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The fungal endophyte Epichloë gansuensis increases NaCl-tolerance in Achnatherum inebrians through enhancing the activity of plasma membrane H+-ATPase and glucose-6-phosphate dehydrogenase

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An Erratum to this article was published on 17 April 2021

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Abstract

Salt stress negatively affects plant growth, and the fungal endophyte Epichloëgansuensis increases the tolerance of its host grass species, Achnatherum inebrians, to abiotic stresses. In this work, we first evaluated the effects of E. gansuensis on glucose-6-phosphate dehydrogenase (G6PDH) and plasma membrane (PM) H+-ATPase activity of Achnatherum inebrians plants under varying NaCl concentrations. Our results showed that the presence of E. gansuensis increased G6PDH, PM H+-ATPase, superoxide dismutase and catalase activity to decrease O2•−, H2O2 and Na+ contents in A. inebrians under NaCl stress, resulting in enhanced salt tolerance. In addition, the PM NADPH oxidase activity and NADPH/NADP+ ratios were all lower in A. inebrians with E. ganusensis plants than A. inebrians plants without this endophyte under NaCl stress. In conclusion, E. gansuensis has a positive role in improving host grass yield under NaCl stress by enhancing the activity of G6PDH and PM H+-ATPase to decrease ROS content. This provides a new way for the selection of stress-resistant and high-quality forage varieties by the use of systemic fungal endophytes.

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Acknowledgements

This work was financially supported by the National Basic Research Program of China (2014CB138702), the Joint Fund of the National Natural Science Foundation of China and the Karst Science Research Center of Guizhou Province (U1812401), Changjiang Scholars and Innovative Research Team in University (IRT_17R50), Lanzhou University “Double First-Class” guiding special project-team construction fund-scientific research start-up fee standard (561119206), the National Natural Science Foundation of China (31901378), Guizhou Education Department Program (Qianjiaohe-KY-2018-130), Major Science and Technology Sub-project of Guizhou Science and Technology Program (Qiankehe-2019-3001-2).

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  1. State Key Laboratory of Grassland Agro-ecosystems; Center for Grassland Microbiome; Key Laboratory of Grassland Livestock Industry Innovation; Ministry of Agriculture and Rural Affairs; Engineering Research Center of Grassland Industry; Ministry of Education; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730000, China

    Jianfeng Wang, Wenpeng Hou, Chao Xia, Tao Chen & Zhibiao Nan

  2. Retired scientist of AgResearch, Grasslands Research Centre, Private Bag 11-008, Palmerston North, 4442, New Zealand

    Michael J. Christensen

  3. College of Grassland Agriculture, Northwest A&F University, Yangling, 712100, China

    Zhixin Zhang

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  1. Jianfeng Wang
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The fungal endophyte Epichloë gansuensis increases NaCl-tolerance in Achnatherum inebrians through enhancing the activity of plasma membrane H+-ATPase and glucose-6-phosphate dehydrogenase

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Wang, J., Hou, W., Christensen, M.J. et al. The fungal endophyte Epichloë gansuensis increases NaCl-tolerance in Achnatherum inebrians through enhancing the activity of plasma membrane H+-ATPase and glucose-6-phosphate dehydrogenase. Sci. China Life Sci. 64, 452–465 (2021). https://doi.org/10.1007/s11427-020-1674-y

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  • DOI: https://doi.org/10.1007/s11427-020-1674-y

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