Abstract
Mangrove crabs are ecosystem engineers through their bioturbation activity. On Mayotte Island, the abundance of Neosarmatium africanum decreased in wastewater-impacted areas. Previous analyses showed that global crab metabolism is impacted by wastewater, with a burst in O2 consumption that may be caused by osmo-respiratory trade-offs since gill functioning was impacted. As the hepatopancreas is a key metabolic organ, the purpose of this study was to investigate the physiological effects of wastewater and ammonia-N 5-h exposure on crabs to better understand the potential trade-offs underlying the global metabolic state. Catalase, superoxide dismutase, glutathione S-transferase, total digestive protease, and serine protease (trypsin and chymotrypsin) activities were assessed. Histological analyses were performed to determine structural modifications. No effect of short-term wastewater and ammonia-N exposure was found in antioxidant defenses or digestive enzyme activity. However, histological changes of B-cells indicate an increase in intracellular digestive activity through higher vacuolization processes and tubule dilation in wastewater-exposed crabs.
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The authors are thankful to the Syndicat Intercommunal d'Eau et d'Assainissement de Mayotte (SIEAM), and especially to Kissimati Abdallah for her help in the field.
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ES, JHL, and GR contributed to the study conceptualization and design, and supervised the research. Experimental design, material preparation, and data collection were performed by DT. DT and LM performed the assessment of antioxidant enzyme activities. DM performed the assessment of digestive enzymes activities and the pretreatment of the outcome data. ML realized the cut sections and staining of the hepatopancreas tubules. LM analyzed the histological sections and the outcomes of the antioxidant enzymes activities and realized the statistical analyses of the manuscript dataset. The first draft of the manuscript was written by LM, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Mégevand, L., Martínez-Alarcón, D., Theuerkauff, D. et al. The hepatopancreas of the mangrove crab Neosarmatium africanum: a possible key to understanding the effects of wastewater exposure (Mayotte Island, Indian Ocean). Environ Sci Pollut Res 28, 60649–60662 (2021). https://doi.org/10.1007/s11356-021-14892-5
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DOI: https://doi.org/10.1007/s11356-021-14892-5