31–49
Ewing EE (1985) Cuttings as simplified models of the potato plant. In: Li PH (ed) Potato physiology. Academic, Orlando, pp 154–207
Ewing EE, Struik PC (1992) Tuber formation in potato: induction, initiation and growth. Hortic Rev 14:89–197
Ewing EE, Wareing PF (1978) Shoot, stolon and tuber formation on potato (Solanum tuberosum L.) cuttings in response to photoperiod. Plant Physiol 61:348–353
Gregory LE (1956) Some factors for tuberisation in the potato. Ann Bot 41:281–288
Hare PD, Cress WA, van Staden J (1997) The involvement of cytokinins in plant responses to environmental stress. Plant Growth Regul 23:79–103
Hussey G, Stacey NJ (1981) In vitro propagation of potato (Solanum tuberosum L.). Ann Bot 48:787–796
Hussey G, Stacey NJ (1984) Factors affecting the formation of in vitro tubers of potato (Solanum tuberosum L.). Ann Bot 53:565–578
Jackson DJ (1999) Multiple signaling pathways control tuber induction in potato. Plant Physiol 119:1–8
Knutson KW (1988) Implication of new technologies for seed potato certification programs and seed growers. Am Potato J 65:229–235
Lommen WJM (1999) Causes for low tuber yields of transplants from in vitro potato plantlets of early cultivars after field planting. J Agric Sci 133:275–284
Lommen WJM, Struik PC (1994) Field performance of potato minitubers with different fresh weights and conventional seed tubers: crop establishment and yield formation. Potato Res 37:301–313
Lorenzen JH, Ewing EE (1990) Changes in tuberisation and assimilate partitioning in potato (Solanum tuberosum) during the first 18 days of photoperiod treatment. Ann Bot 66:457–464
Madec P, Perennec P (1962) Les relations entre l’induction de la tubérisation et la croissance chez la plante de pomme de terre (Solanum tuberosum L.). Ann Physiol Vég 4:5–84
Marinus J (1985) In vitro multiplication of potatoes: description of methods and experience in the Netherlands. Centre for Agricultural Research (CABO), Wageningen, pp 1–21
Marinus J, Bodlaender KBA (1975) Response of some potato varieties to temperature. Potato Res 18:189–204
Menzel CM (1985) Tuberisation in potato at high temperatures: interaction between temperature and irradiance. Ann Bot 55:35–39
Mikitzel LJ, Knowles NR (1990) Effect of potato seed–tuber age on plant establishment and amelioration of age-linked effects with auxin. Plant Physiol 93:967–975
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
O’Brien J, Allen EJ, Bean JN, Griffith RL, Jones SA, Jones JL (1983) Accumulated day-degrees as a measure of physiological age and the relationships with growth and yield in early potato varieties. J Agric Sci 101:613–631
Perennec P (1966) Induction de la tubérisation et inhibition des bourgeons chez la pomme de terre (Solanum tuberosum L.). Bull Soc Franç Physiol Vég 12:175–192 (cited by Menzel 1983)
Pruski KW (2001) Micropropagation technology in early phases of commercial seed potato production. PhD Thesis, Wageningen University, Wageningen, The Netherlands. pp 166 (Abstract)
Pruski KW, Astatkie T, Duplessis P, Nowak J, Struik PC (2003) Manipulation of microtubers for direct field utilization in seed production. Am J Potato Res 80:173–181
Seabrook JEA, Coleman S, Levy D (1993) Effect of photoperiod on in vitro tuberisation of potato (Solanum tuberosum L.). Plant Cell Tiss Organ Cult 34:43–51
Seabrook JEA, Percy JE, Douglass LK, Tai GCC (1995) Photoperiod in vitro affects subsequent yield of greenhouse-grown potato tubers. Am Potato J 72:365–373
Snyder RG, Ewing EE (1989) Interactive effects of temperature, photoperiod and cultivar on tuberisation of potato cuttings. Hortic Sci 24:336–338
Steward FC, Moreno U, Roca WM (1981) Growth, form and composition of potato plants as affected by environment. Ann Bot 48:1–45
Struik PC, Wiersema SG (1999) Seed potato technology. Wageningen Pers, Wageningen
Struik PC, Vreugdenhil D, van Eck HJ, Bachem CW, Visser RGF (1999) Physiological and genetic control of tuber formation. Potato Res 42:313–331
Sudha G, Ravishankar GA (2002) Involvement and interaction of various signaling compounds on the plant metabolic events during defense response, resistance to stress factors, formation of secondary metabolites and their molecular aspects. Plant Cell Tiss Organ Cult 71:181–212
Tadesse M, Lommen WJM, Struik PC (2000) Effects of in vitro treatments on leaf area growth of potato transplants during acclimatization. Plant Cell Tiss Organ Cult 61:59–67
Tibbitts TW, Bennett SM, Cao W (1990) Control of continuous irradiation injury on potatoes with daily cycling. Plant Physiol 93:409–411
Tovar P, Estrada R, Schilde-Rentschler L, Dodds JH (1985) Induction and use of in-vitro potato tubers. CIP Circular 13(4):1–5
van Dam J, Kooman PL, Struik PC (1996) Effects of temperature and photoperiod on early growth and final number of potato tubers in potato (Solanum tuberosum L.). Potato Res 39:51–62
Wan P, Hu C (1982) In vitro mass tuberisation and virus-free seed-potato production in Taiwan. Am Potato J 59:33–37
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Milinkovic, M., Horstra, C.B., Rodoni, B.C. et al. Effects of Age and Pretreatment of Tissue-Cultured Potato Plants on Subsequent Minituber Production. Potato Res. 55, 15–25 (2012). https://doi.org/10.1007/s11540-011-9203-4
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DOI: https://doi.org/10.1007/s11540-011-9203-4