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Subcellular distribution of zinc and cadmium in the hepatopancreas and gills of the decapod crustacean Penaeus indicus

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Abstract

The decapod crustacean Penaeus indicus accumulated Cd and Zn in different subcellular compartments of hepatopancreas and gill cells. Most of the Cd and part of the Zn accumulates within the soluble fraction of the cells, while the remainder of the Zn is found in insoluble inclusions, associated with P, Ca, Mg and Si in B-, F- and R-cells in the hepatopancreas, and haemocytes, nephrocytes and epithelial cells in the gills. No presence of Cd was observed in metal-rich inclusions in any cell analysed. Metallothionein-like proteins (MTLP), analysed by differential pulse polarography, were present in the hepatopancreas (12–18 mg g−1) and gills (7–8 mg g−1) of metal-exposed prawns. Binding to MTLP is the detoxification mechanism for cadmium, while the detoxification of zinc involves both binding to MTLP and incorporation into insoluble metal-rich inclusions.

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Acknowledgments

This work was supported by funding to GN-N from CONACyT and SEP-Mexico, and by funding to Dr. C. Amiard-Triquet (Service d’Ecotoxicologie, ISOMer, Nantes, France). It is a pleasure to acknowledge the considerable technical support of Keith Pell (Queen Mary College, University of London), and Dr. Alex Ball (The Natural History Museum, London), and for training and advice on the X-ray microanalysis and TEM analysis, respectively. We are also grateful for the technical support provided by CEREA-UCO (Angers, France).

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Authors and Affiliations

  1. Department of Zoology, The Natural History Museum, Cromwell Road, London, SW7 5BD, UK

    G. Nunez-Nogueira & P. S. Rainbow

  2. Centre d’étude et de Recherche sur les écosystèms aquatiques, Université Catholique de l’ouest, 44 Rue Rabelais, BP 808, 49008, Angers Cedex 01, France

    C. Mouneyrac

  3. Service d’Ecotoxicologie, CNRS-GDR 1117, ISOMer, SMAB, 2 Rue de la Houssinière, BP 92208, 44322, Nantes Cedex 3, France

    J. C. Amiard

  4. Laboratorio de Contaminación Marina, Instituto de Ciencias del Mar y Limnología, UNAM, Circuito Exterior S/N. 04510, México DF, Mexico

    G. Nunez-Nogueira

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  1. G. Nunez-Nogueira
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  2. C. Mouneyrac
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Correspondence to P. S. Rainbow.

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Communicated by J.P. Thorpe, Port Erin

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Nunez-Nogueira, G., Mouneyrac, C., Amiard, J.C. et al. Subcellular distribution of zinc and cadmium in the hepatopancreas and gills of the decapod crustacean Penaeus indicus . Mar Biol 150, 197–211 (2006). https://doi.org/10.1007/s00227-006-0350-0

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